Abstract
Langerin is a recently identified lectin for which antibodies can be used as immunohistochemical markers of Langerhans cells (LCs). We describe the distribution of staining in autopsy pediatric tissues, dermatopathic and other reactive lymph nodes, and childhood histiocytic lesions using the 12D6 antibody (Novocastra). We also correlate CD1a (antibody O1O) staining to these factors. Langerin on epidermal LCs has a coarsely granular cell membrane and a cytoplasmic staining pattern that is always associated with CD1a expression. All 6 skin samples had Langerin+/CD1a+ LCs within the epidermis. Six of 8 thymuses showed single scattered dendritic-shaped cells in the medulla and rare cells within Hassall corpuscles that coexpressed Langerin and CD1a. Cortical thymocytes were CD1a+/Langerin−. Four of 8 livers examined showed a sinusoidal lining pattern of Langerin+/CD1a−. All 15 autopsy lymph nodes showed a similarly strong Langerin+/CD1a− sinus pattern of staining on fixed tissue elements, mostly in medullary sinuses. All 12 dermatopathic lymph nodes showed accumulation of Langerin+/CD1a+ cells in the pale paracortical nodules. All 24 instances of LC histiocytosis (LCH) were Langerin+/CD1a+. All 12 non-LCH histiocytic disorders are negative for Langerin in the histiocytes of interest. We conclude that Langerin is coexpressed with CD1a on LCs and LCH. Lymph node sinuses and hepatic sinusoids show Langerin+/CD1a− cells, indicating that, when used alone to confirm LCH infiltration, the 12D6 antibody should be used with caution. At other sites, its diagnostic accuracy is similar to that of CD1a.
Similar content being viewed by others
References
J Valladeau V Duvert-Frances J Pin et al. (1999) ArticleTitleThe monoclonal antibody DCGM4 recognizes Langerin, a protein of Langerhans cells and is rapidly internalized from the cell surface Eur J Immunol 29 2695–2704
J Valladeau O Ravel C Dezutter-Dambuyant et al. (2000) ArticleTitleLangerin, a novel C-type lectin specific to Langerhans cells, is an endocytic receptor that induces the formation of Birbeck granules Immunity 12 71–81 Occurrence Handle10.1016/S1074-7613(00)80160-0 Occurrence Handle1:CAS:528:DC%2BD3cXhtVWjtb0%3D Occurrence Handle10661407
S Seguier A Bodineau G Godeau B Pellat N Brousse (2003) ArticleTitleLangerin+ versus CD1a+ Langerhans cells in human gingival tissue: a comparative and quantitative immunohistochemical study Arch Oral Biol 48 255–262 Occurrence Handle10.1016/S0003-9969(02)00173-5 Occurrence Handle1:CAS:528:DC%2BD3sXit1KnsrY%3D Occurrence Handle12663070
M Luftl A Feng E Licha G Schuler (2002) ArticleTitleDendritic cells and apoptosis in mycosis fungoides Br J Dermatol 147 1171–1179 Occurrence Handle10.1046/j.1365-2133.2002.04994.x Occurrence Handle1:CAS:528:DC%2BD3sXksV2ntQ%3D%3D Occurrence Handle12452867
F Geissmann MC Dieu-Nosjean C Dezutter et al. (2002) ArticleTitleAccumulation of immature Langerhans cells in human lymph nodes draining chronically inflamed skin J Exp Med 196 417–430 Occurrence Handle10.1084/jem.20020018 Occurrence Handle1:CAS:528:DC%2BD38XmsV2htLo%3D Occurrence Handle12186835
W Vermi R Bonecchi F Facchetti et al. (2003) ArticleTitleRecruitment of immature plasmacytoid dendritic cells (plasmacytoid monocytes) and myeloid dendritic cells in primary cutaneous melanomas J Pathol 200 255–268 Occurrence Handle10.1002/path.1344 Occurrence Handle12754747
D Bell P Chomarat D Broyles et al. (1999) ArticleTitleIn breast carcinoma tissue, immature dendritic cells reside within the tumor, whereas mature dendritic cells are located in peritumoral areas J Exp Med 190 1417–1426 Occurrence Handle10.1084/jem.190.10.1417 Occurrence Handle1:CAS:528:DyaK1MXnsVams7g%3D Occurrence Handle10562317
