Abstract
Although chalk aquifers are not too often associated with conduit flow, they are highly productive groundwater systems and, like limestone aquifers, they can be vulnerable to contamination when exposed to land use activities. The Danish carbonate rocks are generally recognized to be highly fractured and covered by thick Quaternary sediments. Fissure flow is pronounced, occurring in the upper 50–100 m due to Pleistocene glaciations. According to recently published maps of the distribution of karst in Europe, Denmark has no karst. However, this study concludes that karstified chalk and limestone aquifers are an important source of freshwater in Denmark. Four national datasets on karst features, groundwater flow, groundwater chemistry, and fish ecological quality ratio (EQR) data now indicate more heterogeneous structures and preferential flow pathways in the chalk and limestone aquifers than had been conceptualized and modelled with a national water resources groundwater/surface-water model in the recent past. This study provides new qualitative evidence that rapid and preferential flow of water and agrochemicals from the surface through thinner parts of the Quaternary cover layers, sinkholes and solution-enlarged fractures may likely impact the vulnerability of chalk and limestone aquifers. Additionally, due to the preferential flow system, some gaining streams discharged by karstified chalk and limestone aquifers show increased fish EQR values when using observed river daily discharge data instead of simulated daily discharge.
Résumé
Bien que les aquifères crayeux ne soient pas très souvent associés à un écoulement par conduit, ce sont des systèmes d’eau souterraine très productifs et, comme les aquifères calcaires, ils peuvent être vulnérables à la contamination lorsqu’ils sont exposés à des activités liées à l’occupation des terres. Les roches carbonatées danoises sont généralement reconnues comme étant fortement fracturées et recouvertes d’épais sédiments quaternaires. L’écoulement dans les fissures est prononcé, se produisant dans les 50–100 m supérieurs en raison des glaciations pléistocènes. D’après les cartes récemment publiées sur la distribution du karst en Europe, le Danemark n’a pas de karst. Cependant, cette étude conclut que les aquifères calcaires et crayeux karstifiés sont une source importante d’eau douce au Danemark. Quatre ensembles de données nationales sur les caractéristiques karstiques, l’écoulement des eaux souterraines, la chimie des eaux souterraines et les données sur le rapport de qualité écologique (RQE) des poissons indiquent à ce jour des structures plus hétérogènes et des voies d’écoulement préférentielles dans les aquifères de la craie et du calcaire que ce qui avait été conceptualisé et modélisé avec un modèle national des ressources en eau pour les eaux souterraines/de surface dans un passé récent. Cette étude fournit de nouvelles preuves qualitatives que l’écoulement rapide et préférentiel de l’eau et des produits agrochimiques depuis la surface à travers les parties les plus minces des couches de couverture du Quaternaire, les dolines et les fractures élargies par dissolution peuvent probablement avoir un impact sur la vulnérabilité des aquifères de craie et de calcaire. De plus, en raison du système d’écoulement préférentiel, certains cours d’eau alimentés par des aquifères calcaires et crayeux karstifiés présentent des valeurs EQR de poissons plus élevées lorsqu’on utilise les données de débit journalier de la rivière observée au lieu du débit journalier simulé.
Resumen
Aunque los acuíferos de creta no se asocian con demasiada frecuencia al flujo de conductos, son sistemas de aguas subterráneas muy productivos y, al igual que los acuíferos de caliza, pueden ser vulnerables a la contaminación cuando están expuestos a actividades de uso del suelo. En general, se reconoce que las rocas carbonatadas de Dinamarca están muy fracturadas y cubiertas por espesos sedimentos cuaternarios. El flujo de fisuras es pronunciado, y se produce en los 50Karstic100 m superiores debido a las glaciaciones del Pleistoceno. Según los mapas recientemente publicados sobre la distribución del karst en Europa, Dinamarca no tiene karst. Sin embargo, este estudio concluye que los acuíferos kársticos de creta y caliza son una importante fuente de agua dulce en Dinamarca. Cuatro conjuntos de datos nacionales sobre las características del karst, el flujo de las aguas subterráneas, la química de las aguas subterráneas y los datos sobre el índice de calidad ecológica (EQR) de los peces indican ahora unas estructuras más heterogéneas y unas vías de flujo preferentes en los acuíferos de creta y caliza de lo que se había conceptualizado y modelado con un modelo regional de recursos hídricos de aguas subterráneas/superficiales en el pasado reciente. Este estudio aporta nuevas pruebas cualitativas de que el flujo rápido y preferencial de agua y productos agroquímicos desde la superficie a través de las partes más finas de las capas de cobertura cuaternarias, los sumideros y las fracturas agrandadas por la solución pueden afectar a la vulnerabilidad de los acuíferos de creta y caliza. Además, debido al sistema de flujo preferencial, algunas corrientes de alimentación descargadas por los acuíferos calcáreos y calcáreos karstificados muestran mayores valores de EQR de peces cuando se utilizan los datos de descarga diaria del río observados en lugar de la descarga diaria simulada.
摘要
尽管白垩含水层通常与管道流相关联,但它们是与石灰岩含水层一样的出水量高的地下水系统,当暴露于土地利用活动时,它们很容易受到污染。丹麦碳酸盐岩通常被认为是高度破碎的,并被厚的第四纪沉积物覆盖。由于更新世冰川作用,裂缝流明显,发生在上部50Karstic100 m。根据最近公布的欧洲喀斯特分布图,丹麦没有喀斯特。然而,本研究得出的结论是,岩溶白垩和石灰岩含水层是丹麦淡水的重要来源。四个关于岩溶特征、地下水流、地下水化学和鱼类生态质量比(EQR) 数据的国家数据集现在表明,与国家水资源地下水/最近的地表水模型相比,白垩和石灰岩含水层更显出示异质性结构和优先流动路径。本研究提供了新的定性证据,表明水和农用化学品从地表快速和优先地流过第四纪覆盖层的较薄层、落水洞和溶液扩大的裂缝,可能会影响白垩和石灰岩含水层的脆弱性。此外,由于优先流系统,当使用观察到的河流每日流量数据而不是模拟每日流量时,一些由岩溶白垩和石灰岩含水层排泄的补给型河流显示鱼类 EQR 值增加。
Resumo
Embora os aquíferos calcários não sejam muito frequentemente associados ao fluxo de condutos, são sistemas de águas subterrâneas altamente produtivos e, como aquíferos calcários, podem ser vulneráveis à contaminação quando expostos a atividades de uso da terra. As rochas carbonáticas dinamarquesas são geralmente reconhecidas como altamente fraturadas e cobertas por sedimentos quaternários espessos. O fluxo fissural é pronunciado, ocorrendo nos 50Karstic100 m superiores devido às glaciações do Pleistoceno. De acordo com mapas recentemente publicados da distribuição de carste na Europa, a Dinamarca não possui carste. No entanto, este estudo conclui que os aquíferos de greda e calcário carstificados são uma importante fonte de água doce na Dinamarca. Quatro conjuntos de dados nacionais sobre características cársticas, fluxo de águas subterrâneas, química de águas subterrâneas e dados da razão de qualidade ecológica de peixes (RQE), atualmente, indicam estruturas mais heterogêneas e caminhos de fluxo preferenciais nos aquíferos de greda e calcário do que os que haviam sido conceituados e modelados com recursos hídricos nacionais de águas subterrâneas/ modelo de águas superficiais no passado recente. Este estudo fornece novas evidências qualitativas de que o fluxo rápido e preferencial de água e agroquímicos da superfície através camadas delgadas de cobertura Quaternária, sumidouros e fraturas ampliadas por solução provavelmente podem afetar a vulnerabilidade de aquíferos de greda e calcário. Além disso, devido ao sistema de fluxo preferencial, alguns córregos que recebem descarga dos aquíferos calcários e greda carstificados mostram valores de RQE de peixes aumentados ao usar dados observados de descarga diária do rio em vez de descarga diária simulada.
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Introduction
Around the North Sea in North-West Europe, there is a chalk and limestone basin that occurs in England (UK), France, Belgium, the Netherlands, Germany, Sweden, and Denmark. The onshore margin of this basin is today used for water supply in most of these countries (Downing et al. 1993). Chalk and limestone aquifers supply about one-third of Denmark’s potable water (Vangkilde-Pedersen et al. 2011). Around 15% of the ice-free continental land surface consists of carbonate rocks (Goldscheider et al. 2020), and more than 9% of the world’s population depends on water from karst aquifers (Stevanovic 2019)—for example, groundwater from karst systems in Southern Europe and South-West China contributes 50% or more to regional freshwater supplies (Lu 2007; Hartmann et al. 2014; Chen et al. 2017; Kurkova et al. 2019). Clean and sufficient groundwater from these important aquifers in Denmark and elsewhere contribute to the maintenance of the ecological status in groundwater-dependent ecosystems associated with springs, river valleys, and the river network (Bonnaci et al. 2009) throughout wet and dry seasons in a cold climate (Graeber et al. 2017; Johansen et al. 2018) and warmer climate (Liu et al. 2009). A recent assessment of the quantitative status of Danish groundwater bodies in relation to the European Water Framework Directive River Basin Management Plans has revealed that about six of the 30 large chalk and limestone groundwater bodies are in poor quantitative status due to overexploitation of the aquifers (European Commission 2006; Henriksen et al. 2021a).
