Abstract
Purpose
Augmentation of the maxillary sinus floor with bone grafting is commonly used for successful treatment of edentulous posterior maxilla with dental implants, and it is essential to maintain good bone volume and quality for long-term success of dental implants. The aim of this experimental study was to investigate the local and systemic effects of boric acid on new bone formation after maxillary sinus floor augmentation (MSFA).
Materials and methods
Twenty-four male, New Zealand rabbits were randomly divided into three groups with eight rabbits each, and bilateral MSFA was performed in each animal. An autogenous bone/xenograft mixture was used to augment the maxillary sinuses in each group. Group 1 was determined as control with no additional materials, whereas 3 mg/kg boric acid (BA) was added to the mixture in group 2, and 3 mg/kg boric acid solution added to drinking water daily in group 3.
Results
The animals were sacrificed and also histologic, histomorphometric, and immunnohistochemical analyses were performed at weeks 4 and 8. At week 4, bone regeneration was better in the local BA group than in the control and systemic BA groups (p < 0.05). However, no significant difference was found among the groups in terms of bone regeneration at the end of week 8 (p > 0.05).
Conclusion
Significant higher new bone formation was revealed by BA at early healing especially with local application. BA may be a therapeutic option for improving the bone regeneration.
Similar content being viewed by others
References
Boyne PJ, James RA (1980) Grafting of the maxillary sinus floor with autogenous marrow and bone. J Oral Surg 38:613–616
Gutwald R, Haberstroh J, Stricker A, Ruther E, Otto F, Xavier SP, Oshima T, Marukawa E, Seto I, Enomoto S, Hoogendijk CF, Schmelzeisen R, Sauerbier S (2010) Influence of rhbmp-2 on bone formation and osseointegration in different implant systems after sinus-floor elevation. An in vivo study on sheep. J Craniomaxillofac Surg 38:571–579
Rickert D, Slater JJ, Meijer HJ, Vissink A, Raghoebar GM (2012) Maxillary sinus lift with solely autogenous bone compared to a combination of autogenous bone and growth factors or (solely) bone substitutes. A systematic review. Int J Oral Maxillofac Surg 41:160–167
Raghoebar GM, Brouwer TJ, Reintsema H, Van Oort RP (1993) Augmentation of the maxillary sinus floor with autogenous bone for the placement of endosseous implants: a preliminary report. J Oral Maxillofac Surg 51:1198–1203; discussion 1203-1195
Arrington ED, Smith WJ, Chambers HG, Bucknell AL, Davino NA (1996) Complications of iliac crest bone graft harvesting. Clin Orthop Relat Res 329:300–309
Cricchio G, Lundgren S (2003) Donor site morbidity in two different approaches to anterior iliac crest bone harvesting. Clin Implant Dent Relat Res 5:161–169
Devirian TA, Volpe SL (2003) The physiological effects of dietary boron. Crit Rev Food Sci Nutr 43:219–231
Hunt CD (2012) Dietary boron: progress in establishing essential roles in human physiology. J Trace Elem Med Biol 26:157–160
Gorustovich AA, Steimetz T, Nielsen FH, Guglielmotti MB (2008) A histomorphometric study of alveolar bone modelling and remodelling in mice fed a boron-deficient diet. Arch Oral Biol 53:677–682
Sutherland B, Strong P, King JC (1998) Determining human dietary requirements for boron. Biol Trace Elem Res 66:193–204
Kim BJ, Kwon TK, Baek HS, Hwang DS, Kim CH, Chung IK, Jeong JS, Shin SH (2012) A comparative study of the effectiveness of sinus bone grafting with recombinant human bone morphogenetic protein 2-coated tricalcium phosphate and platelet-rich fibrin-mixed tricalcium phosphate in rabbits. Oral Surg Oral Med Oral Pathol Oral Radiol 113:583–592
Browaeys H, Bouvry P, De Bruyn H (2007) A literature review on biomaterials in sinus augmentation procedures. Clin Implant Dent Relat Res 9:166–177
Khairy NM, Shendy EE, Askar NA, El-Rouby DH (2013) Effect of platelet rich plasma on bone regeneration in maxillary sinus augmentation (randomized clinical trial). Int J Oral Maxillofac Surg 42:249–255
Ohya M, Yamada Y, Ozawa R, Ito K, Takahashi M, Ueda M (2005) Sinus floor elevation applied tissue-engineered bone. Comparative study between mesenchymal stem cells/platelet-rich plasma (prp) and autogenous bone with prp complexes in rabbits. Clin Oral Implants Res 16:622–629