T Chu R Jaffe (1994) ArticleTitleThe normal Langerhans cell and the LCH cell Br J Cancer 23 IssueIDsuppl S4–S10 Occurrence Handle1:STN:280:ByuA2MritFc%3D
C Nezelof F Basset (1998) ArticleTitleLangerhans cell histiocytosis research. Past, present and future Hematol Oncol Clin North Am 12 385–406 Occurrence Handle1:STN:280:DyaK1c3it1Ohsw%3D%3D Occurrence Handle9561908
R Jaffe D DeVaughn E Langhoff (1998) ArticleTitleFascin and the differential diagnosis of childhood histiocytic lesions Pediatr Dev Pathol 1 216–221 Occurrence Handle10.1007/s100249900029 Occurrence Handle1:STN:280:DyaK1Mzpt1GktQ%3D%3D Occurrence Handle10463281
R Jaffe (1999) ArticleTitleThe histiocytoses Clin Lab Med 19 135–155 Occurrence Handle1:STN:280:DyaK1MzjtFGisg%3D%3D Occurrence Handle10403078
V Ornvold E Ralfkiaer H Carstensen (1990) ArticleTitleImmunohistochemical study of the abnormal cells in Langerhans cell histiocytosis (histiocytosis X) Virchows Arch A Pathol Anat Histopathol 416 403–410 Occurrence Handle1:STN:280:By%2BC1MvntlE%3D Occurrence Handle2107627
M Kashihara M Ueda Y Horiguchi et al. (1986) ArticleTitleA monoclonal antibody specifically reactive to human Langerhans cells J Invest Dermatol 87 602–607 Occurrence Handle10.1111/1523-1747.ep12455849 Occurrence Handle1:CAS:528:DyaL2sXjs1Sh Occurrence Handle3534103
K Wolff (1967) ArticleTitleThe fine structure of the Langerhans cell granule J Cell Biol 35 468–473 Occurrence Handle10.1083/jcb.35.2.468 Occurrence Handle1:STN:280:CCeD2c%2FjsFQ%3D Occurrence Handle6055996
BE Favara AC Feller M Pauli et al. (1997) ArticleTitleContemporary classification of histiocytic disorders Med Pediatr Oncol 29 157–166 Occurrence Handle10.1002/(SICI)1096-911X(199709)29:3<157::AID-MPO1>3.0.CO;2-C Occurrence Handle1:STN:280:ByiA2MnktVc%3D Occurrence Handle9212839
L Weiss T Dura T Grogan (2001) Histocytic and dendritic cell neoplasms ES Jaffe NL Harris H Stein JW Vardiman (Eds) WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues IARC Press Lyon 280–282
J Valladeau C Dezutter-Dambuyant S Saeland (2003) ArticleTitleLangerin/CD207 sheds light on formation of Birbeck granules and their possible function in Langerhans cells Immunol Res 28 93–107
N Suzuki K Yamamoto S Toyoshima T Osawa T Irimura (1996) ArticleTitleMolecular cloning and expression of cDNA encoding human macrophage C-type lectin. Its unique carbohydrate binding specificity for Tn antigen J Immunol 156 128–135
M Spiess HF Lodish (1985) ArticleTitleSequence of a second human asialoglycoprotein receptor: conservation of two receptor genes during evolution Proc Natl Acad Sci USA 82 6465–6469
F Geissmann Y Lepelletier S Fraitag et al. (2001) ArticleTitleDifferentiation of Langerhans cells in Langerhans cell histiocytosis Blood 97 1241–1248 Occurrence Handle10.1182/blood.V97.5.1241 Occurrence Handle1:CAS:528:DC%2BD3MXhs12ltrk%3D Occurrence Handle11222366
K Shilo W Chu S Abbondanzo T Franks W Travis (2004) ArticleTitleDiagnostic utility of Langerin (CD207) for histological diagnosis of pulmonary Langerhans cell histiocytosis Mod Pathol 17 IssueIDsuppl 1 344A
ACKNOWLEDGMENTS
This study was supported by the Marjory K. Harmer Memorial Endowment. We thank Connie Riga for technical assistance in the preparation of this work.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chikwava, K., Jaffe, R. Langerin (CD207) Staining in Normal Pediatric Tissues, Reactive Lymph Nodes, and Childhood Histiocytic Disorders. Pediatr. Dev. Pathol. 7, 607–614 (2004). https://doi.org/10.1007/s10024-004-3027-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10024-004-3027-z