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Fixation of polyvinylidene fluoride (PVDF) mesh with cyanoacrylate-derived glues in a rat experimental model: histopathologic immunohistochemical and morphometric study

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A Correction to this article was published on 05 February 2020

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Abstract

Purpose

The use of cyanoacrylate (CA)-based tissue adhesives for mesh fixation in abdominal hernia repair is increasing due to the fast action and bond strength of these glues. The aim of the present study was to assess tissue changes induced by different CA glues used for mesh fixation in an animal model.

Methods

Parietal defects were induced in the abdominal wall of 60 rats and repaired by polyvinylidene fluoride (PVDF) mesh fixation using different CA glues. At 1, 7, 15, and 30 days post-surgery, macroscopic and histopathological studies were performed to evaluate mesh adhesion, the presence of complications and the tissue response.

Results

All meshes were successfully fixed without signs of inflammatory reaction, displacement or detachment. In areas where CA adhesives were applied, the acute tissue response was limited and transient. At 7 days post-surgery, collagen fibril production around prosthetic materials was observed, and collagen maturation was achieved at 30 days post-surgery. Good mesh incorporation was detected with all three glues, but the application of Glubran-2 was associated with an early macrophagic response and the early production and maturation of collagen fibrils.

Conclusions

Our study confirmed that CA tissue adhesives induced the good incorporation of prosthetic mesh within host tissue with a low incidence of complications and reduced acute tissue reaction. At 30 days post-surgery no signs of mesh disinsertion or migration were observed, the prosthetic mesh adhesion was due to the presence of a dense mature connective tissue rich in type I collagen fibres.

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Change history

  • 05 February 2020

    Unfortunately, during the revision of the manuscript the title has been incorrectly published

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Funding

Restricted grant provided by GEM Srl for data analysis.

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Authors and Affiliations

  1. Dipartimento Di Scienze Veterinarie, Università Di Pisa, Viale delle Piagge, 2, 56124, Pisa, Italy

    A. Poli, F. Parisi, F. Millanta & V. Miragliotta

  2. Dipartimento Di Medicina Clinica E Sperimentale, Università Di Pisa, Pisa, Italy

    L. Solfanelli

  3. Abdominal Wall Surgery Unit, Surgery Department, La Fe University and Politechnic Hospital, Valencia, Spain

    P. García-Pastor

  4. Centro Di Ricerca “E. Piaggio”, Università Di Pisa, Pisa, Italy

    C. Magliaro

  5. Fondazione Toscana “Gabriele Monasterio” E Centro Di Biomedicina Sperimentale del CNR, Pisa, Italy

    S. Burchielli

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  1. A. Poli
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Correspondence to A. Poli.

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The authors declare no conflicts of interest.

Human and animal rights

All procedures performed in studies involving animals were in accordance with the international ethical standards. This article does not contain any studies with human participants performed by any of the authors.

Ethical approval

Permission to conduct this experimental study (Authorization n. 755/2015-PR) was granted by the Italian Ministry of Health, in accordance with Italian Laws DL.gs 26/2014 and Directive 2010/63/EU on the protection of animals used for scientific purposes.

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No informed consent was necessary for the study.

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Poli, A., Parisi, F., Millanta, F. et al. Fixation of polyvinylidene fluoride (PVDF) mesh with cyanoacrylate-derived glues in a rat experimental model: histopathologic immunohistochemical and morphometric study. Hernia 24, 1263–1273 (2020). https://doi.org/10.1007/s10029-019-02078-5

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