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Tepidibacillus fermentans gen. nov., sp. nov.: a moderately thermophilic anaerobic and microaerophilic bacterium from an underground gas storage

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Abstract

A novel moderately thermophilic bacterium, strain STGHT, was isolated from Severo-Stavropolskoye underground gas storage (Russia). Cells of strain STGHT were spore-forming motile straight rods 0.3 μm in diameter and 2.0–4.0 μm in length having a Gram-positive cell wall structure. The temperature range for growth was 36–65 °C, with an optimum at 50–52 °C. The pH range for growth was 5.5–8.0, with an optimum at pH 7.0–7.5. Growth of strain STGHT was observed at NaCl concentrations ranging from 0 to 4.0 % (w/v) with an optimum at 1.0 % (w/v). Strain STGHT grew anaerobically by reduction of nitrate, thiosulfate, S0 and AQDS using a number of complex proteinaceous compounds, organic acids and carbohydrates as electron donors. Nitrate was reduced to nitrite; thiosulfate and sulfur were reduced to sulfide. It also was able to ferment pyruvate, glucose, fructose, and maltose. The strain STGHT did not grow under aerobic conditions during incubation with atmospheric concentration of oxygen but was able to microaerobic growth (up to 10 % of oxygen in gas phase). The G+C content of DNA of strain STGHT was 34.8 mol%. 16S rRNA gene sequence analysis revealed that the isolated organism belongs to the class Bacilli. We propose to assign strain STGHT to a new species of a novel genus Tepidibacillus fermentans gen. nov., sp.nov. The type strain is STGHT (=DSM 23802T, =VKM B-2671T).

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Acknowledgments

This work was supported by Programs “Molecular and cell biology” and “The origin and evolution of the biosphere” of the Russian Academy of Sciences and by project 14.B37.21.1489 and contracts № 11.519.11.2029 and 14.512.11.0070 from the Ministry of Education and Science of Russia. We are grateful to Drs. I. Borzenkov and A. Tarasov for providing samples and for consultations.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya 7/2, 117312, Moscow, Russia

    G. B. Slobodkina, N. A. Kostrikina, E. A. Bonch-Osmolovskaya & A. I. Slobodkin

  2. Bioengineering Center, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya 7/1, 117312, Moscow, Russia

    A. N. Panteleeva

  3. Gubkin Russian State University of Oil and Gas, Leninskiy Prospect 65, 117485, Moscow, Russia

    D. S. Kopitsyn

Authors
  1. G. B. Slobodkina
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  2. A. N. Panteleeva
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  3. N. A. Kostrikina
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  4. D. S. Kopitsyn
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  5. E. A. Bonch-Osmolovskaya
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  6. A. I. Slobodkin
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Corresponding author

Correspondence to G. B. Slobodkina.

Additional information

Communicated by M. da Costa.

The GenBank/EMBL accession number of the 16S rRNA gene sequence of strain STGHT is KC242245.

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Slobodkina, G.B., Panteleeva, A.N., Kostrikina, N.A. et al. Tepidibacillus fermentans gen. nov., sp. nov.: a moderately thermophilic anaerobic and microaerophilic bacterium from an underground gas storage. Extremophiles 17, 833–839 (2013). https://doi.org/10.1007/s00792-013-0564-2

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  • DOI: https://doi.org/10.1007/s00792-013-0564-2

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