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Association of a miRNA-binding site polymorphism in IL-16 gene with disease risk and clinical characteristics of rheumatoid arthritis and systemic lupus erythematosus

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Abstract

Introduction

/objectives.

Single nucleotide polymorphisms (SNPs) located at the 3′-UTR region of the target genes of microRNAs (miRNAs) can dysregulate their expression via disrupting the binding site of miRNAs. Interleukin-16 (IL-16) is involved in the pathogenesis of rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). In the current study, we assessed the possible association between rs1131445 polymorphism in IL-16 gene with risk and clinical characteristics of RA and SLE in the Iranian population.

Methods

In this case–control study, 120 patients with RA, 120 patients with SLE, and 120 unrelated healthy subjects were collected to estimate rs1131445 (T > C) polymorphism in IL-16 gene using real-time PCR high-resolution melting (HRM) method.

Results

Our results demonstrated considerable associations between TC genotype and C allele of rs1131445 with enhanced risk of RA (ORfor TC genotype = 3.01; 95%CI [1.667–5.526], P < 0.001; ORfor C allele = 1.96; 95%CI [1.314–2.941], P < 0.001). Besides, there was a marginal association between CC genotype and increased risk of RA (P: 0.031). However, there was an insignificant correlation between genotypes and allele frequencies of rs1131445 with incidence risk of SLE (P > 0.05). Moreover, stratification analysis indicated that the C allele in rs1131445 was linked with disease activity–associated laboratory parameters such as CRP and ESR in both RA and SLE patients, as well as the higher incidence of neurological symptoms in SLE subjects (P < 0.05).

Conclusion

These results proposed a significant association between IL-16 polymorphism and augmented risk of RA and clinical characteristics of RA and SLE.

Key Points

• Single nucleotide polymorphism (rs1131445) in miRNA -binding sites which is located in 3′ˊUTR of the IL-16 gene could be associated with RA and SLE risk by dysregulation of IL-16 expression.

• Our findings proposed a significant association between IL-16 polymorphism and augmented risk of RA and clinical characteristics of RA and SLE

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Acknowledgements

We would like to appreciate any support provided by Isfahan University of Medical Sciences and Aja University of Medical Science.

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Authors and Affiliations

  1. Fetal Health Research Center, Hope Generation Foundation, Tehran, Iran

    Somayeh Zeinalzadeh, Emran Esmaeilzadeh & Naeim Ehtesham

  2. Department of Medical Genetics and Molecular Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Nahid Kheradmand

  3. Faculty of Basic Sciences, Central Tehran Branch, Islamic Azad University, Isfahan, Iran

    Ghazal Rasouli

  4. Division of Rheumatology, Department of Internal Medicine, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran

    Bahram Pakzad

  5. Research Center for Cancer Screening and Epidemiology, AJA University of Medical Sciences, Tehran, Iran

    Javad Behroozi

  6. Department of Genetics and Advanced Medical Technology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran

    Javad Behroozi

  7. Toxicology Research Center, Aja University of Medical Sciences, Tehran, Iran

    Mohsen Chamanara

  8. Department of Pharmacology, School of Medicine, Aja University of Medical Sciences, Tehran, Iran

    Mohsen Chamanara

  9. Trauma Research Center, Aja University of Medical Sciences, Tehran, Iran

    Mojtaba Yousefi Zoshk

  10. School of Medicine, Aja University of Medical Science, Tehran, Iran

    Mehrdad Nasrollahzadeh Sabet

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  1. Somayeh Zeinalzadeh
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Correspondence to Mehrdad Nasrollahzadeh Sabet.

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Zeinalzadeh, S., Kheradmand, N., Rasouli, G. et al. Association of a miRNA-binding site polymorphism in IL-16 gene with disease risk and clinical characteristics of rheumatoid arthritis and systemic lupus erythematosus. Clin Rheumatol 41, 2189–2196 (2022). https://doi.org/10.1007/s10067-022-06131-0

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