The development of karst drainage structures depends on the hydraulic gradient and the flux of carbon dioxide through the karst system (Bakalowicz 2005). Geomorphology and hydrology in karst terrains define the development of characteristic karst features like sinkholes, dolines, karst lakes, karst caves, and disappearing streams (White 1988). Karstified aquifers show some characteristics as a result of dissolution and physiographic features that may favor rapid, turbulent flow from the land surface through subsurface pipe-like voids and conduits that may reach the land surface forming visible springs or less visible wet surfaces near streams (MacDonald et al. 1998; Taylor and Greene 2008; Worthington and Soley 2017). This has implications for the vulnerability of the karstic chalk aquifer and the potential pollution risk of groundwater-dependent ecosystems due to interactions between aquifers, streams, and springs (Kresic and Mikszewski 2013; Goldscheider 2019; Hartmann et al. 2021; Kalvans et al. 2021; Maurice et al. 2021).
Karstic aquifers are often located in the shallow parts of the groundwater system and, hence, are connected to springs and gaining river reaches, thereby securing high quality and often stable ecological baseflow and high flow pulses important for the renewal of macrophytes and fish habitats in the stream network (Yang et al. 2008; Henriksen et al. 2021b; Graeber et al. 2014, 2017). Comprehensive literature describes the relationship between stream flow statistics and the ecological flow regime of macroinvertebrates, macrophytes, and fish around the world (Poff et al. 2010; Poff and Zimmerman 2010; Hoekstra et al. 2012; Westwood et al. 2017). Many EU member states have used ecological flow standards in implementing plans for the assessment of ecological flows in River Basin Management Plans (European Commission 2015; European Environment Agency 2021). Ecological flows were derived as part of the second river basin management plan (2014–2020) and have been implemented again in the third river basin management plan for 2021–2027 for all river basin districts in Denmark (Henriksen et al. 2021a; Liu et al. 2020a, b).
A recent mapping of the distribution of exposed carbonate rock with karst development across Europe by Chen et al. (2017) indicates that Denmark has no karst aquifers (only 0.1% of carbonate rock outcrops). The European karst map by Chen et al. (2017) has used a pragmatic approach where only exposed carbonate rocks with karst development have been included in the hydrogeological map. Karst aquifers in Denmark are covered by thick Quaternary sediments; therefore, they have been overlooked and not recognized as karst aquifers by Danish hydrogeologists and in the international karst community. This is even though karst landscapes and other karst features in chalk and limestone aquifers have been highlighted in the Danish geological literature since the beginning of the 19th century (e.g. Ussing 1899; Jessen 1905). Bækgaard et al. (1982) published a detailed hydrogeological map of the European Union with karstified Upper Cretaceous chalk in central Jylland, which has been incorporated into a map of aquifers across Europe (BGR 2015).
The purpose of this study has been (1) to evaluate the degree of karstification in chalk and limestone deposits in Denmark; (2) to gather the available evidence on rapid (preferential) groundwater flow and karst behavior in groundwater and discharge to gaining streams connected to chalk and limestone aquifers in Denmark using national data on geology, hydrology, geochemistry and ecology, and (3) to discuss to which extent the possible karst development could affect the vulnerability of groundwater bodies and groundwater-dependent ecosystems. It is hypothesized here that, based on new understanding of karst behavior in confined chalk and limestone aquifers in Denmark, one can implement more heterogeneous structures and preferential flow pathways than had been conceptualized and modelled with a national water resources groundwater/surface-water model in the recent past (Henriksen et al. 2003). The need for a more accurate conceptualization is warranted in studies that support policy for protecting river discharge from karstified aquifers resulting in improved conditions for fish and more efficient groundwater source protection planning.
Methods and materials
Research area
Denmark is located in Northern Europe and has an area of about 43,000 km2 (Fig 1a). The average annual precipitation is about 750 mm. The average air temperature is 8–9 °C and is identical to the temperature of Danish groundwater. Several small to larger streams exist; about 85% of Denmark’s streams are connected to aquifers with approximately equal portions in glacial derived sandy and clayey sediments (Sechu et al. 2021) and a small portion (about 100 km) to chalk and limestone sediments. The vast majority of the stream network represents gaining river conditions. Data on the stream network used in this study were created and are available at the Department of Ecoscience, Aarhus University, Denmark. The aquifers with which the stream network is in contact are based on the geological layers extracted from the Danish National Water Resources model (Henriksen et al. 2003) commonly known as the DK model.
a Geological map of the carbonaceous rocks below the Quaternary cover layers in Denmark. The layer units from Upper Cretaceous to Selandian are all carbonaceous rock types. Geological layers in white color are deposited later than Paleocene. Locations with reported karst features are shown on the map. Dashed line box outlines the research area of this study. b Geological cross-section (X1–Y1, located in a) through Djursland where the chalk and limestone deposits gradually become younger from northeast to southwest (modified from Thomsen 1995). c Geological profile in TWT (TwoWayTraveltime, seconds) illustrating the structural elements and presence of thick records of sediments (up to 5 km thick) in the Danish Basin and slightly thinner in the Sorgenfrei-Tornquist Zone (line X2–Y2, a). The blue layer is the Upper Cretaceous chalk and Danian limestone (Chalk Group), the yellowish, greenish, and reddish layers are interbedded sandstones and mudstones, and the purple layer at the base is the Zechstein Salt (modified from Mathiesen et al. 2021)
Denmark is a low-lying land with hills generally less than 150 m high, and the Quaternary cover layers are dominated by glacial deposits from the latest glaciation (Weichselian) (Pedersen 2012). The Quaternary deposits in the research area are dominated by more than 25 m of cover layers and smaller areas with less than 20–25 m thickness, and in a few places chalk and limestone crops out at the ground surface (Fig. 2). Morphological features of the glacial deposits related to karstification of the upper parts of fractured chalk and limestone aquifers have been interpreted and reported in the Danish geological literature (Nilsson and Gravesen 2018). Based on this, a generic geographical information system (GIS) database with information on karst feature types, underlying carbonate lithology, thicknesses of glacial cover layers, and geographical position, was compiled and evaluated. In the research area, sinkholes are distributed over the entire area. In the western part are large karstic lakes (e.g. Vandet lake) located, and in the central part are gaining streams fed by the famous karstic springs in Rold Forest, forming a chalk aquifer–stream system (Berg 1951; Fig. 2).
Evidence of karst features predominantly occurring at locations with less than 15–20 m of glacial-derived sediment cover in central and northern Jylland. Both field-validated karst features and sinkholes based on DEM LiDAR data from Sørensen et al. (2017) are shown
Distribution of the chalk and limestone aquifers and stream system
The chalk and limestone aquifers were deposited during the geological periods of Upper Cretaceous (99.6–65.5 Ma) and Paleocene deposits from Danian and Selandian (65.5–58.7 Ma). The Upper Cretaceous chalk deposition in Denmark occurred in a part of the large North European carbonate sea where Denmark was covered by the sea and consisted of at least 450 m of chalk deposits (Surlyk et al. 2013). The chalk is a fine-grained deposit where more than 80% of the chalk mass has a grain size of less than 5 μm. The chalk in Denmark is a white and yellow-white micrite chalk that is generally soft except for thin yellow hard grounds, occasionally black chert layers, and thin marl layers. The main component is coccoliths but small clay content occurs (Håkansson et al. 1974). The Mid Danian carbonate consists of Bryozoan reef limestone interbedded with chert (Surlyk et al. 2006) and Danian chalk of muddy or micritic chalk mudstone (Blinkenberg et al. 2020). The Late Danian limestone deposits, named København limestone, is classified as a calcarenite and consist of a yellow-white limestone with some thick chert layers and are often a hard massive limestone (Stenestad 1976). The overlying Early Selandian (Lower Paleocene) deposits were laid down after a period of erosion and often begin with glauconitic conglomerate and greensand, named Greensand limestone, followed by a massive light grey silty marl with strong bioturbation and without layering, named Kerteminde Marl (Heilmann-Clausen 1995). The pre-Quaternary unconformity thus truncates the Danian as well as Selandian units (Hallam 1984).
The geological and structural framework of the subsurface throughout the research area can be illustrated with two geological profiles that are constructed in vertical sections through the subsurface. The profiles give an insight into the geological and structural elements of the subsurface, including the position of faults and salt structures as well as the thickness, extent, and continuity of geological layers. Figure 1b shows a cross-section (line X1–Y1 in Fig. 1a) through Djursland with carbonate rocks from Upper Cretaceous to Danian where the chalk and limestone gradually become younger from northeast to southwest. The profile shows the stratigraphical position of the Upper Cretaceous chalk and Danian limestone and chalk layers in the subsurface. Figure 1c is a geological profile showing the large-scale structures through the central and northern Jylland area (line X2–Y2 in Fig. 1a). The section is based on composite seismic profiles forming the background images (Mathiesen et al. 2021). In the section are highlighted layers from the Permian to Cretaceous. The Zechstein Group (Permian) contains thick salt deposits that locally have moved upwards to form salt diapirs or salt pillows in the Cretaceous and Danian sequence of chalk and limestone deposits. The chalk and limestone layers above and in the neighborhood are structural and chemically disturbed by the salt structure, with causes high salt concentrations in the surrounding aquifers. In the water management of the freshwater resource, the chalk and limestone aquifers above the salt structure have been omitted as a groundwater body in the research area (Troldborg 2020).
Databases and interpretation
This paper uses four national data sets to support hypotheses about the presence of overlooked areas with potentially karstified aquifers in Denmark. The datasets include: (1) karst features, (2) groundwater flow based on calculated hydraulic conductivities (K) derived from pumping tests in carbonate wells; (3) water quality based on nitrate concentrations over the drinking water quality standard (50 mg nitrate per liter) in monitoring wells in chalk/limestone and strontium isotopes in surface waters, and (4) systematic bias in fish-based EQR calculations in streams connected to chalk/limestone aquifers.
