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Exploring the differences and similarities between urea and thermally driven denaturation of bovine serum albumin: intermolecular forces and solvation preferences

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Abstract

The interactions of bovine serum albumin (BSA) with urea/water were investigated by computer simulation. It was revealed that the BSA-hydrophobic residues in urea solutions favored contact with urea more than with water. Energy decomposition analysis showed that van der Waals energy was the dominant driving force behind urea affinity for hydrophobic residues, whereas coulombic attraction was largely responsible for water affinity for these residues. Meanwhile, urea–BSA hydrogen bond energies were found to be weaker than water–BSA hydrogen bond energies. The greater strength of water–BSA hydrogen bonds than urea–BSA hydrogen bonds, and the opposing preferential interaction between the BSA and urea suggest that disruption of hydrophobic interaction predominates urea–protein denaturation. In pure water, hydrophobic residues showed aggregation tendencies at 323 K, suggesting an increase in hydrophobicity, while at 353 K the residues were partly denatured due to loss of hydrogen bonds; thus, disruption of hydrophobic interactions appeared to contribute less to thermal denaturation.

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Acknowledgments

This research was supported by the Basic Science Research Program (2015R1D1A1A09057413) through the National Research Foundation of Korea (NRF) and BK21 PLUS Center for Advanced Chemical Technology (21A20131800002).

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  1. School of Chemical and Biomolecular Engineering, Pusan National University, Busan, 609-735, Republic of Korea

    Osita Sunday Nnyigide, Sun-Gu Lee & Kyu Hyun

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  1. Osita Sunday Nnyigide
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  2. Sun-Gu Lee
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  3. Kyu Hyun
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Correspondence to Kyu Hyun.

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Nnyigide, O.S., Lee, SG. & Hyun, K. Exploring the differences and similarities between urea and thermally driven denaturation of bovine serum albumin: intermolecular forces and solvation preferences. J Mol Model 24, 75 (2018). https://doi.org/10.1007/s00894-018-3622-y

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