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Novel potential type electrochemical chiral recognition biosensor for amino acid

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Abstract

Novel potential type electrochemical chiral biosensing system with unique capability of distinguishing and quantitating of tyrosine (Tyr) enantiomers by L-cysteic acid and left-handed chiral carbonaceous nanotubes (L-CCNT) modified glassy carbon electrode (L-Cys/L-CCNT/GCE) was first developed. The effect of sweep cycles of L-Cys and the kinds of L-CCNT on electrochemical chiral biosensing performance of L-Cys/L-CCNT/GCE were investigated. The electrochemical identification and quantitative determination of L- and D-tyrosine in their mixed solution were successfully achieved based on the different oxidation potential signals. The chiral structure of L-CCNT, the aromatic ring of Tyr, and also the intermolecular hydrogen bond between cysteic acid (CyA) and Tyr could possibly produce the difference in the free energy, which reflects as potential difference of L- and D-tyrosine. A good linear relationship between the potential, current, and different concentration ratios of L- and D-Tyr was obtained. Our present work realizes the simultaneous detection of Tyr enantiomers in their mixed solution based on the different potential signals, and it is of far-reaching significance in real electrochemical chiral biosensor study.

Construction of chiral recognition interface and the chiral biosensing mechanism for L-Tyr and D-Tyr

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Acknowledgements

The authors gratefully acknowledge the support from the National Natural Science Foundation of China (No. 21505031), colleges and universities science technology research project of Hebei Province (No. Z2015096) and the Natural Science Foundation of Hebei Province (No. B2016201018).

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Authors and Affiliations

  1. Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, 071002, Baoding, People’s Republic of China

    Yanyang Guo, Runrun Yao, Zimeng Wang, Yufan Zhang, Mengjing Cui, Qiuyue Zhao & Huan Wang

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  1. Yanyang Guo
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  2. Runrun Yao
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  3. Zimeng Wang
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  4. Yufan Zhang
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  5. Mengjing Cui
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  6. Qiuyue Zhao
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  7. Huan Wang
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Correspondence to Yufan Zhang or Huan Wang.

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Guo, Y., Yao, R., Wang, Z. et al. Novel potential type electrochemical chiral recognition biosensor for amino acid. J Solid State Electrochem 22, 41–49 (2018). https://doi.org/10.1007/s10008-017-3719-8

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  • DOI: https://doi.org/10.1007/s10008-017-3719-8

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