Pumping test data
A data set of transmissivity data from about 9,500 boreholes solely obtained from pumping tests in fractured chalk and limestone wells was collected from the national well database (JUPITER) of groundwater and water quality (GEUS 2022a). These data of varying quality were calculated from pumping tests of different lengths using water level measurements from both observation and abstraction wells. The hydraulic conductivity (K) has been estimated by dividing the transmissivity value by the length of the open borehole.
The distribution of boreholes with pumping tests performed in chalk and limestone aquifers is presented in Fig. 3. The data are not spread evenly in the chalk and limestone groundwater bodies, which consist of about 30 chalk and limestone aquifers larger than 15 km2 in horizontal extension (Troldborg 2020). The geographical coverage of boreholes with pumping tests is most dense in the Metropolitan area of Copenhagen and Jylland in the center of the chalk and limestone aquifers. Much lower density occurs in the peripheral areas of the chalk and limestone groundwater bodies in most parts of the country (Fig. 3). Data are calculated from pumping tests undertaken in boreholes that were drilled for groundwater abstraction purposes (Kidmose et al. 2022). About 1.5 % of the estimated K values derived from chalk and limestone wells represent fracture/fissure-dominated groundwater flow with a median K value of 1 × 10–4 m/s (<1 × 10–2 and >1 × 10–6 m/s). Approximately 3–4 % of all boreholes have a calculated K value lower than 1 × 10–6 m/s (matrix-dominated flow).
The distribution of boreholes in Denmark with pumping tests in the chalk and limestone aquifers (in total 9,500 tests) as reported to the national borehole database. The boreholes are differentiated according to lithology. Latest delineation of the chalk and limestone aquifers (GWB2020) by Troldborg (2020)
Water quality in connected chalk and limestone aquifer–stream systems
Diffuse nitrate contamination of groundwater from agriculture is causing severe environmental problems in Denmark (Carstensen et al. 2007). National regulations aimed at reducing the leaching of nitrate were successful, reducing the leaching of excess nitrate by approximately 33% on a national level (Kronvang et al. 2008). However, the Himmerland region in the northern part of Jylland (Fig 1a) shows a 31% reduction in nitrate leaching to groundwater, but only 8% of the monitored streams show a downward trend in nitrate concentration (Kronvang et al. 2008). In this area, many karstic springs discharge to the Lindenborg stream valley, where the spring water and some stream water sites were sampled in February 2015 for nitrate measurement; continuous measurements of natural tracers such as temperature and electrical conductivity (EC) in karstic springs have been successfully used to not only provide qualitative information about the karst drainage pattern (Liñán Baena et al. 2009; Luhmann et al. 2011) but also to estimate flow conduit volumes and mixing ratios by studying their time lag to precipitation-induced discharge peaks (Birk et al. 2004; Screaton et al. 2004). In this field study, continuous measurements of EC at 30-min intervals from the Egebaek spring and Lindenborg stream, ~300 m upstream and downstream from the spring discharge to the stream, will be presented to show how the karstic spring and stream in a karstic area react to a precipitation event of 25 mm on 25–26 March 2015.
A national water quality dataset with time series of nitrate data in chalk/limestone aquifers is used for the assessment of the chemical status of the groundwater bodies as part of the third EU river basin management plan (Thorling et al. 2020). For each monitoring point, the mean value of the annual mean of nitrate concentrations for the period 2013–2018 was estimated by Thorling et al. (2020).
The source of water can be traced based on the samples’ strontium (Sr) concentration and strontium isotopic ratio (Wang et al. 2006; Shand et al. 2009). Strontium isotope ratio is a conservative tracer with the 87Sr/86Sr ratio of waters not changing considerably from weathering of minerals until it reaches the surface waters through underground pathways. Anthropogenic Sr sources, for instance, fertilizers, can however change this isotope ratio (Hosono et al. 2007). The geographical distribution of strontium isotopes (87Sr/86Sr) in Danish surface water (streams, springs, and lakes) was studied by Frei and Frei (2011). Frei et al. (2020) found that Sr and strontium isotopes showed a close relationship, on a national scale, between groundwater in pre-Quaternary carbonate sediments and glacial-derived sediments and surface waters (stream, spring, and lakes). In this study strontium isotope compositions have been adopted from Frei and Frei (2011) at 25 surface-water sampling locations in streams, springs, and lakes. All the sampling sites can be related to Quaternary aquifers of glacial derived sediments or chalk and limestone aquifers.
Fish EQR ratio for chalk- and limestone-aquifer-related streams
The approach taken here is to build on the flow–biota relationship between flow regime statistics and fish ecological quality ratio developed by Graeber et al. (2014, 2017), for identifying the ecological flow indicators of the gaining streams potentially connected to karstic aquifers by looking for areas with systematic bias in the calculated simulated fish EQR by comparing results of (Eq. 1) using observed and model-simulated discharge data:
In the empirical Eq. (1), BFI signifies the baseflow index (baseflow volume divided by total flow volume); SIN is the class of stream sinuosity ranging between 1 and 4, where 1 is a straight channel, and 4 is a fully meandering stream calculated from stream length divided by linear distance; Fre25 is the annual frequency of events with flows above the 25th percentile (derived from flow duration curve), and Fre75 is the annual frequency of events with flows below the 75th percentile (derived from the flow duration curve).
It is hypothesized here that a reason for this directed bias (systematic underestimation when using modelled instead of observed daily discharge time series) is preferential flow processes due to immature karstic chalk and limestone aquifer conditions. To the authors’ knowledge, no one has previously used evidence-based empirical equations for fish EQR (Eq. 1) for identifying potential karstic preferential flow dynamics.
For the ecological flow signature, the empirical formula (Eq. 1) for fish-based EQR has been derived for Danish streams (Graeber et al. 2014). The EQR ratio is expressed as a numerical value between 0 and 1, with high ecological status represented by values close to 1 and bad ecological status by values close to 0. The fish EQR is classified by the following thresholds: high (>0.94), good (0.72–0.94), moderate (0.40–0.71), poor (0.11–0.39) and bad (<0.11).
In the following, the simulated difference in fish EQR is used, where Q is flow rate or discharge. The main criteria used are:
The justification for using a threshold of 0.06 is that it is a significant difference (or impact) when compared to changes that one sees for impacts from groundwater abstraction and/or climate change which very rarely for Northern Jylland exceed a change of 0.06 when compared to the reference scenario, e.g. simulation compared to the simulation without abstraction (Liu et al. 2020b). Observed daily discharge time series and calculations of Fre25, Fre75, and BFI for the period 2004–2017 for potential immature karstified chalk and limestone, nonkarstic chalk and limestone, and Quaternary/pre-Quaternary dominated areas has revealed by trial-and-error that a set of supplementary criteria solely based on observed discharge could be made for providing a more robust and data-driven identification of karst areas for central and northern Jylland: Fre25-Fre75 (Qobs) > 2.0, and BFI (Qobs) > 0.80.
The calculations were made by use of a cloud-based ICT tool (VandWeb) for comparing time series of observed groundwater abstractions, and simulated and observed daily flows developed for implementing and surveying ecological flows associated with licenses for groundwater abstraction (GEUS 2022b). VandWeb contains access to observed and simulated discharge data based on a national water resource model (Stisen et al. 2019; Henriksen et al. 2016). Flexible python tools allow calculations of fish, macrophyte, and macroinvertebrate EQRs in specified periods based on empirical equations developed by Graeber et al. (2014).
Results
Geomorphological features
A survey of surface karst features by Nilsson and Gravesen (2018) has been supplemented with recent years of reports of surface karst features from citizens directly to the Geological Survey of Denmark and Greenland or via the media (in total 42 locations). The karst features can be separated into sinkholes that are funnel-shaped (including fluviokarst) or vertical-sided dolines, karst-lakes or sinkhole ponds with subsurface outlets, small caves, karst springs and losing stream sections to chalk and limestone aquifers (here named ‘disappearing streams’). The locations of karst caves, karst landscapes, sinkholes/dolines, and disappearing streams occur across the entire country (Fig. 1a). Karst lakes and karst springs are so far only described for localities in the western part of Denmark.
Sinkholes/dolines have been mapped at three locations using high-resolution LiDAR elevation data in 1.6 and 0.4-m resolution with a vertical accuracy of a few centimeters (SDFE 2022). The regional frequency of these features is rather variable, with the highest density in the Thisted area ranging from 15 to 20 sinkholes/dolines per km2 (Sørensen et al. 2017), and much lower density <1 sinkhole/doline per km2 in the Kolindsund area (Jensen 2021) (Fig. 2). Sinkholes/dolines are primarily found in recharge areas. For comparison, a study in Chalk from southern England counts more than 150 dolines per km2 in high-density areas (MacDonald et al. 1998).
It has been possible to make a characterization of a single sinkhole based on direct excavation of trenches across the sinkhole depression compared with the morphology of the high-resolution LiDAR data (Sørensen et al. 2017). It seems many circular depressions in the soil surface near Thisted and Kolindsund probably have an origin as cover-collapse sinkholes (Panno and Luman 2018).
