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The combination of extraction and chronocoulometry of a solid precipitate for teaching a novel approach of electroanalysis

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Abstract

In this study, we introduce an innovative approach to electroanalysis that combines solvent extraction and chronocoulometry for the quantification of furosemide, a loop diuretic medication. The objective of this report is to provide students with a practical learning experience in the field of electroanalysis, enabling them to explore recent developments both theoretically and in the laboratory. The experimental procedure involves the extraction of furosemide from a pharmaceutical tablet using methanol, followed by the deposition of the extracted compound onto an electrode. Chronocoulometry is then employed to determine the quantity of furosemide. A 95% recovery rate and a close to 99% fit between theoretical and experimental substance amounts demonstrate the applicability of the method. This approach offers several didactic benefits for students: (i) It teaches the theory and application of chronocoulometry. (ii) It emphasizes the advantage of chronocoulometry as a calibration-free technique. (iii) It introduces students to the principles of electrochemical stripping techniques. (iv) It demonstrates that solid particles can be effectively studied using electroanalysis. Finally, (v) it provides a real-world application of electroanalysis in quantifying an organic compound.

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  • 08 January 2024

    Springer Nature’s version of this paper was updated: Author biographies and photos has been adjusted.

References

  1. Domínguez I, Doménech-Carbó A (2015) Screening and authentication of tea varietiesbased onmicroextraction-assisted voltammetry of microparticles. Sens Act B 210:491–499. https://doi.org/10.1016/j.snb.2015.01.009

    Article  CAS  Google Scholar 

  2. Doménech-Carbó A, Ibars AM, Prieto-Mossi J, Estrelles E, Scholz F, Cebrián-Torrejóna MM (2015) Electrochemistry-based chemotaxonomy in plants using the voltammetry of microparticles methodology. New J Chem 39:7421–7428. https://doi.org/10.1039/C5NJ01233C

    Article  CAS  Google Scholar 

  3. Ortiz-Miranda AS, König P, Kahlert H, Scholz F, Osete-Cortina L, Doménech-Carbó MT, Doménech-Carbó A (2016) Voltammetric analysis of Pinus needles with physiological, phylogenetic, and forensic applications. Anal Bioanal Chem 408:4943–4952. https://doi.org/10.1007/s00216-016-9588-7

    Article  CAS  PubMed  Google Scholar 

  4. Muñiz-Calvo S, Guillamón JM, Domínguez I, Doménech-Carbó A (2017) Detecting and monitoring the production of melatonin and other related indole compounds in different saccharomyces strains by solid-state electrochemical techniques. Food Anal Methods 10:1408–1418. https://doi.org/10.1007/s12161-016-0699-8

    Article  Google Scholar 

  5. Domingos da Silveira G, Di Turo F, Dias D, Fracassi da Silva JA (2020) Electrochemical analysis of organic compounds in solid-state: applications of voltammetry of immobilized microparticles in bioanalysis and cultural heritage science. J Solid State Electrochem 24:2633–2652. https://doi.org/10.1007/s10008-020-04720-0

    Article  CAS  Google Scholar 

  6. Gulppi MA, Vejar N, Tamayo L, Azocar MI, Vera C, Silva C, Zagal JH, Scholz F, Páez MA (2014) Stripping voltammetry microprobe (SPV): a new approach in electroanalysis. Electrochem Commun 41:8–11. https://doi.org/10.1016/j.elecom.2014.01.012

    Article  CAS  Google Scholar 

  7. Gulppi M, Pavez J, Zagal JH, Sancy M, Azocar M, Scholz F, Páez MA (2015) Stripping voltammetry microprobe (SPV): substantial improvements of the protocol. J Electroanal Chem 745:61–65. https://doi.org/10.1016/j.jelechem.2015.03.008

    Article  CAS  Google Scholar 

  8. Scholz F, Meyer B (1998) Voltammetry of solid microparticles immobilized on electrode surfaces. Electroanal Chem 20

  9. Scholz F, Schroeder U, Gulaboski R, Antonio DC (2015) Electrochemistry of immobilized particles and droplets 2nd Edition, Experiments with three phase electrodes. https://eprints.ugd.edu.mk/id/eprint/12083

