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Jorge Lobo’s Disease: Immunohistochemical Characterization of Dendritic Cells in Cutaneous Lesions

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Abstract

Background

Jorge Lobo’s disease (JLD) is a cutaneous chronic mycosis caused by Lacazia loboi. We studied Factor XIIIa + dermal dendrocytes (FXIIIa + DD), Langerhans cells (LC) through the expression of langerin and the expression of S100 protein.

Methods

A total of 41 biopsies and 10 normal skins (control) were developed with a polymer-based immunohistochemical method.

Results

Lesions presented infiltrate comprising macrophages, some asteroid corpuscles, lymphocytes, multinucleated giant cells and a large number of fungi. LCs presented short dendrites and were scarcely distributed. Dermal langerin + cells were detected in nine JLD lesions. FXIIIa + DD were hypertrophic, visualized in the inflammatory infiltrate of JLD lesions. Cells S100+ were present in JLD and control group with a similar number of cells. A total of 14 specimens did not express FXIIIa, and this considerable number probably contributed to the statistical similarity with the control group.

Conclusions

The results indicate that LCs are present in the immune response against Lacazia loboi. Some dermal langerin + cells could be another subset of dendritic cells. Our data indicate changes of LCs in JLD cutaneous lesions and present, for the first time, results that show langerin + cells in the dermis and corroborate previous observations on the participation of FXIIIa + DD in the in situ immune response in JLD.

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Acknowledgments

This work was supported by CNPq, Grant 470512/2013-0. We thank Wellington Luiz F. da Silva, BSc, for the excellent technical support.

Conflict of Interest

None of the authors have any potential conflict of interest or financial support in the subject matter.

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Authors and Affiliations

  1. Instituto de Assistência Médica ao Servidor Público Estadual, Programa de pós-graduação em Ciências da Saúde, São Paulo, SP, Brazil

    Tania Cristina Barboza & Carla Pagliari

  2. Laboratório da Disciplina de Patologia de Moléstias Transmissíveis, Departamento de Patologia, Faculdade de Medicina, Universidade de São Paulo, Av. Dr. Arnaldo, 455, sala 1118, Cerqueira Cesar, São Paulo, SP, Brazil

    Tania Cristina Barboza, Maria Irma Seixas Duarte, Mirian Nacagami Sotto & Carla Pagliari

  3. Núcleo de Medicina Tropical, Universidade Federal do Pará, Belém, PA, Brazil

    Juarez Antonio Simões Quaresma, Arival Cardoso de Brito, Marilia Brasil Xavier, Clivia Maria Moraes de Oliveira & Deborah Aben Athar Unger

  4. Centro de Ciências Biológicas e da Saúde, Universidade do Estado do Pará, Belém, PA, Brazil

    Juarez Antonio Simões Quaresma & Marilia Brasil Xavier

Authors
  1. Tania Cristina Barboza
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  2. Juarez Antonio Simões Quaresma
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  3. Arival Cardoso de Brito
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  4. Marilia Brasil Xavier
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  5. Clivia Maria Moraes de Oliveira
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  6. Deborah Aben Athar Unger
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  7. Maria Irma Seixas Duarte
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  8. Mirian Nacagami Sotto
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  9. Carla Pagliari
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Corresponding author

Correspondence to Carla Pagliari.

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Barboza, T.C., Quaresma, J.A.S., de Brito, A.C. et al. Jorge Lobo’s Disease: Immunohistochemical Characterization of Dendritic Cells in Cutaneous Lesions. Mycopathologia 179, 269–274 (2015). https://doi.org/10.1007/s11046-014-9836-3

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  • DOI: https://doi.org/10.1007/s11046-014-9836-3

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