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Her4-positive population in the tectum opticum is proliferating neural precursors in the adult zebrafish brain

  • Research Article
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Molecules and Cells

Abstract

Previous studies have shown that Notch signaling not only regulates the number of early differentiating neurons, but also maintains proliferating neural precursors in the neural tube. Although it is well known that Notch signaling is closely related to the differentiation of adult neural stem cells, none of transgenic zebrafish provides a tool to figure out the relationship between Notch signaling and the differentiation of neural precursors. The goal of this study was to characterize Her4-positive cells by comparing the expression of a fluorescent Her4 reporter in Tg[her4-dRFP] animals with a GFAP reporter in Tg[gfap-GFP] adult zebrafish. BrdU incorporation indicated that dRFP-positive cells were proliferating and a double labeling assay revealed that a significant fraction of the Her4-dRFP positive population was also GFAP-GFP positive. Our observations suggest that a reporter line with Notch-dependent gene expression can provide a tool to examine proliferating neural precursors and/or neuronal/glial precursors in the development of the adult nervous system to examine the model in which Notch signaling maintains proliferating neural precursors in the neural tube.

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Authors and Affiliations

  1. Department of Biology and GRAST, Chungnam National University, Daejeon, 305-764, Korea

    Seung-Hyun Jung, Jae-Ho Ryu & Cheol-Hee Kim

  2. Department of Genetic Engineering, Kyungpook National University, Daegu, 702-701, Korea

    Hyung-Seok Kim

  3. Department of Biotechnology, Division of Applied Chemistry and Biotechnology, Hanbat National University, Daejeon, 305-719, Korea

    Jung-Woo Gwak & Sang-Yeob Yeo

  4. National Cancer Center, Research Institute, Goyang, 410-769, Korea

    Young-Ki Bae

Authors
  1. Seung-Hyun Jung
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  2. Hyung-Seok Kim
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  3. Jae-Ho Ryu
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  4. Jung-Woo Gwak
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  5. Young-Ki Bae
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  6. Cheol-Hee Kim
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  7. Sang-Yeob Yeo
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Corresponding authors

Correspondence to Cheol-Hee Kim or Sang-Yeob Yeo.

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These authors contributed equally to the work.

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Jung, SH., Kim, HS., Ryu, JH. et al. Her4-positive population in the tectum opticum is proliferating neural precursors in the adult zebrafish brain. Mol Cells 33, 627–632 (2012). https://doi.org/10.1007/s10059-012-0091-5

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  • DOI: https://doi.org/10.1007/s10059-012-0091-5

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