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Characterization of bacterial strains capable of desulphurisation in soil and sediment samples from Antarctica

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Abstract

The presence of sulphur in fossil fuels and the natural environment justifies the study of sulphur-utilising bacterial species and genes involved in the biodesulphurisation process. Technology has been developed based on the natural ability of microorganisms to remove sulphur from polycyclic aromatic hydrocarbon chains. This biotechnology aims to minimise the emission of sulphur oxides into the atmosphere during combustion and prevent the formation of acid rain. In this study, the isolation and characterization of desulphurising microorganisms in rhizosphere and bulk soil samples from Antarctica that were either contaminated with oil or uncontaminated was described. The growth of selected isolates and their capacity to utilise sulphur based on the formation of the terminal product of desulphurisation via the 4S pathway, 2-hydroxybiphenyl, was analysed. DNA was extracted from the isolates and BOX-PCR and DNA sequencing were performed to obtain a genomic diversity profile of cultivable desulphurising bacterial species. Fifty isolates were obtained showing the ability of utilising dibenzothiophene as a substrate and sulphur source for maintenance and growth when plated on selective media. However, only seven genetically diverse isolates tested positive for sulphur removal using the Gibbs assay. DNA sequencing revealed that these isolates were related to the genera Acinetobacter and Pseudomonas.

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Acknowledgments

We would like to thank Dr. Alexandre Rosado for providing samples from Antarctica and for relevant information regarding the material, Dr. Raquel Peixoto and Dr. Juliano Cury for the DNA sequencing, and Dr. Silvana Queiroz for the DNA purification. This study received financial and logistic support from the Brazilian Antarctic Program, PROANTAR.

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Authors and Affiliations

  1. Graduate Program in Environmental Engineering, Institute of Biological and Exact Sciences, Federal University of Ouro Preto, Ouro Preto, MG, Brazil

    Douglas Boniek

  2. Graduate Program in Agricultural Microbiology, Microbiology Department, Federal University of Viçosa, Viçosa, MG, Brazil

    Victor Satler Pylro

  3. Biodiversity and Microbial Ecology Laboratory, Biological Sciences Department, Institute of Biological and Exact Sciences, Federal University of Ouro Preto, Ouro Preto, MG, 35400-000, Brazil

    Débora Figueiredo & Gabriela Frois Duarte

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  1. Douglas Boniek
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  2. Débora Figueiredo
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  3. Victor Satler Pylro
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  4. Gabriela Frois Duarte
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Corresponding author

Correspondence to Gabriela Frois Duarte.

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Communicated by T. Matsunaga.

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Boniek, D., Figueiredo, D., Pylro, V.S. et al. Characterization of bacterial strains capable of desulphurisation in soil and sediment samples from Antarctica. Extremophiles 14, 475–481 (2010). https://doi.org/10.1007/s00792-010-0326-3

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  • DOI: https://doi.org/10.1007/s00792-010-0326-3

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