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Electroanalytical determination of eugenol in clove oil by voltammetry of immobilized microdroplets

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Abstract

The determination of eugenol employing voltammetry of immobilized microdroplets (VIM) is reported in this work. The construction of the electrode was based on a glassy carbon substrate modified with carbon black nanoparticles and eugenol standard or clove oil sample within a dihexadecyl hydrogen phosphate (DHP) film. The different experimental parameters related to this proposed procedure, i.e., supporting electrolyte composition, pH, ionic strength, and square-wave voltammetry (SWV) parameters (amplitude, frequency, and step potential) were optimized. By doing that, the SWV measurements were performed on the following optimum experimental conditions: 0.2 mol L−1 phosphate buffer solution (pH 2.0), amplitude 90 mV, frequency 30 Hz, and step potential 5 mV. The analytical curve for eugenol was linear in the wide range from 0.03 to 26 μg (r = 0.999) with limits of detection (LOD) and quantification (LOQ) of 0.13 and 0.42 ng, respectively. Three commercial clove oil samples were successfully immobilized and analyzed using the proposed VIM procedure showing eugenol concentrations ranging from 14.0 to 68.3%. Comparing the results obtained by the proposed and comparative procedure (spectrophotometric), relative standard deviations (RSDs) lower than 5.0% were verified, demonstrating the concordance of the results obtained by both methods.

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Acknowledgments

We would like to thank CEME-SUL FURG and CAB English Lessons for the English correction.

Funding

We gratefully acknowledge the financial support of the following Brazilian Funding Agencies CNPq (444150/2014-5) and CAPES.

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Authors and Affiliations

  1. School of Chemical and Food, Federal University of Rio Grande, Av. Itália, km 8, P. O. Box 474, Rio Grande, RS, 96203-900, Brazil

    Juliana Villela Maciel & Daiane Dias

  2. Department of Chemistry, Federal University of São Carlos, Rod. Washington Luís km 235, P. O. Box 676, São Carlos, SP, 13560-970, Brazil

    Tiago Almeida Silva & Orlando Fatibello-Filho

Authors
  1. Juliana Villela Maciel
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  2. Tiago Almeida Silva
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  3. Daiane Dias
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  4. Orlando Fatibello-Filho
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Correspondence to Daiane Dias.

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Maciel, J.V., Silva, T.A., Dias, D. et al. Electroanalytical determination of eugenol in clove oil by voltammetry of immobilized microdroplets. J Solid State Electrochem 22, 2277–2285 (2018). https://doi.org/10.1007/s10008-018-3933-z

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  • DOI: https://doi.org/10.1007/s10008-018-3933-z

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