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Electrochemical and microgravimetric studies of poly[3,4-ethylenedioxythiophene]-tyrosinase biocomposite material electrodeposited onto gold electrodes by a sinusoidal voltages method

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Abstract

The electrodeposition of poly(3,4-ethylenedioxythiophene)-tyrosinase (PEDOT-Ty) biocomposite material onto gold electrode has been achieved by means of a sinusoidal voltages (SV) method. The SV method consists in the superimposition of a sinusoidal voltage (sin wave) with fixed frequency and amplitude onto a d.c. potential. The influence of electrochemical parameters like frequency and amplitude of SV signal, d.c. potential value, deposition time, on the electrodeposition process has been investigated. The biocomposite material has been prepared as a thin layer onto quartz crystals coated with gold. The frequency change of the quartz crystal during the electrodeposition of the biocomposite material was recorded simultaneously with the measured current response. The morphology of the deposited PEDOT-Ty coatings was investigated by scanning electron microscopy. The electrochemical behavior of the biocomposite material in aqueous solution was studied by cyclic voltammetry and electrochemical impedance spectroscopy. The use of d.c. potential value of 0.6 V revealed the contribution of the SV component to the electrodeposition process. The PEDOT-Ty modified electrode was used as an electrochemical biosensor in the voltammetric determination of dopamine in the presence of hydroquinone. The analytical performances of the prepared biosensors were also investigated.

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Acknowledgments

This work was supported by a grant from the Romanian National Authority for Scientific Research, CNCS–UEFISCDI, Project number PN-II-ID-PCE-2011-3-0271 (298/06.10.2011).

Author information

Authors and Affiliations

  1. Institute of Physical Chemistry “Ilie Murgulescu” of the Romanian Academy, 202 Splaiul Independentei, 060021, Bucharest, Romania

    Cecilia Lete

  2. Department of Inorganic Chemistry, Physical Chemistry and Electrochemistry, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu Street, 011061, Bucharest, Romania

    Daniela Berger & Cristian Matei

  3. Department of Analytical Chemistry and Environmental Engineering, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu Street, 011061, Bucharest, Romania

    Stelian Lupu

Authors
  1. Cecilia Lete
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  2. Daniela Berger
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  3. Cristian Matei
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  4. Stelian Lupu
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Corresponding author

Correspondence to Stelian Lupu.

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We wish to Professor George Inzelt happiness, good health, and success on this occasion of his 70th birthday.

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Lete, C., Berger, D., Matei, C. et al. Electrochemical and microgravimetric studies of poly[3,4-ethylenedioxythiophene]-tyrosinase biocomposite material electrodeposited onto gold electrodes by a sinusoidal voltages method. J Solid State Electrochem 20, 3043–3051 (2016). https://doi.org/10.1007/s10008-016-3270-z

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  • DOI: https://doi.org/10.1007/s10008-016-3270-z

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