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Fungal diversity in a sediment core from climate change impacted Boeckella Lake, Hope Bay, north-eastern Antarctic Peninsula assessed using metabarcoding

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Abstract

We studied the fungal DNA present in a lake sediment core obtained from Trinity Peninsula, Hope Bay, north-eastern Antarctic Peninsula, using metabarcoding through high-throughput sequencing (HTS). Sequences obtained were assigned to 146 amplicon sequence variants (ASVs) primarily representing unknown fungi, followed by the phyla Ascomycota, Rozellomycota, Basidiomycota, Chytridiomycota and Mortierellomycota. The most abundant taxa were assigned to Fungal sp., Pseudeurotium hygrophilum, Rozellomycota sp. 1, Pseudeurotiaceae sp. 1 and Chytridiomycota sp. 1. The majority of the DNA reads, representing 40 ASVs, could only be assigned at higher taxonomic levels and may represent taxa not currently included in the sequence databases consulted and/or be previously undescribed fungi. Different sections of the core were characterized by high sequence diversity, richness and moderate ecological dominance indices. The assigned diversity was dominated by cosmopolitan cold-adapted fungi, including known saprotrophic, plant and animal pathogenic and symbiotic taxa. Despite the overall dominance of Ascomycota and Basidiomycota and psychrophilic Mortierellomycota, members of the cryptic phyla Rozellomycota and Chytridiomycota were also detected in abundance. As Boeckella Lake may cease to exist in approaching decades due the effects of local climatic changes, it also an important location for the study of the impacts of these changes on Antarctic microbial diversity.

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Data availability

All raw sequences have been deposited in the NCBI database under the codes SAMN24389534, SAMN24389535 and SAMN24389536.

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Acknowledgements

This study received financial support from CNPq, CAPES, FNDCT, FAPEMIG, INCT Criosfera, PROANTAR. P. Convey is supported by NERC core funding to the British Antarctic Survey’s ‘Biodiversity, Evolution and Adaptation’ Team. We thank the Instituto Antártico Argentino for logistical and financial support for the field campaigns in Antarctica during summers 2018 and 2019, Marie Bulinova and Patricia Ader for their help in the field.

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Authors and Affiliations

  1. Laboratório de Microbiologia Polar e Conexões Tropicais, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, PO Box 486, Belo Horizonte, MG, 31270-901, Brazil

    Luiz Henrique Rosa, Mayara Baptistucci Ogaki & Carlos Augusto Rosa

  2. Instituto Antártico Argentino, Buenos Aires, Argentina

    Juan Manuel Lirio & Silvia H. Coria

  3. Instituto de Geociências, Universidade Federal Fluminense, Niterói, RJ, Brazil

    Rosemary Vieira

  4. Departamento de Biologia Celular, Universidade de Brasília, Brasília, Brazil

    Otávio Henrique Bezerra Pinto

  5. Departamento de Botânica, Universidade de Brasília, Brasília, Brazil

    Micheline Carvalho-Silva & Paulo Eduardo Aguiar Saraiva Câmara

  6. British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge, CB3 0ET, UK

    Peter Convey

  7. Department of Zoology, University of Johannesburg, PO Box 524, Auckland Park, 2006, South Africa

    Peter Convey

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  1. Luiz Henrique Rosa
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Correspondence to Luiz Henrique Rosa.

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The collections and studies performed in Antarctic Peninsula were authorized by the Secretariat of the Antarctic Treaty and by PROANTAR.

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Rosa, L.H., Ogaki, M.B., Lirio, J.M. et al. Fungal diversity in a sediment core from climate change impacted Boeckella Lake, Hope Bay, north-eastern Antarctic Peninsula assessed using metabarcoding. Extremophiles 26, 16 (2022). https://doi.org/10.1007/s00792-022-01264-1

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