Tonetti MS, Hammerle CH (2008) Advances in bone augmentation to enable dental implant placement: consensus report of the sixth European workshop on periodontology. J Clin Periodontol 35:168–172
Srouji S, Ben-David D, Lotan R, Riminucci M, Livne E, Bianco P (2010) The innate osteogenic potential of the maxillary sinus (Schneiderian) membrane: an ectopic tissue transplant model simulating sinus lifting. Int J Oral Maxillofac Surg 39:793–801
Komurcu E, Ozyalvacli G, Kaymaz B, Golge UH, Goksel F, Cevizci S, Adam G, Ozden R (2015) Effects of local administration of boric acid on posterolateral spinal fusion with autogenous bone grafting in a rodent model. Biol Trace Elem Res 167:77–83
Hunt CD, Herbel JL, Idso JP (1994) Dietary boron modifies the effects of vitamin d3 nutrition on indices of energy substrate utilization and mineral metabolism in the chick. J Bone Miner Res 9:171–182
Armstrong TA, Spears JW, Crenshaw TD, Nielsen FH (2000) Boron supplementation of a semipurified diet for weanling pigs improves feed efficiency and bone strength characteristics and alters plasma lipid metabolites. J Nutr 130:2575–2581
Chapin RE, Ku WW, Kenney MA, McCoy H (1998) The effects of dietary boric acid on bone strength in rats. Biol Trace Elem Res 66:395–399
Chapin RE, Ku WW, Kenney MA, McCoy H, Gladen B, Wine RN, Wilson R, Elwell MR (1997) The effects of dietary boron on bone strength in rats. Fundam Appl Toxicol 35:205–215
Ying X, Cheng S, Wang W, Lin Z, Chen Q, Zhang W, Kou D, Shen Y, Cheng X, Rompis FA, Peng L, Zhu Lu C (2011) Effect of boron on osteogenic differentiation of human bone marrow stromal cells. Biol Trace Elem Res 144:306–315
Uysal T, Ustdal A, Sonmez MF, Ozturk F (2009) Stimulation of bone formation by dietary boron in an orthopedically expanded suture in rabbits. Angle Orthod 79:984–990
Golge UH, Kaymaz B, Arpaci R, Komurcu E, Goksel F, Guven M, Guzel Y, Cevizci S (2015) Effects of boric acid on fracture healing: an experimental study. Biol Trace Elem Res 167:264–271
Aubin JE (2000) Osteogenic cell differentiation. In: Davies JE, editor. Bone engineering. Toronto, Canada: em squared Inc. pp. 19–39
Knabe C, Koch C, Rack A, Stiller M (2008) Effect of beta-tricalcium phosphate particles with varying porosity on osteogenesis after sinus floor augmentation in humans. Biomaterials 29:2249–2258
Sodek J, Cheifitz S (2000) Molecular regulation of osteogenesis. In: Davies JE, editor. Bone engineering. Toronto, Canada: em squared Inc. pp. 31–43
Street J, Bao M, deGuzman L, Bunting S, Peale FV Jr, Ferrara N, Steinmetz H, Hoeffel J, Cleland JL, Daugherty A, van Bruggen N, Redmond HP, Carano RA, Filvaroff EH (2002) Vascular endothelial growth factor stimulates bone repair by promoting angiogenesis and bone turnover. Proc Natl Acad Sci U S A 99:9656–9661
Street J, Winter D, Wang JH, Wakai A, McGuinness A, Redmond HP (2000) Is human fracture hematoma inherently angiogenic? Clin Orthop Relat Res 378:224–237
Hall PA, Levison DA, Woods AL, Yu CC, Kellock DB, Watkins JA, Barnes DM, Gillett CE, Camplejohn R, Dover R et al (1990) Proliferating cell nuclear antigen (pcna) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 162:285–294
Funding
This study was financially supported by The Scientific and Technological Research Council of Turkey—Health Sciences Research Group (TUBITAK-SBAG; project number 113S258).
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of interest
The authors declare that they have no conflicts of interest.
Ethical approval
All applicable international, national, and institutional guidelines for the care and use of animals were followed. The protocol for this study was approved by Erciyes University Medical School Animal Care and Use Committee.
Informed consent
This study does not contain any experiment with human participants.
Rights and permissions
About this article
Cite this article
Ulu, M., Kütük, N., Cıcık, M.F. et al. Effects of boric acid on bone formation after maxillary sinus floor augmentation in rabbits. Oral Maxillofac Surg 22, 443–450 (2018). https://doi.org/10.1007/s10006-018-0729-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10006-018-0729-3