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Description of Halegenticoccus soli gen. nov., sp. nov., a halophilic archaeon isolated from a soil sample of Ebi lake

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Abstract

Two extreme halophilic archaeal strains, SYSUA9-0T and SYSUA9-1, were isolated from Ebi lake of Xinjiang, China. The colonies were Gram-negative, coccoid, and non-motile. Strains were aerobic and grew at 25–50 °C (optimum at 37 °C), in the presence of 10–35% (w/v) NaCl (optimum at 20–22%), and pH 6.0–8.0 (optimum at 7.0). The 16S rRNA gene sequence result revealed that the two strains were closely related to Haloprofundus marisrubri SB9T (92.7% similarity). The DNA–DNA hybridization value (97% ± 1%) suggested that SYSUA9-0T and SYSUA9-1 were similar; however, their sequence similarities with other archaeal members suggested that they were novel candidates. The genomic G + C content of SYSUA9-0T was 66.9%. The average nucleotide identity value between SYSU A9-0T and Haloprofundus marisrubri SB9T was 69.1%, which was far below the cutoff value (95–96%) proposed to define the species boundary. The polar lipids were phosphatidylglycerol (PG), phosphatidylglycerolphosphate methylester (PGP-Me), sulfated mannosyl glucosyl diether, mannosyl glucosyldiether, and four unidentified glycolipids. Phenotypic, chemotaxonomic and comparative genome analysis suggested that SYSU A9-0T and SYSU A9-1 represent a novel species of a new genus within the family Haloferacaceae, for which the name Halegenticoccus soli gen. nov., sp. nov., is proposed. The type strain is SYAUA9-0T (= KCTC4241T = CGMCC 1.15765T).

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Acknowledgements

The authors acknowledge Prof. Dr. Yu-Guang Zhou (CGMCC, China) for providing the reference type strains. This research was supported by National Science and Technology Foundation Project (No. 2017FY100300), National Natural Science Foundation of China (Nos. 91751206, 31800001), China Postdoctoral Science Foundation funded project (2015M580748), China Postdoctoral Special Foundation funded project (2016T90811), Natural Science Foundation of Guangdong Province (2017A030310206) and China Biodiversity Observation Networks (Sino BON). This work was also funded by the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University, through the Research Groups Program Grant no. (RGP-1438-0004).

The 16S rRNA gene sequences of strains SYSU A9-0T and SYSU A9-1 have been deposited in GenBank/EMBL/DDBJ under the accession numbers KX752787and KX752786. The rpoB’ gene sequences of SYSU A9-0T and SYSU A9-1 has been deposited in GenBank/EMBL/DDBJ under the accession numbers KX752788 and KX752789, respectively. The whole genome shotgun project of strain SYSU A9-0T has been deposited at DDBJ/EMBL/GenBank under the accession PEND00000000.

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  1. Bing-Bing Liu and Manik Prabhu Narsing Rao contributed equally to this work.

Authors and Affiliations

  1. Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology, College of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang, 473004, People’s Republic of China

    Bing-Bing Liu

  2. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Manik Prabhu Narsing Rao, Xiao-Qing Yin, Xin Li, Nimaichand Salam, Yao Zhang & Wen-Jun Li

  3. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi, 830011, China

    Wen-Jun Li

  4. Biology Department, Faculty of Science, Princess Nourah Bint Abdulrahman University, Riyadh, 11564, Kingdom of Saudi Arabia

    Dalal Hussien M. Alkhalifah

  5. Bioproducts Research Chair, Zoology Department, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

    Wael N. Hozzein

  6. Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt

    Wael N. Hozzein

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  1. Bing-Bing Liu
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Liu, BB., Narsing Rao, M.P., Yin, XQ. et al. Description of Halegenticoccus soli gen. nov., sp. nov., a halophilic archaeon isolated from a soil sample of Ebi lake. Extremophiles 23, 521–528 (2019). https://doi.org/10.1007/s00792-019-01104-9

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