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Remodeling characteristics and biomechanical properties of a crosslinked versus a non-crosslinked porcine dermis scaffolds in a porcine model of ventral hernia repair

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Abstract

Purpose

The objective of this study was to evaluate the histologic remodeling profile and biomechanical properties of the porcine abdominal wall after repair with HDMI-crosslinked (Permacol®) or non-crosslinked (Strattice®) porcine dermis in a porcine model of ventral hernia repair.

Methods

Bilateral incisional hernias were created in Yucatan minipigs and repaired after 21 days. The repair site, including mesh and abdominal wall, was harvested after 1, 6, and 12 months and subjected to histologic analysis and uniaxial testing. Native abdominal wall without mesh was also subjected to uniaxial tensile testing.

Results

Permacol® demonstrated significant improvement over time in every remodeling category except scaffold degradation, while remodeling characteristics of Strattice® remained relatively unchanged over time for every category except fibrous encapsulation and neovascularization. However, remodeling scores for Strattice® were already significantly higher after just 1 month compared to Permacol® in the categories of cellular infiltration, ECM deposition, and neovascularization, providing evidence of earlier remodeling of the non-crosslinked grafts compared to the crosslinked grafts. The tensile strength and stiffness of both crosslinked and non-crosslinked graft-tissue composites were greater than the tensile strength and stiffness of the native porcine abdominal wall in the very early post-operative period (1 month), but there was no difference in tensile strength or stiffness by the end of the study period (12 months).

Conclusions

HDMI collagen crosslinking of porcine dermis scaffolds reduces the early histologic remodeling profile but does not significantly impact the tensile strength or stiffness of the graft-tissue composites in a porcine model of ventral hernia repair.

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Acknowledgments

This research was supported by a grant from Synovis Surgical Innovations, Inc., St. Paul, MN. This data were presented at Hernia Repair 2011, American Hernia Society, San Francisco, California, March 18, 2011. Funding for this project was provided by Synovis Surgical Innovations, Inc. (St. Paul, MN).

Conflict of interest

B.D.M. declares having received funds in relation with this study.

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Authors and Affiliations

  1. Department of Surgery, Section of Minimally Invasive Surgery, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8109, St. Louis, MO, 63110, USA

    J. A. Cavallo, M. M. Frisella, C. R. Deeken & B. D. Matthews

  2. Division of Comparative Medicine, Washington University School of Medicine, St. Louis, MO, USA

    S. C. Greco

  3. Division of Biostatistics, Washington University School of Medicine, St. Louis, MO, USA

    J. Liu

Authors
  1. J. A. Cavallo
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  2. S. C. Greco
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  3. J. Liu
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  4. M. M. Frisella
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  5. C. R. Deeken
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  6. B. D. Matthews
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Corresponding author

Correspondence to C. R. Deeken.

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Cavallo, J.A., Greco, S.C., Liu, J. et al. Remodeling characteristics and biomechanical properties of a crosslinked versus a non-crosslinked porcine dermis scaffolds in a porcine model of ventral hernia repair. Hernia 19, 207–218 (2015). https://doi.org/10.1007/s10029-013-1070-2

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  • DOI: https://doi.org/10.1007/s10029-013-1070-2

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