Based on the present karst feature data, there is a relationship between locations with karst springs, lakes, and karst landscapes developed in Upper Cretaceous chalk, and disappearing streams above Bryozoan limestone, while sinkholes and caves develop in Upper Cretaceous chalk and Danian chalk and limestone (Fig. 1a). The majority of the karst features are located with less than 20–25-m thickness of Quaternary glacial derived sediments (Fig. 2). The observations (as part of this study) on findings of sinkholes in terrain at places with less than 20 m of glacial-derived sediments agree with North American studies in glacial terrains east of the Rocky Mountains where karst features (especially sinkholes) are predominantly observed in areas with a maximum of 15–20 m thickness of glacial derived sediments (Soller and Packard 1998; Weary and Doctor 2014).
High hydraulic conductivities in fault zones
The carbonate deposits contain tectonic faults and fractures. The faults are mainly vertical, while the fractures and conduits often follow bedding planes above or below chert and marl layers. The fractures and conduits are stained with yellow-brown Fe components from water flow (Rosenbom and Jakobsen 2005). Figure 4 shows Mesozoic fault zones that have been developed during the geologic periods Trias, Jura, and Cretaceous. It should be noted that the Mesozoic fault zones shown do not necessarily all cut the chalk layers in the Upper Cretaceous (See Fig. 1c).
Distribution of fault zones in Denmark and the boreholes with extremely high hydraulic conductivity values determined by pumping tests. Mesozoic fault zones are shown (from GEUS (2022a)). The grey-shaded area is the latest delineation of chalk and limestone aquifers (chalk and limestone GWB2020, adapted from Troldborg 2020)
The uppermost approximately 50–100 m of chalk and limestone have been disturbed by the Quaternary glaciers’ large-scale glaciotectonic thrusting and folding. Isolated bodies of chalk or limestone are often included in the Quaternary layers. The erosion of the surface has also formed shallow depressions which have been further eroded by meltwater streams or depressions left in the land surface when ice, formerly covered by sediment, melts and kettle holes develop (Gry 1979). In an area south of Ålborg, the Skrivekridt is locally exposed and has a fractured aquifer that shows a combination of karst processes and fluvial erosion and deposition known as fluviokarst (Stenestad 2006).
Extraordinary high hydraulic conductivity (K) values represent K values above 1× 10–2 m/s or more than 864 m/day, that are assumed to be reliable and represent hydraulic conditions in the chalk and limestone with pronounced fracture systems or fault zones with rapid non-Darcian (turbulent) groundwater flow (Marechal et al. 2008). The extreme high K values make up a ratio of 1.5% or a total of ~125 pumping tests. There appears to be a correlation between the boreholes in Upper Cretaceous chalk and the Mesozoic fault zones (Fig. 4). In the eastern part of the country, extreme high K values are primarily associated with the Greensand limestone and København limestone in the Metropolitan area of Copenhagen. These two carbonate rock types are characterized by a facies-controlled strong local-scale horizontal fissure development between hard and softer horizons in the two units (Jakobsen and Klitten 1999), that have probably been expanded further over time by chemical dissolution (karstification). High K values in Bryozoan limestone in the Copenhagen area are likely to be explained by local-scale glaciotectonic fractures. In the western part of the country, high K values occur evenly distributed between the carbonate rock types of København limestone, Bryozoan limestone, and Upper Cretaceous chalk. This kind of geological structure is prone to infiltration of surface water into the surface and discharge of groundwater from aquifers to gaining parts of surface waters.
Water quality in the aquifer–stream system
Nitrate groundwater quality
High nitrate fertilizer concentrations (>50 mg nitrate/L) in chalk/limestone monitoring wells (red circles) are shown in Fig. 5 as average concentrations for the period of 2013–2018. There is a close relationship found between the location of monitoring wells with nitrate concentrations above 50 mg/L in chalk and limestone aquifers and cover thickness of up to 15–20 m.
Water quality in the aquifer–stream system in chalk and limestone in central and northern Jylland. Monitoring wells in chalk and limestone aquifers (red dots) are shown with nitrate concentrations above 50 mg/L determined as annual mean values from 2013 to 2018. Strontium isotope ratio in surface waters indicates that the Sr source is groundwater originating from chalk/limestone aquifers (<0.7082) and glacial sediment aquifers (>0.7082) in accordance to Frei and Frei (2011). Streams connected to Quaternary (ks1–ks4) aquifers and chalk and limestone aquifers are also given. The locations with the discharge of groundwater originating from chalk/limestone discharge into lakes (location No. 1: Vandet lake), into springs (location No. 3: Trindborg spring), and into gaining stream sections of Dybvad Stream (location No. 2) and Villestrup Stream at two sampling locations (location No. 4)
Strontium in groundwater, streams, springs, and lakes
In accordance with Frei and Frei (2011) strontium isotope compositions of surface water connected to chalk and limestone aquifers range from 0.7078 to 0.7082 in the central and northern Jylland. Surface water (streams, springs, lakes) in the research area connected to Quaternary aquifers (ks1–ks4) has strontium compositions larger than 0.7082. Strontium isotope composition data from Frei and Frei (2011) are shown in Fig. 5. Strontium isotope composition indicates that Sr isotopes are an excellent tracer to distinguish between Sr isotope samples collected in surface waters connected to chalk and limestone aquifers or Quaternary aquifers.
Water quality relations in the chalk aquifer–stream system
Nitrate concentrations measured in seven karstic springs in February 2015 reveal that the four springs discharging on the left bank of Lindenborg stream have elevated nitrate concentrations of 40–47 mg/L, while the three springs on the right bank show concentrations between 0.5 and 15 mg/L (Fig. 6). This distinction between the left and right stream valley can be attributed to land-use practices where the left bank of the stream valley is mainly used for agricultural production, while the right bank is a forested area. Nitrate concentrations in the stream also reflect the influence of spring water; nitrate concentration just upstream of the Egebaek spring is 16.8 mg/L, while a few hundred meters downstream this rises to 19.7 mg/L as a consequence of spring discharge with high nitrate concentrations to Lindenborg Stream.
Nitrate concentrations in karstic springs and streams in the Lindenborg Stream valley. The map also shows the location of the Egebaek spring and the upstream and downstream stations in the Lindenborg Stream where electrical conductivity (EC) was continuously monitored. The karst springs Kovadsbaek, Ravnkilde, and Lille Blaakilde were also investigated seven decades ago with measured discharge rates of 80–150 L/s by Berg (1951)
The reaction of the karstic Egebaek spring and Lidenborg Stream to a rain event on 25–26 March 2015 is presented by continuous EC measurements (Fig. 7). Before the rain event, both the upstream and downstream stream stations show a stable EC of 0.49 and 0.51 mS/cm, respectively, while EC in the Egebaek spring varies between 0.52 and 0.61 mS/cm. During the rain event, EC in Lindenborg Stream quickly decreases to 0.35 mS/cm both at the upstream and downstream stream station reaching a minimum value approximately at the end of the precipitation event. EC data from the Egebaek spring, however, does not reflect such a change and displays the previous variations in EC until 30 March 2015 with a time lag of 5 days after the start of the rain event. The decrease in EC is also much longer than for the stream. At both stream stations, EC values almost reach the original stable values 1 day after the event, while the karstic spring only returns to pre-event values 10 days after the onset of EC decrease.
Electrical conductivity (EC) of spring water at the Egebaek spring and ~300 m upstream and downstream of the location where the spring discharges into the Lindenborg Stream (for the location refer to Fig. 6). The vertical black lines denote the duration of the rain event on 25–26 March 2015. EC values are recalculated to a reference temperature of 25 °C
Fish EQR index and flow dynamics in chalk streams
The stream network within the area with chalk and limestone aquifers is shown in Fig. 8. Those streams of the carbonate groundwater bodies where the carbonate surface is less than 3m below the stream network in the national water resource model are shown with bold red lines (Troldborg 2020). It is assumed that there is potential hydraulic contact between the streams and underlying chalk and limestone aquifers on these stream stretches so that the stream can receive discharging groundwater through the stream bed. Catchments, where fish EQR calculated from observed discharge are more than 0.06 higher than fish EQR calculated from simulated discharge, are delineated in Fig. 8 (marked with ‘Eflow’). Here the hypothesis is that observed discharge in karstic chalk areas has more pronounced peak flow fluctuation events (Fre25 above Q25) compared to nonkarstic chalk conditions, and at the same time without having an equally increased number of low flow fluctuation events (Fre75 below Q75). Karstic conditions at the time do not significantly impact modelled values compared to observed discharge calculated BFI.
The entire stream network is connected to chalk/limestone (red line) and Quaternary aquifers (ks1 and ks4) in central and northern Jylland. Areas with systematic bias (underestimation) in fish ecological quality ratio (EQR) for simulated daily flow, compared to observed flow, are marked with ‘Eflow’ on the map where it is likely that the streams are connected to karstified aquifers or streams connected to Quaternary aquifers (ks1–ks4), where layer ks1 is most shallow and layer ks4 has a deeper lithostratigraphical location. Streams labelled 1–19 refer to stream numbers in Table 1
Selected gauging stations for calculated Δ fish EQR (Qobs, Qsim), Fre25-Fre75 (Qobs), and BFI (Qobs) with karstic and nonkarstic properties are shown in Table 1. Since the fish EQR Eq. (1) is developed for larger stream catchments (>10 km2) the selected results are mainly from larger streams (and not small tributaries/headwaters).
Figure 9 shows that the eco-hydrological status for the selected 19 streams in the research area can be classified into three grades (good, moderate and poor) based on the fish-based EQR index. Due to the recharge from the karst groundwater to the related streams, especially in the dry season, the specific low flow of karstic streams is generally larger than that of nonkarst streams. In Fig. 8, karstic streams are clustered towards the right side along the horizontal axis, and this would suggest that the ecological status of karstic streams is better.
