Abstract
A new type of thermophilic cyanobacterial mat, rich in elemental sulfur and containing large numbers of sulfur-reducing bacteria able to utilize different growth substrates at 55° C, was found in the Uzon caldere (Kamchatka). One of the largest groups among these organisms were acetate-oxidizing sulfur-reducing bacteria, numbering 106 cells · cm−3 of mat. The pure culture of a sulfur-reducing eubacterium growing on acetate was isolated. Cells of the new isolate are Gram-negative short rods, often in pairs, motile, with a single polar flagellum. The optimal temperature for growth is 52 to 57° C, with no growth observed at 42 or 73° C. The pH optimum is 6.8 to 7.0. The new isolate is demonstrated to be a true dissimilatory sulfur reducer: it is an obligate anaerobe, it is unable to ferment organic substrates and it can use no electron acceptors other than elemental sulfur. Acetate is the only energy and carbon source, and H2S and CO2 are growth products. No cytochromes were detected. The G+C content of DNA is rather low, only 31.4 mol%. Thus, morphological and physiological features of the new isolate are quite close to those of Desulfuromonas. But on the grounds of a significant difference in the G+C content of DNA, the absence of cytochromes and because of its thermophilic nature, a new genus Desulfurella is proposed with the type species Desulfurella acetivorans.
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References
Bonch-Osmolovskaya EA (1986) Reduction of elemental sulfur in algal-bacterial communities of the Uzon caldere. In: Imshenetski AA (ed) Biology of thermophilic microorganisms. Nauka, Moscow, pp 112–113 (In Russian)
Bonch-Osmolovskaya EA, Gorlenko VM, Karpov GA, Starynin DA (1987) Anaerobic destruction of organic matter in cyanobacterial mats of the Thermophilny spring. Mikrobiologia 56:1022–1028 (in Russian)
Hungate R (1969) A roll-tube method for cultivation of strict anaerobes. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 3B. Academic Press, New York, p 118
Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from microorganisms. J Mol Biol 5:109–118
Nozhevnikova AN, Yagodina TG (1982) A thermophilic acetate methane-producing bacterium. Mikrobiologia 51:642–649 (in Russian)
Pfennig N (1965) Anreicherungskulturen für rote und grüne Schwefelbakterien. Zbl Bakt I Abt Orig [Suppl] I S 179–189
Pfennig N, Lippert KD (1966) Über das Vitamin B12-Bedürfnis phototropher Schwefelbakterien. Arch Mikrobiol 55:245–246
Pfennig N, Biebl H (1976) Desulfuromonas acetoxidans gen. nov and sp. nov., a new anaerobic sulfur-reducing acetate-oxidizing bacterium. Arch Microbiol 110:3–12
Pfennig N, Biebl H (1981) The dissimilatory sulfur-reducing bacteria. In: Starr MP, Stolp H, Trüper H, Balows A, Schlegel H (eds) The prokaryotes. Springer, Berlin Heidelberg New York pp 941–947
Probst J, Bruschi M, Pfennig N, LeGall J (1977) Cytochrome c-551.5 (c7) from Desulfuromonas acetoxidans. Biochim Biophys Acta 460:58–64
Stetter KO, Zillig W (1985) Thermoplasma and the thermophilic sulfur-dependent archaebacteria. In: Woese CR, Wolfe RS (eds) The bacteria. Academic Press, N.Y., L. 8:85–167
Trüper HG, Schlegel HG (1964) Sulfur metabolism in Thiorhodaceae. I. Quantitative measurements on growing cells of Chromatium okenii. Antonie van Leeuvenhoek 30:225–228
Ward DM, Beck E, Revsbech NP, Sandbeck KA, Winfrey MR (1984) Decomposition of hot spring microbial mat. In: Cohen J, Castenholz R, Halvorson HO (eds) Microbial mats: stromatolites. Alan R. Liss, New York, pp 191–214
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Bonch-Osmolovskaya, E.A., Sokolova, T.G., Kostrikina, N.A. et al. Desulfurella acetivorans gen. nov. and sp. nov. —a new thermophilic sulfur-reducing eubacterium. Arch. Microbiol. 153, 151–155 (1990). https://doi.org/10.1007/BF00247813
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DOI: https://doi.org/10.1007/BF00247813