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Characterization of acid- and pepsin-soluble collagens from flatfish skin

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Abstract

Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from flatfish skin were characterized. The yield of PSC was 85.5%, which was higher than that of ASC at 57.3%. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns showed that both the ASC and PSC were consisted of α1 and α2 chains, and β-chain. According to the results of Fourier transform infrared (FTIR) spectroscopy and hydroxylation, no difference in the helical structure between ASC and PSC was found. Thermal denaturation temperature (TDT) of flatfish skin collagen was 26.6°C for ASC and 26.7°C for PSC, which were similar to those of temperate fishes, while were lower compared to tropical fishes, such as the conger eel and Nile perch. There was no solubility difference between ASC and PSC at various pH and NaCl concentrations. Therefore, PSC will be preferable compared to ASC for commercial uses because of its higher yield.

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Authors and Affiliations

  1. Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University, Tongyeong, Gyeongnam, 650-160, Korea

    Jae Hyoung Lee, Hyung Jun Kim, Seong Joon Jee & Jin-Soo Kim

  2. Department of Food Science and Nutrition /Institute of Marine Industry, Gyeongsang National University, Jinju, Gyeongnam, 660-701, Korea

    Min Soo Heu

  3. Aqua Green Technology Co., Ltd., Jeju, Jeju, 690-150, Korea

    Jung Suck Lee

  4. Department of Marine Biotechnology, Cheju National University, Jeju, Jeju, 690-75 6, Korea

    You-Jin Jeon

  5. Department of Biochemistry, Memorial University of Newfoundland, St. John’s, NL, A1B 3X9, Canada

    Fereidoon Shahidi

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  1. Min Soo Heu
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  3. Hyung Jun Kim
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  8. Jin-Soo Kim
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Correspondence to Jin-Soo Kim.

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Heu, M.S., Lee, J.H., Kim, H.J. et al. Characterization of acid- and pepsin-soluble collagens from flatfish skin. Food Sci Biotechnol 19, 27–33 (2010). https://doi.org/10.1007/s10068-010-0004-3

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  • DOI: https://doi.org/10.1007/s10068-010-0004-3

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