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Spectroscopic and electrochemical studies on the interaction between luteolin and DNA

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Abstract

The interactions of luteolin (LUT) with DNA, including double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA), were investigated in detail by ultraviolet–visible spectroscopy (UV–vis), fluorescence spectrometry, and electrochemical methods. Based on the spectroscopic results, we deduced that LUT interacted with DNA mainly via intercalative mode because the maximum ultraviolet–visible absorption of LUT displayed a hyperchromic effect with a red shift in the presence of DNA, and the fluorescence of LUT was quenched by DNA. Based on the voltammetric behaviors of LUT on a reduced graphene oxide (RGO)-modified glassy carbon electrode (RGO/GCE), some electrochemical parameters related to LUT, such as number of electron transferred (n) and the standard rate constant of the electrode reaction (K s), were calculated before and after it interacted with DNA. The binding ratio (m) of LUT with DNA and the equilibrium constant (β) were also calculated. These results could provide useful information for insight into pharmacological mechanism of LUT.

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Acknowledgments

The work was supported by the National Natural Science Foundation of China (No. 21105023) and the Natural Science Foundation Committee of Shandong Province, China (No. BS2013HZ027), and China Postdoctoral Science Foundation Funded Project (No. 2015M572040).

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Authors and Affiliations

  1. Department of Chemistry and Chemical Engineering, Heze University, Heze, Shandong, 274015, China

    Na Zou & Xueliang Wang

  2. School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong, 250010, China

    Xueliang Wang

  3. Department of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, Gansu, 730070, China

    Guifang Li

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  1. Na Zou
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  2. Xueliang Wang
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  3. Guifang Li
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Correspondence to Xueliang Wang.

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Zou, N., Wang, X. & Li, G. Spectroscopic and electrochemical studies on the interaction between luteolin and DNA. J Solid State Electrochem 20, 1775–1782 (2016). https://doi.org/10.1007/s10008-016-3174-y

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  • DOI: https://doi.org/10.1007/s10008-016-3174-y

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