  10. Doménech-Carbó A, Labuda J, Scholz F (2012) Electroanalytical chemistry for the analysis of solids: characterization and classification (IUPAC Technical Report). Pure Appl Chem 85(3):609–631. https://doi.org/10.1351/PAC-REP-11-11-13

    Article  CAS  Google Scholar 

  11. Tang N, Wang X, Du W, Zhang L, Xiang J, Wang S, Cheng P, Zhu L, Yin Q (2019) Conformational flexibility and crystallization: the case of furosemide. Crystal Growth Design 19:2050–2059. https://doi.org/10.1021/acs.cgd.8b01407

    Article  CAS  Google Scholar 

  12. Kahlert H, Scholz F (2013) Acid-Base Diagrams Springer Berlin 978-3-642-37901-7 (Print) 978-3-642-37902-4 (eBook). https://doi.org/10.1007/978-3-642-37902-4

  13. Baka E, Comer JEA, Takács-Novák K (2008) Study of equilibrium solubility measurement by saturation shake-flask method using hydrochlorothiazide as model compound. J Pharm Biomed Anal 46:335–341. https://doi.org/10.1016/j.jpba.2007.10.030

    Article  CAS  PubMed  Google Scholar 

  14. Bukkitgar SD, Shetti NP (2016) Electrochemical oxidation of loop diuretic furosemide in aqueous acid medium and its analytical application. Cogent Chem 2(1):1152784. https://doi.org/10.1016/j.ejps.2009.04.009

  15. Malode SJ, Abbar JC, Shetti NP, Nandibewoor ST (2012) Voltammetric oxidation and determination of loop diuretic furosemide at a multi-walled carbon nanotubes paste electrode. Electrochim Acta 60:95–101. https://doi.org/10.1016/j.electacta.2011.11.011

    Article  CAS  Google Scholar 

  16. Shetti NP, Sampangi LV, Hegde RN, Nandibewoor ST (2009) Electrochemical oxidation of loop diuretic furosemide at gold electrode and its analytical applications. Intl J Electrochem Sc 4:104–121, http://www.electrochemsci.org/papers/vol4/4010104.pdf

  17. Goss CA, Brumfield JC, Irene EA, Murray RW (1993) Imaging the incipient electrochemical oxidation of highly oriented pyrolytic graphite. Anal Chem 65:1378–1389. https://doi.org/10.1021/ac00058a014

    Article  CAS  Google Scholar 

  18. Wood EL, Tatke A, Viehmann A, Ashtiani M, Friedman RL, Kopcha M, Fisher AC (2022) Dosage unit uniformity and dissolution testing of extended-release pharmaceutical products marketed in the US. Int J Pharm 625:122119. https://doi.org/10.1016/j.ijpharm.2022.122119

    Article  CAS  Google Scholar 

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Funding

The authors are grateful to FONDECYT (Grant 1160167).

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Authors and Affiliations

  1. Departamento de Química de los Materiales, Facultad de Química y Biología, Universidad de Santiago de Chile, Av.Libertador B.O’Higgins 3363, Santiago, Chile

    Miguel Gulppi, Jorge Pavez Irrazabal, Manuel Ignacio Azócar & Maritza Páez

  2. Institut für Biochemie, Universität Greifswald, Felix-Hausdorff-Str. 4, D-17487, Greifswald, Germany

    Fritz Scholz

Authors
  1. Miguel Gulppi
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  2. Jorge Pavez Irrazabal
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  3. Manuel Ignacio Azócar
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  4. Fritz Scholz
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  5. Maritza Páez
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Correspondence to Miguel Gulppi.

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Gulppi, M., Irrazabal, J.P., Azócar, M.I. et al. The combination of extraction and chronocoulometry of a solid precipitate for teaching a novel approach of electroanalysis. J Solid State Electrochem 28, 677–682 (2024). https://doi.org/10.1007/s10008-023-05764-8

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