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Direct electrochemistry of glucose oxidase immobilized on porous carbon nanofiber/room temperature ionic liquid/chitosan composite film and its biosensing application

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Abstract

The direct electron transfer of glucose oxidase (GOD) immobilized on a composite matrix based on porous carbon nanofibers (PCNFs), room-temperature ionic liquid (RTIL), and chitosan (CHIT) underlying on a glassy carbon electrode was achieved. The combination of the PCNFs, RTIL, and CHIT provided a suitable microenvironment for GOD to transfer electron directly. In deaerated buffer solutions (pH 7.0), the cyclic voltammetry of the GOD/PCNFs/RTIL/CHIT composite films showed a pair of well-defined redox peaks with the formal potential of −0.45 V (vs. SCE). The synergistic effort of the PCNFs, RTIL, and CHIT also promoted the stability of GOD in the composite film and retained its bioactivity.

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Acknowledgments

The authors appreciate the financial support from the National Natural Science Foundation of China (no. 20875076), the Science Foundation of Northwest University (no. 09NW02), the Education Department of Shaanxi Province, China (no. 2010JK877), and the NWU Doctorate Dissertation of Excellence Funds (no. 08YYB06).

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Authors and Affiliations

  1. Institute of Analytical Science/Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi’an, Shaanxi, 710069, China

    Qinglin Sheng, Ruixiao Liu & Jianbin Zheng

  2. Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, 62102, Taiwan, Republic of China

    Wanghua Lin & Yuanyao Li

Authors
  1. Qinglin Sheng
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  2. Ruixiao Liu
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  3. Jianbin Zheng
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  4. Wanghua Lin
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  5. Yuanyao Li
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Correspondence to Jianbin Zheng.

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Sheng, Q., Liu, R., Zheng, J. et al. Direct electrochemistry of glucose oxidase immobilized on porous carbon nanofiber/room temperature ionic liquid/chitosan composite film and its biosensing application. J Solid State Electrochem 16, 739–745 (2012). https://doi.org/10.1007/s10008-011-1420-x

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  • DOI: https://doi.org/10.1007/s10008-011-1420-x

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