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Suppression of NS3 and MP is important for the stable inheritance of RNAi-mediated Rice Stripe Virus (RSV) resistance obtained by targeting the fully complementary RSV-CP gene

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Molecules and Cells

Abstract

Rice stripe virus (RSV) is a viral disease that seriously impacts rice production in East Asia, most notably in Korea, China, and Japan. Highly RSV-resistant transgenic japonica rice plants were generated using a dsRNAi construct designed to silence the entire sequence region of the RSV-CP gene. Transgenic rice plants were inoculated with a population of viruliferous insects, small brown planthoppers (SBPH), and their resistance was evaluated using ELISA and an infection rate assay. A correlation between the expression of the RSV-CP homologous small RNAs and the RSV resistance of the transgenic rice lines was discovered. These plants were also analyzed by comparing the expression pattern of invading viral genes, small RNA production and the stable transmission of the RSV resistance trait to the T3 generation. Furthermore, the agronomic trait was stably transmitted to the T4 generation of transgenic plants.

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Authors and Affiliations

  1. National Institute of Crop Science, Rural Development Administration, Suwon, 441-857, Korea

    Hyang-Mi Park, Man-Soo Choi, Bong-Choon Lee, Myeong-Ki Kim, Yeon-Gyu Kim, Dong-Bum Shin & Yul-Ho Kim

  2. Department of Functional Crop, National Institute of Crop Science, Rural Development Administration, Milyang, 627-803, Korea

    Do-Yeon Kwak & Jong-Hee Lee

  3. School of Applied Bioscience, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, 702-701, Korea

    Soon-Ki Park

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  1. Hyang-Mi Park
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  2. Man-Soo Choi
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Correspondence to Yul-Ho Kim.

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These authors contributed equally to this work.

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Park, HM., Choi, MS., Kwak, DY. et al. Suppression of NS3 and MP is important for the stable inheritance of RNAi-mediated Rice Stripe Virus (RSV) resistance obtained by targeting the fully complementary RSV-CP gene. Mol Cells 33, 43–51 (2012). https://doi.org/10.1007/s10059-012-2185-5

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  • DOI: https://doi.org/10.1007/s10059-012-2185-5

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