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Expression of human endogenous retrovirus clone 4-1 may correlate with blood plasma concentration of anti-U1 RNP and anti-Sm nuclear antibodies

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Abstract

The transcription of human endogenous retrovirus E (HERV-E) clone 4-1 was determined in peripheral blood mononuclear cells (PBMC) of patients with systemic lupus erythematosus (SLE). However, the contribution of HERV-E clone 4-1 expression in the development of SLE remains unclear. Blood plasma and PBMC from 55 patients with SLE and a control group of 35 healthy individuals were collected. Blood plasma concentration of five antinuclear antibodies including anti-U1 ribonucleoprotein (RNP), anti-Sm, anti-Scl-70, anti-single-stranded DNA (ssDNA), and anti-double-stranded DNA (dsDNA) was analyzed by enzyme-linked immunosorbent assay (ELISA). Total RNA was isolated from PBMC and reverse transcribed into cDNA. The number of copies of HERV-E clone 4-1 gag transcript in PBMC was determined by real-time quantitative polymerase chain reaction (RQ-PCR) analysis. Spearman statistical analysis indicated that blood plasma concentrations of anti-U1 RNP and anti-Sm antibodies may correlate with PBMC transcript levels of HERV-E clone 4-1 gag sequence (R=0.775, p<0.000001; R=0.698, p<0.000001, respectively). Our observations suggest that the expression of HERV-E clone 4-1 might be associated with production of anti-U1 RNP and anti-Sm antibodies in patients with SLE.

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Acknowledgments

Supported by a Grant No. 2PO5B01927 from the State Committee for Scientific Research (KBN). We would like to acknowledge J Prokop, MD, PhD and A. Osmola, MD from the Department of Dermatology of Karol Marcinkowski University of Medical Sciences, Poznań, for providing SLE patients.

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Authors and Affiliations

  1. School of Medicine, Yale University, New Haven, CT, USA

    Piotr C. Piotrowski

  2. Polish Academy of Sciences, Medical Research Center, Warsaw, Poland

    Piotr C. Piotrowski

  3. Department of Biochemistry and Molecular Biology, University of Medical Sciences, 6 Święcickiego Street, 60-781, Poznań, Poland

    Sergiusz Duriagin & Paweł P. Jagodzinski

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  1. Piotr C. Piotrowski
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  2. Sergiusz Duriagin
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  3. Paweł P. Jagodzinski
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Correspondence to Paweł P. Jagodzinski.

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Piotrowski, P.C., Duriagin, S. & Jagodzinski, P.P. Expression of human endogenous retrovirus clone 4-1 may correlate with blood plasma concentration of anti-U1 RNP and anti-Sm nuclear antibodies. Clin Rheumatol 24, 620–624 (2005). https://doi.org/10.1007/s10067-005-1123-8

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  • DOI: https://doi.org/10.1007/s10067-005-1123-8

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