In general the identified nine karstic stream catchments comply with all three criteria (Δ fish EQR > 0.06, Fre25-Fre75 > 2 and BFI > 0.8). Overall, these streams and catchments have a good-to-moderate ecological status when EQR is calculated based on Eq. (1) (the empirical relationship). For the other streams, one or more of the three criteria have not been met either because of BFI < 0.80, Fre25-Fre75 ≤ 2, or Δ fish EQR ≤ 0.06. If one only used the Δ fish EQR > 0.06 criteria then a total of four ‘false positive’ gauging stations would have been identified with karstic conditions out of a total of 19 gauging stations (i.e. 21% false positive). If one used only BFI and Fre25-Fre75 as criteria without using a comprehensive physically based groundwater/surface-water model, only one false positive gauging station (i.e. 5% false positive) would have been identified. This means that the approach for central and northern Jylland is more robust. For the nonkarstic catchments, the calculated ecological status is typically moderate with one exception of ‘Land Fjord Canal’ (see Table 1), which gets the ecological status ‘bad’. In general, the nine catchments with karstic streams are connected to chalk and limestone aquifers as expected, and two to lithostratigraphic deeper-lying Quaternary aquifers (ks3 and ks4). The 10 nonkarstic streams are predominantly ‘without contact’ or gaining streams in contact with the shallow Quaternary aquifer types ks1 and ks2; however, there are also examples of stream sections in contact with chalk and limestone aquifers for this group.
Discussion
In Paleocene time when the Danian and Selandian carbonates were deposited, significant and severe changes in sea level appeared. Thomsen (1995) interpreted up to four phases with very low sea level or even dry conditions with an outcropping of the chalk and limestone surface due to uplift and erosion. The paleo-geographical and climatic conditions indicate that there may have been the right conditions for the formation of karst in the surface of the chalk and limestone deposits in Northern Europe before the end of the Paleocene time (Downing et al. 1993). Northern Europe was located ~900 km further south than now so the Danish area was at a latitude of about 45 °N (Ziegler 1990). The climate was constantly warmer (around 20 °C) with arid climate conditions (Hancock 1975). For parts of the chalk and limestone surfacing in the Danish land area at that time, the conditions for the development of karst at the top of the limestone deposits were therefore ideal. Seismic surveys of the chalk and limestone surface (Top Chalk surface) under the North Sea suggest that karst topography was subaerially exposed in the North Sea in mid-Paleocene time (Jenyon 1987; Huuse 1999). The present karstification of the on-shore fractured chalk and limestone aquifers with fracture systems to a depth of 50–100 m has most likely been developed after the end of the most recent ice age 11,700 years before the present, allowing ample time for subsequent expansion of fissure openings by chemical dissolving processes of chalk and limestone materials along the fissures with the help of circulating groundwater. However, it cannot be ruled out that the karst formation already started during earlier interglacial/interstadial periods in the Holocene period. In addition, it cannot be excluded that some of the larger fault systems were formed in the late Mesozoic (Upper Cretaceous). However, regardless of the time of formation, these fault zones will also likely be exposed to circulating groundwater since the end of the last ice age, with karstification as a result.
Thus far, limited observations have been made of field-verified karst features on the land surface like sinkholes, springs, and disappearing streams in Denmark. In the subsurface, only very few caves are known. There may be doubts as to whether they are carbonate rock mines established 400 years ago when Denmark was a major exporter of flint material to neighboring countries. There is, as far as the authors are aware, no knowledge of underground drainage conduits dominated by rapid groundwater flow. However, it should be mentioned that many geophysical video logs in open carbonate rock abstraction boreholes show turbulent groundwater flow in fracture zones. Facies and structural studies using geophysical methods compared with cored borehole material are valuable to explore karst system conduits and fracture systems (Chalikakis et al. 2011; Chatelier et al. 2011).
Contamination of chalk aquifer groundwater with fertilizers (Foster 2000) and pesticides (Malaguerra et al. 2012) has been known since the 1960s. The hydraulic connectivity between the fractured chalk aquifer and the land surface is closely related to the thickness of the confining layer and geological heterogeneity. Chalk aquifers are most vulnerable to groundwater pollution where the cover layers are thin or absent. In areas where the thickness of the glacial-derived sediments over the chalk in Denmark is less than 15–20 m, the chalk aquifers are categorized as very vulnerable. A similar assessment of chalk aquifer vulnerability is reported from the US where chalk or potentially karstified chalk aquifers superimposed with thinner glacial deposits (less than about 15 m) are categorized as very vulnerable (Weary and Doctor 2014). The geological and geochemical heterogeneity in glacial-derived sediments can be described by dividing the cover layer into conceptual submodels, each with an indexed vulnerability to pollution transport using the depth development of fractures and sand lenses, and analysis of complex redox structures, as a proxy for the spread of pollution vertically (Klint et al. 2013; Hansen et al. 2021). Even minor heterogeneities with high hydraulic conductivity may form preferential flow paths and dominate flow and transport through the otherwise impermeable clayey till cover layers (Sidle et al. 1998; Harrington et al. 2007; Harrar et al. 2007; Kessler et al. 2013).
Adams et al. (2016) point out that the absence of karst features on the land surface does not imply the absence of karst processes in the subsurface. It has been speculated that small and more simple sinkholes might indicate relatively small conduits in underlying carbonate bedrocks and larger structures might be situated close to underlying cave systems. Intensive forestry and agricultural operations within karst landscapes can more or less erase these features (Sørensen et al. 2017; Panno and Luman 2018; Hofierka et al. 2018).
Karstic preferential flow catchments show a tendency for more significant systematic bias in calculated fish EQR based on empirical Eq. (1), with an underestimated fish EQR value for simulated discharge compared to observed discharge mainly due to a more narrow Q75-Q25 band and underestimation of Fre25 for karstic streams compared to nonkarstic. At the same time, identified karstic catchments for observed discharge in central and northern Jylland have Fre25-Fre75 > 2.0 and BFI > 0.80 and good to moderate ecological status. Fish EQR, as pointed out by Liu et al. (2020a), is positively related to BFI and negatively related to Fre75, underlining the importance of a stable discharge regime with a generally rare occurrence of low flow events. The positive relation to Fre25 with more frequent slight disturbances due to weak peak flows (exceeding Q25) reflects an improved quality of the fish habitats and community. Since karstic preferential flow conditions support both dynamics, e.g. higher frequency of Fre25 compared to other aquifers and increased baseflow through conduit voids, such stream conditions are in general favorable for complying with a good status of such streams. Since Fre25-Fre75 is also part of the macrophyte indicator, which in addition has a duration of three times Q25 flow events, good status for aquatic plants is often also obtained for karstic streams. Finally, since also Q90/Q50 typically are above 0.5 for such streams, karstic streams also score good status if the physical/morphological index is in good status.
Other studies on the base flow index in karst areas in Indonesia, Bulgaria, and the UK are in good agreement with the chosen BFI > 0.8 in this study. An Indonesian study has estimated BFI values for karst springs up to 0.9 (Fatchurohman et al. 2018). Vasileva et al. (2017) used a BFI of 0.8 for Bulgarian karst systems, while the UK baseflow index is set at 0.9 for chalk (UK_CEH 2022)—for example, in the Pang and Lambourn river catchments in southern England, the BFI indexes were calculated to be 0.87 and 0.96, respectively (Maurice et al. 2006; Maurice 2009). However, the research reported here shows that the criteria limits that distinguish karst streams from nonkarst streams are not universal and can likely not be transferred to other parts of Denmark or abroad. Chalk and limestone streams for Sjælland in the eastern part of Denmark do not fulfill these supplementary criteria, so either the chalk and limestone on Sjælland are to a less degree karstic than in central and northern Jylland, or other stressors (urbanization and groundwater abstraction) impact the low flows (BFI and Fre75), requiring specific analysis of usable thresholds for Sjælland and the eastern islands.
The authors believe that it is therefore the overall Δ fish EQR > 0.06 criteria, to a high degree defined by the difference in Fre25 and Fre75 between observed and simulated flow (and driven by pulses of rapid precipitation impacts), that may be transferable to Sjælland and Fyn, even though BFI and low flows are almost zero in dry summers in this part of Denmark. However, a validation based on proxy basin tests of the developed methodology for Sjælland and Fyn, and other areas in Northern Europe and abroad, is needed, and as part of that, more universal supplementary criteria eventually need to be identified.
Conclusion and perspectives
Although the chalk aquifers are not too often associated with conduit flow, they are highly productive groundwater systems, like limestone aquifers, and can be highly vulnerable to contamination when exposed to land use activities. This study shows that karstified chalk and limestone aquifers are important freshwater resources in Denmark. The occurrence of karst aquifers underneath confining cover layers of glacial-derived sediments is more widespread than hitherto recognized and new to the international karst community. In an updated version of the world karst map, Denmark and probably other parts of the world must be included in the map as a new category of karst aquifers. Evidence of rapid groundwater flow due to the presence of karstified chalk and limestone aquifers in Denmark has been evaluated using four selected indicators based on national data on geology, hydrogeology, geochemistry, and ecological flow indicators. Surface karst features indicate more heterogeneous structures and preferential pathways to the chalk and limestone aquifers than conceptualized at present in Denmark. Overall, the chalk and limestone appear to be gently karstic with local-scale karst development. Identification and implementation of appropriate measures to protect the aquifers require a proper conceptualization and modelling of the freshwater cycle in the chalk and limestone aquifers. A better understanding of the morphology, distribution, and evolution of the various karst features in the land surface is required to provide insights into the character of the underlying carbonate geology and karst aquifers in Denmark. The presented ecological flow ratio for fish has so far only been investigated for karst conditions in central and northern Jylland, Denmark. However, it must be considered quite probable that in areas elsewhere, a fish EQR equation can be established based on regression, and that the methodology described here can inspire criteria for ecological flow for other areas. It is concluded that, to the authors’ knowledge, this is the first time that fish EQR empirical formulas have been used to evaluate possible karstic conditions based on an analysis of runoff time series in streams.
References
Adams R, Barry J, Green J (2016) Minnesota regions prone to surface karst feature development. Series GW-01, Minnesota Department of Natural Resources, Ecological and Water Resources Division, St. Paul, MN. https://files.dnr.state.mn.us/waters/groundwater_section/mapping/gw/gw01_report.pdf. Accessed November 2022
Bækgaard A, Kelstrup N, Andersen LJ, Krøyer S (1982) Groundwater resources, 1: aquifers – sheet 3. In: Commission of European Communities, Directorate-General Information Market and Innovation, Brussels, Luxembourg (map)
Bakalowicz M (2005) Karst groundwater: a challenge for new resources. J Hydrol 13:148–160. https://doi.org/10.1007/s10040-004-0402-9
Berg K (1951) Notes on some large Danish springs. Hydrobiologia 3:72–78
BGR (2015) European groundwater map IHME1500. BGR - Groundwater - European Groundwater Map IHME1500 available as vector data. https://www.bgr.bund.de/EN/Themen/Wasser/Aktuelles/Archiv/2015-04_ihme1500_vektordaten_en.html. Accessed 18 October 2022
Birk S, Liedl R, Sauter M (2004) Identification of localized recharge and conduit flow by combined analysis of hydraulic and physico-chemical spring responses (Urenbrunnen, SW-Germany). J Hydrol 286:179–193. https://doi.org/10.1016/j.jhydrol.2003.09.007
Blinkenberg KH, Lauridsen BW, Knaust D, Stemmerik L (2020) New ichnofabrics of the Cenomanian-Danian Chalk Group. J Sediment Res 90:701–712. https://doi.org/10.2110/jsr.2020.37
Bonacci O, Pipan T, Culver DC (2009) A framework for karst ecohydrology. Environ Geol 56:891–900. https://doi.org/10.1007/s00254-008-1189-0
Carstensen J, Henriksen P, Heiskanen A (2007) Summer algal blooms in shallow estuaries: definition, mechanisms, and link to eutrophication. Limnol Oceanogr 52(1):370–384. https://doi.org/10.4319/lo.2007.52.1.0370
Chalikakis K, Plagnes V, Guerin R, Valois R, Bosch FP (2011) Contribution of geophysical methods to karst-system exploration: an overview. Hydrogeol J 19:1169–1180. https://doi.org/10.1007/s10040-011-0746-x
Chatelier M, Ruelleu S, Bour O, Porel G, Delay F (2011) Combined fluid temperature and flow logging for characterization of hydraulic structure in a fractured karst aquifer. J Hydrol 400:377–386. https://doi.org/10.1016/j.jhydrol.2011.01.051
Chen Z, Auler AS, Bakalowicz M, Drew D, Griger F, Hartmann J, Jiang G, Moosdorf N, Richts A, Stevanovic Z, Veni G, Goldscheider N (2017) The World Karst Aquifer Mapping project: concept, mapping procedure and map of Europe. Hydrogeol J 25:771–785. https://doi.org/10.1007/s10040-016-1519-3
Downing RA, Price M, Jones GP (1993) The making of an aquifer. In: Downing RA, Price M, Jones GP (eds) The hydrogeology of the chalk of North-West Europe. Oxford University Press, Oxford, UK, pp 1–13
European Commission (2006) European Commission. Directive 2000/60/EC of the European Parliament and of the Council, of 23 October 2000, establishing a framework for community action in the field of water policy. Off J Eur Communities 2000 327:1–73
European Commission (2015) Ecological flows in the implementation of the Water Framework Directive: guidance document no. 31. EC, Brussels. https://doi.org/10.2779/775712
European Environment Agency (2021) Water resources across Europa: confronting water stress—an updated assessment. EEA report no. 12 2021. https://doi.org/10.2800/320975
Fatchuroman H, Adji TN, Wijayanti P (2018) Baseflow index assessment and master recession curve analysis for karst water management in Kakap Spring, Gunung Sewu. Earth Environ Sci 148, art. no. 012029. https://doi.org/10.1088/1755-1315/148/1/012029
Foster SSD (2000) Assessing and controlling the impacts of agriculture on groundwater: from Barley Barons to Beef Bans. Q J Eng Geol Hydrogeol 33(4):263–280. https://doi.org/10.1144/qjegh.33.4.263
Frei KM, Frei R (2011) The geographic distribution of strontium isotopes in Danish surface waters: a base for provenance studies in archaeology, hydrology and agriculture. Appl Geochem 26:326–340. https://doi.org/10.1016/j.apgeochem.2010.12.006
Frei R, Frei KM, Kristiansen SM, Jessen S, Schuellehner J, Hansen B (2020) The link between surface water and groundwater-based drinking water: strontium isotope spatial distribution patterns and their relationships to Danish sediments. Appl Geochem 121, https://doi.org/10.1016/j.apgeochem.2020.104698
GEUS (2022a) National well database (JUPITER). https://eng.geus.dk/products-services-facilities/data-and-maps/national-well-database-jupiter. Accessed 2 February 2022
GEUS (2022b) National water resources model for Denmark. Web-based ICT screening is used by the municipalities for groundwater abstraction permissions. https://vandmodel.dk/vandweb. Python scripts for fish EQR calculation from time series are available from PyPI. https://pypi.org/project/hydroinform. Accessed 7 June 2022
Goldscheider N (2019) A holistic approach to groundwater protection and ecosystem services in karst terrains. Carbonates Evaporites 34:1241–1249. https://doi.org/10.1007/s13146-019-00492-5
Goldscheider N, Chen Z, Auler AS, Bakalowicz M, Broda S, Drew D, Hartmann J, Jiang GH, Moosdorf N, Stevanovic Z, Veni G (2020) Global distribution of carbonate rocks and karst water resources. Hydrogeol J 28:1661–1677. https://doi.org/10.1007/s10040-020-02139-5
Graeber D, Wiberg-Larsen P, Bøgestrand J, Baastrup-Pedersen A (2014) Vurdering af effekten af vandindvinding på vandløbs økologiske tilstand [Assessment of the effect of water extraction on the ecological condition of streams]. Notat fra DCE - Nationalt Center for Miljø og Energi, Aarhus, The Netherlands, pp 1–29
Graeber D, Jensen TM, Rasmussen JJ, Riis T, Wiberg-larsen P, Baattrup-Pedersen A (2017) Multiple stress response of lowland stream benthic macroinvertebrates depends on habitat type. Sci Total Environ 599–600:1517–1523. https://doi.org/10.1016/j.scitotenv.2017.05.102
Gry H (1979) Description of the geological map of Denmark: map sheet Løgstør—Quaternary deposits (in Danish). Danmarks Geologiske Undersøgelse I Række, no. 26, The Geological Survey of Denmark and Greenland, Copenhagen, 58 pp
Håkansson E, Bromley RG, Perch-Nilesen K (1974) Maastrichtian Chalk of Northwest Europe: a pelagic shelf sediment. In: Hsü JJ, Jenkyns HC (eds) Pelagic sediments on land and under the sea. Spec. Publ. Int. Sediment., vol 1, Blackwell, London, pp 211–234
Hallam A (1984) Pre-Quaternary sea-level changes. Annu Rev Earth Planet Sci 12:205–243. https://doi.org/10.1146/annurev.ea.12.050184.001225
Hancock JM (1975) The sequence of facies in the Upper Cretaceous of northern Europe compared with that in the western interior. In: Caldwell CWG (ed) The Cretaceous system in the western interior of North America. Special papers of the Geological Association of Canada, no. 13., pp 83–118, Geological Association of Canada, Ottawa
Hansen B, Voutchkova D, Sandersen PBE, Kallesoe A, Thorling L, Moller I, Madsen RB, Jakobsen R, Aamand J, Maurya P, Kim H (2021) Assessment of complex subsurface redox structures for sustainable development of agriculture and the environment. Environ Res Lett 16(2). https://doi.org/10.1088/1748-9326/abda6d
Harrar WG, Murdoch LC, Nilsson B, Klint KES (2007) Field characterization of vertical bromide transport in a fractured glacial till. Hydrogeol J 15(8):1473–1488. https://doi.org/10.1007/s10040-007-0198-5
Harrington GA, Hendry MJ, Robinson N (2007) Impact of permeable conduits on solute transport in aquitards: mathematical models and their application. Water Resour Res 43(5). https://doi.org/10.1029/2005WR004144
Hartmann A, Goldscheider N, Wagener T, Lange J, Weiler M (2014) Karst water resources in a changing world: review of hydrological modeling approaches. Rev Geophys 52:218–242. https://doi.org/10.1002/2013RG000443
Hartmann A, Jasechko S, Gleeson T, et al (2021) Risk of groundwater contamination widely underestimated because of fast flow into aquifers. Proc Natl Acad Sci USA 118 (20). https://doi.org/10.1073/pnas.2024492118
Heilmann-Clausen C (1995) Palæogene aflejringer over Danskekalken [Paleogene deposits above the Danish chalk], chap 5. In: Nielsen OB (ed) Danmarks geologi fra kridt til i dag [Denmark’s geology from Cretaceous to the present day]. Geologisk Institut, Aarhus Universitet, Aarhus, The Netherlands, pp 69–114
Henriksen HJ, Troldborg L, Nyegaard P, Sonnenborg TO, Refsgaard JC, Madsen B (2003) Methodology for construction, calibration and validation of a national hydrological model for Denmark. J Hydrol 280:52–71. https://doi.org/10.1016/S0022-1694(03)00186-0
Henriksen HJ, Stisen S, van der Keur P, Danapour M, He X, Troldborg L (2016) Operationalisering af ny viden til administration af indvindingstilladelser Operationalization of new knowledge for administration of extraction permits. GEUS report 2016/46, GEUS, Copenhagen. https://doi.org/10.22008/gpub/32467
Henriksen HJ, Voutchkova D, Ondracek M, Troldborg L, Thorling L (2021a) Konsolidering af kvantitativ tilstandsvurdering for danske grundvandsforekomster i potentiel ringe tilstand på basis af ekspertvurdering. Consolidation of quantitative conditions assessment for Danish groundwater bodies in potentially poor condition on the basis of expert assessment. GEUS report 2021/2, GEUS, Copenhagen. https://doi.org/10.22008/gpub/34562
Henriksen HJ, Jakobsen A, Pasten-Zapata E, Troldborg L, Sonnenborg TO (2021b) Assessing the impacts of climate change on hydrological regimes and fish EQR in two Danish catchments. J Hydrol-Regional Stud 34:100798. https://doi.org/10.1016/j.ejrh.2021.100798
Hoekstra AY, Mekonnen MM, Chapagain AK, Mathews RE, Richter BD (2012) Global monthly water scarcity: blue water footprints versus blue water availability. PLoS One 7(2):e32688. https://doi.org/10.1371/journal.pone.0032688
Hofierka J, Gallay M, Bandura P, Sasak J (2018) Identification of karst sinkholes in a forested karst landscape using airborne laser scanning data and water flow analysis. Geomorphology 308:265–277. https://doi.org/10.1016/j.geomorph.2018.02.004
Hosono T, Nakano T, Igeta A, Tayasu I, Yachi (2007) Impact of fertilizer on a small watershed of Lake Biwa: use of sulfur and strontium isotopes in environmental diagnosis. Sci Total Environ 384:342–354. https://doi.org/10.1016/j.scitotenv.2007.05.033
Huuse M (1999) Detailed morphology of the Top Chalk surface in the eastern Danish North Sea. Pet Geosci 5:303–314. https://doi.org/10.1144/petgeo.5.3.303
Jakobsen PR, Klitten K (1999) Fracture systems and groundwater flow in the København Limestone Formation. Nord Hydrol 30:301–316
Jessen A (1905) The geological map of Denmark in scale 1:100.000 with descriptions: map sheet Aalborg and Nibe (northern part) (in Danish). Danmarks Geologiske Undersøgelse I Række. Geological Survey of Denmark and Greenland, Copenhagen, 10:193 maps
Jensen JM (2021) Investigation of possible karstification and transient groundwater modelling of Grenå catchment. MSc Thesis, Copenhagen University, Denmark pp 108
Jenyon MK (1987) Seismic expression of real and apparent buried topography. J Pet Geol 10:41–48. https://doi.org/10.1111/j.1747-5457.1987.tb00995.x
Johansen OM, Andersen DK, Ejrnaes R, Pedersen ML (2018) Relations between vegetation and water level in groundwater dependent terrestrial ecosystems (GWDTEs). Limnologica 68:130–141. https://doi.org/10.1016/j.limno.2017.01.010
Kalvans A, Popovs K, Priede A, Koit O, Retike I, Bikse J, Delina A, Babre A (2021) Nitrate vulnerability of karst aquifers and associated groundwater-dependent ecosystems in the Baltic region. Environ Earth Sci 80(18). https://doi.org/10.1007/s12665-021-09918-7
Kessler TC, Comunian A, Oriani F, Renard P, Nilsson B, Klint KE, Bjerg PL (2013) Modeling fine-scale geological heterogeneity: examples of sand lenses in tills. Groundwater 51(5):692–705. https://doi.org/10.1111/j.1745-6584.2012.01015.x
Kidmose J, Sonnenborg TO, Henriksen HJ, Nilsson B (2022) Effective porosity and hydraulic conductivity in fractured chalk and limestone aquifers. GEUS report 2022/13, GEUS, Copenhagen. https://doi.org/10.22008/gpub/34648
Klint KE, Nilsson B, Troldborg L, Jakobsen P (2013) A poly morphological landform approach for hydrogeological applications in heterogeneous glacial sediments. Hydrogeol J 21:1247–1264. https://doi.org/10.1007/s10040-013-1011-2
Kresic N, Mikszewski A (2013) Hydrogeological conceptual site models: data and visualisation. CRC, Boca Raton, FL
Kronvang B, Andersen HE, Børgesen C, Dalgaard T, Larsen SE, Bøgestrand J, Blicher-Mathiesen G (2008) Effects of policy measures implemented in Denmark on nitrogen pollution of the aquatic environment. Environ Sci Pol 11:144–152. https://doi.org/10.1016/j.envsci.2007.10.007
Kurkova I, Bruthans J, Balak F, Slavik M, Schweigstillova J, Bruthansova J, Mikus P, Vojtisek J, Grundloch J (2019) Factors controlling evolution of karst conduits in sandy limestone and calcareous sandstone (Turnov area, Czech Republic). J Hydrol 574:1062–1073. https://doi.org/10.1016/j.jhydrol.2019.05.013
Liñán Baena C, Andreo B, Mudry J, Carrasco Cantos F (2009) Groundwater temperature and electrical conductivity as tools to characterize flow patterns in carbonate aquifers: the Sierra de las Nieves karst aquifer, southern Spain. Hydrogeol J 17:843–853. https://doi.org/10.1007/s10040-008-0395-x
Liu C, Lang Y, Li S, Piao H, Tu C, Liu T, Zhang W, Zhu S (2009) Researches on biogeochemical processes and nutrient cycling in karstic ecological systems, southwest China: a review (in Chinese with English abstract). Earth Sci Front 16:1–12
Liu W, Bailey RT, Andersen HE, Jeppesen E, Park S, Todsen H, Nielsen A, Molina-Navarro E, Trolle D (2020a) Assessing the impacts of groundwater abstractions on flow regime and stream biota: combining SWAT-MODFLOW with flow-biota empirical models. Sci Total Environ 745. https://doi.org/10.1016/j.scitotenv.2020.140933
Liu W, Bailey RT, Andersen HE, Jeppesen E, Nielsen A, Peng K, Molina-Navarro E, Park S, Thodsen H, Trolle D (2020b) Quantifying the effects of climate change on hydrological regime and stream biota in a groundwater-dominated catchment: a modelling approach combining SWAT MODFLOW with flow-biota empirical models. Sci Total Environ (745). https://doi.org/10.1016/j.scitotenv.2020.140933
Lu Y (2007) Karst water resources and geo-ecology in typical regions of China. Environ Geol 51:695–699. https://doi.org/10.1007/s00254-006-0381-3
Luhmann AJ, Covington MD, Peters AJ, Alexander SC, Anger CT, Green JA, Runkel AC, Alexander EC Jr (2011) Classification of thermal patterns at karst springs and cave streams. Groundwater 49(3):324–335. https://doi.org/10.1111/j.1745-6584.2010.00737.x
MacDonald AM, Brewerton LJ, Allen DJ (1998) Evidence for rapid groundwater flow and karst-type behavior in the Chalk of southern England. In: Robins NS (eds) Groundwater pollution, aquifer recharge and vulnerability. Geol Soc Lond Spec Publ 130:95–106
Malaguerra F, Albrechtsen H-J, Thorling L, Binning PJ (2012) Pesticides in water supply wells in Zealand, Denmark: a statistical analysis. Sci Total Environ 414:433–444. https://doi.org/10.1016/j.scitotenv.2011.09.071
Marechal J-C, Ladouche B, Dörfliger N (2008) Interpretation of pumping tests in a mixed flow karst system. Water Resour Res 44:5. https://doi.org/10.1029/2007WR006288
Mathiesen A, Midtgaard HH, Hjelm L (2021) Studies of geological properties and conditions for deep disposal of radioactive waste, Denmark. Phase 1, report no. 6. Subsurface distribution of Jurassic and Cretaceous fine-grained formations based on seismic mapping. GEUS report, vol 2021, no. 57, GEUS, Copenhagen. https://doi.org/10.22008/gpub/34617
Maurice L (2009) Investigations of rapid groundwater flow and karst in the Chalk. PhD Thesis, University College London, UK
Maurice L, Atkinson TC, Barker JA, Bloomfield JP, Farrant AR, Williams AT (2006) Karstic behavior of groundwater in the English Chalk. J Hydrol 330:63–70. https://doi.org/10.1016/j.jhydrol.2006.04.012
Maurice L, Farrant AR, Mathewson E, Atkinson T (2021) Karst hydrogeology of the chalk and implications for groundwater protection. Geol Soc Lond Spec Publ 517. https://doi.org/10.1144/SP517-2020-267
Nilsson B, Gravesen P (2018) Karst geology and regional hydrogeology in Denmark. In: White WB, Herman JS, Herman EK, Rutigliano M (eds) Karst groundwater contamination and public health. Advances in Karst Science. Book series. Springer, Cham, pp 289–298. https://doi.org/10.1007/978-3-319-51070-5_34
Panno SV, Luman DE (2018) Characterization of cover-collapse sinkhole morphology on a groundwater basin-wide scale using LiDAR elevation data: a new conceptual model for sinkhole evolution. Geomorphology 318:1–17. https://doi.org/10.1016/j.geomorph.2018.05.013
Pedersen SAS (2012) Glaciodynamic sequence stratigraphy. In: Huuse M, Redfern J, Le Heron DP, Dixon RJ, Moscariello A, Craig J (eds) Glaciogenic reservoirs and hydrocarbon systems. Geol Soc Lond Spec Publ 368:29–51
Poff NL, Zimmerman JKH (2010) Ecological responses to altered flow regimes: a literature review to inform the science and management of environmental flows. Freshw Biol 55:194–205. https://doi.org/10.1111/j.1365-2427.2009.02272.x
Poff NL, Richter BD, Arthington AH, Bunn SE, Naiman RJ, Kendy E, Acreman M, Apse C, Bledsoe BP, Freeman MC, Henriksen J, Jacobson RB, Kennen JG, Merritt DM, O’Keeffe JH, Olden JD, Rogers K, Tharme RE, Warner A (2010) The ecological limits of hydrologic alteration (ELHOHA): a new framework for developing regional environmental flow standards. Freshw Biol 55:147–170. https://doi.org/10.1111/j.1365-2427.2009.02204.x
Rosenbom AE, Jakobsen PR (2005) Infrared thermography and fracture analysis of preferential flow in chalk. Vadose Zone J 4:271–280. https://doi.org/10.2136/vzj2004.0074
Screaton E, Martin JB, Ginn B, Smith L (2004) Conduit properties and karstification in the unconfined Floridan aquifer. Groundwater 42(3):338–346. https://doi.org/10.1111/j.1745-6584.2004.tb02682.x
SDFE (2022) The Danish digital elevation model provided by the Danish Agency for Data Supply and Efficiency. www.sdfe.dk. Accessed 5 July 2022
Sechu G, Nilsson B, Iversen BV, Greve MB, Børgesen CD, Greve MH (2021) A stepwise GIS Aapproach for the delineation of river valley bottom within drainage basins using a cost distance accumulation analysis. Water 13:827. https://doi.org/10.3390/w13060827
Shand P, Darbyshire DPF, Love AJ, Edmunds WM (2009) Sr isotopes in natural waters: applications to source characterization and water–rock interaction in contrasting landscapes. Appl Geochem 24:574–586. https://doi.org/10.1016/j.apgeochem.2008.12.011
Sidle RC, Nilsson B, Hansen M, Fredericia J (1998) Spatially varying hydraulic and solute transport characteristics of a fractured till determined by field tracer tests, Funen, Denmark. Water Resour Res 34(10):2515–2527. https://doi.org/10.1029/98WR01735
Soller DR and Packard PH (1998) Digital representation of a map showing the thickness and Quaternary sediments in the glaciated United States east of the Rocky Mountains. US Geol Surv Data Ser DDS-38, scale 1:1,000,000
Sørensen PB, Lykke-Andersen H, Gravesen P, Nilsson B (2017) Karst sinkhole mapping using GIS and digital terrain models. Geol Surv Denmark Greenland Bull 38:25–28
Stenestad E (1976) Københavnsområdets geologi især baseret på citybaneundersøgelserne [The geology of the Copenhagen area, especially based on the city rail surveys]. Danmarks Geologiske Undersøgelse III rk, no. 45, Geological Survey of Denmark and Greenland, Copenhagen, 149 pp
Stenestad E (2006) Fluviokarst in the top of the Maastrichtian chalk at Rørdal, northern Jutland, Denmark. Bull Geol Soc Denmark 53:93–110
Stevanovic Z (2019) Karst waters in potable water supply: a global scale overview. Environ Earth Sci 78. https://doi.org/10.1007/s12665-019-8670-9
Stisen S, Ondracek M, Troldborg L, Schneider RJM, van Til MJ (2019) National vandressource model: modelopstilling og kalibrering af DK-model 2019 (The national water resource model. Model setup and calibration of DK model 2019). GEUS report 2019/31, GEUS, Copenhagen. https://doi.org/10.22008/gpub/32631
Surlyk F, Damholt T, Bjerager M (2006) Stevns Klint Formation: Uppermost Maastrichtian chalk, Cretaceous-Tertiary boundary, and lower Danian Bryozoan mound complex. Bull Geol Soc Denmark 54:1–46
Surlyk F, Rasmussen SL, Boussaha M, Schiøler P, Schovsbo NH, Sheldon E, Stemmerik L, Thibault N (2013) Upper Campanian – Maastrichtian holostratigraphy of eastern Danish basin. Cretac Res 46:232–256. https://doi.org/10.1016/j.cretres.2013.08.006
Taylor CJ, Greene EA (2008) Hydrogeologic characterization and methods used in the investigation of karst hydrology, chap 3. In: Rosenberry DO, LaBaugh JW (eds) Field techniques for estimating water fluxes between surface water and ground water. US Geol Surv Tech Methods 4-D2, pp 71–114
Thomsen E (1995) Kalk og kridt i den danske undergrund [Limestone and chalk in the Danish underground]. In: Nielsen OB (ed) Danmarks geologi fra Kridt til i dag [Denmark’s geology from Cretaceous to the present day]. Aarhus Univ Geokompendier 1:32–67
Thorling LS, Nilsson B, Møller I, Troldborg L, Sandersen P (2020) Metode for vurdering af de danske grundvandsforekomsters kemiske tilstand og nye vurderinger af tilstanden fsva. nitrat [Method for assessing the chemical state of the Danish groundwater bodies and new assessments of the state of nitrate]. GEUS report 2020/4, GEUS, Copenhagen
Troldborg L (2020) Delineation of the Danish groundwater bodies (in Danish). GEUS report 2020/1. https://www.geus.dk/Media/2/5/GEUSrapport_2020_1_GVF_afgraensning_web.pdf. Accessed November 2022
UK_CEH (2022) Derived flow statistics. National River Flow Archive. UK Centre for Ecology and Hydrology. https://nrfa.ceh.ac.uk/derived-flow-statistics. Accessed 6 June 2022
Ussing NV (1899) Danmarks geologi i almindeligt omrids [Denmark’s geology in general outline]. Danmarks Geologiske Undersøgelse III rk., no. 2, 264 pp, Geological Survey of Denmark and Greenland, Copenhagen
Vangkilde-Pedersen T, Mielby S, Jakobsen PR, Hansen B, Holst Iversen C, Nielsen AM (2011) Kortlægning af kalkmagasiner [Mapping of limestone and chalk aquifers]. Geo-Vejledning 8, GEUS, Copenhagen, 108 pp
Vasileva T, Orehova T, Kroumova K (2017) Groundwater contribution to the river flow for the upper course of the Osam River based on two baseflow separation methods. Geol Balcan 46(2):73–82
Wang Y, Guo Q, Su C, Ma T (2006) Strontium isotope characterization and major ion geochemistry of karst water flow, Shentou, northern China. J Hydrol 328:592–603. https://doi.org/10.1016/j.jhydrol.2006.01.006
Weary DJ, Doctor DH (2014) Karst in the United States: a digital map compilation and database. US Geol Surv Open-File Rep 2014-1156
Westwood CG, England J, Dunbar MJ, Holmes NTH, Leeming DJ (2017) An approach to setting ecological flow thresholds for southern English chalk streams. Water Environ J 31:528–536. https://doi.org/10.1111/wej.12275
White WB (1988) Geomorphology and hydrology of karst terrains. Oxford University Press, New York, 464 pp
Worthington SRH, Soley RWN (2017) Identifying turbulent flow in carbonate aquifers. J Hydrol 552:70–80. https://doi.org/10.1016/j.jhydrol.2017.06.045
Yang YCE, Cai XM, Herricks EE (2008) Identification of hydrologic indicators related to fish diversity and abundance: a data mining approach for fish community analysis. Water Resour Res 44(4):W04412. https://doi.org/10.1029/2006WR005764
Ziegler PA (1990) Geological atlas of Western and Central Europe. Shell Internationale Petroleum, The Hague, The Netherlands
Acknowledgements
Valuable comments and suggestions by associate editor Joseph Mas-Pla and two anonymous reviewers are much appreciated and have significantly improved the manuscript.
Funding
This study was supported by the European Union project ‘Rural Water and Food Security (PI RURAL) which has initiated knowledge sharing between EU member states and China on karst hydrology and protection and restoration of groundwater-dependent ecosystems (GDE). Results from the project ‘Effective porosity and hydraulic conductivity in chalk and limestone aquifers’ funded by the Danish Environmental Protection Agency are also included. The National Science Foundation of China (contract No. 41572242) ‘Eco-hydrological Characteristics of the Karst Groundwater-dependent Ecosystems (KGDEs) and its Developing Conditions’ has also funded the paper writing.
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Nilsson, B., Li, F., Chen, H. et al. Evidence of karstification in chalk and limestone aquifers connected with stream systems and possible relation with the fish ecological quality ratio in Denmark. Hydrogeol J 31, 53–70 (2023). https://doi.org/10.1007/s10040-022-02565-7
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DOI: https://doi.org/10.1007/s10040-022-02565-7