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Final irrigation protocols affect radicular dentin DMP1-CT expression, microhardness, and biochemical composition

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Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Objective

To evaluate the effect of several final irrigation protocols on radicular dentin microhardness, biochemical composition, and DMP1-CT expression.

Materials and methods

A total of 140 single-rooted human teeth were prepared with WaveOne Gold files and randomly distributed into 7 groups (n = 20) according to the final irrigation protocol: distilled water (DW); sodium hypochlorite-EDTA (NaOCl-EDTA); EDTA (EDTA); EDTA-NaOCl (EDTA-NaOCl); EDTA-chlorhexidine (EDTA-CHX); passive ultrasonic irrigation (PUI:NaOCl-EDTA); and PUI:NaOCl-EDTA-NaOCl. Dentin microhardness (n = 10) was evaluated in the root canal lumen using Vickers hardness tester. Immunohistochemical analysis (n = 5) was used to evaluate DMP1-CT expression. Dentin ultrastructure and biochemical composition were evaluated by using Raman and energy dispersive X‐ray analysis (EDAX) (n = 5) with a scanning electron microscope (SEM). Analysis of variance (ANOVA) and Tukey test were performed (p˂0.05).

Results

Raman spectra of the organic content and DMP1-CT expression were lower at the lumen canal in EDTA-NaOCl, PUI:NaOCl-EDTA, and PUI:NaOCl-EDTA-NaOCl when compared to control (p < 0.05). EDAX showed reduced values for calcium and phosphorus in EDTA-NaOCl, PUI:NaOCl-EDTA, and PUI:NaOCl-EDTA-NaOCl. SEM microphotography’s showed completely cleaned dentin, permeable tubules, and dentin erosion, mainly when PUI was used. NaOCl-EDTA presented significantly higher microhardness values than PUI:NaOCl-EDTA-NaOCl (p < 0.05). PUI:NaOCl-EDTA-NaOCl exhibited the lowest Vickers hardness values of all groups.

Conclusion

The final irrigation protocols that used a final rinse with NaOCl and PUI showed a detrimental effect on radicular dentin DMP1-CT expression, biochemical composition, and microhardness.

Clinical relevance

The adequate irrigation protocol could be advantageous to preserve the radicular dentin ultrastructure, promote adequate adhesion, and sustain favorable conditions for biomineralization and regeneration.

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References

  1. Wagner MH, da Rosa RA, de Figueiredo JAP et al (2017) Final irrigation protocols may affect intraradicular dentin ultrastructure. Clin Oral Investig 21:2173–2182. https://doi.org/10.1007/s00784-016-2006-x

    Article  PubMed  Google Scholar 

  2. Carvalho MPM, Morari VHC, Susin AH et al (2017) Endodontic irrigation protocols: effects on bonding of adhesive systems to coronal enamel and dentin. J Esthet Restor Dent 29:222–228. https://doi.org/10.1111/jerd.12289

    Article  PubMed  Google Scholar 

  3. Oyarzún A, Cordero AM, Whittle M (2002) Immunohistochemical evaluation of the effects of sodium hypochlorite on dentin collagen and glycosaminoglycans. J Endod 28:152–156. https://doi.org/10.1097/00004770-200203000-00002

    Article  PubMed  Google Scholar 

  4. Galler KM, Buchalla W, Hiller KA et al (2015) Influence of root canal disinfectants on growth factor release from dentin. J Endod 41:363–368. https://doi.org/10.1016/j.joen.2014.11.021

    Article  PubMed  Google Scholar 

  5. Dotto L, Sarkis Onofre R, Bacchi A, Rocha Pereira GK (2020) Effect of root canal irrigants on the mechanical properties of endodontically treated teeth: a scoping review. J Endod 46:596-604.e3. https://doi.org/10.1016/j.joen.2020.01.017

    Article  PubMed  Google Scholar 

  6. Cruz-Filho AM, Sousa-Neto MD, Savioli RN et al (2011) Effect of chelating solutions on the microhardness of root canal lumen dentin. J Endod 37:358–362. https://doi.org/10.1016/j.joen.2010.12.001

    Article  PubMed  Google Scholar 

  7. Elbahary S, Haj-yahya S, Khawalid M et al (2020) Effects of different irrigation protocols on dentin surfaces as revealed through quantitative 3D surface texture analysis. Sci Rep 10:1–10. https://doi.org/10.1038/s41598-020-79003-9

    Article  Google Scholar 

  8. Cardoso LR, Baldasso FER, Delai D et al (2019) Effect of EDTA, sodium, and calcium hypochlorite on the inorganic component of root canal dentin: a SEM analysis. Microsc Res Tech 82:128–133. https://doi.org/10.1002/jemt.23151

    Article  PubMed  Google Scholar 

  9. Wang Z, Maezono H, Shen Y, Haapasalo M (2016) Evaluation of root canal dentin erosion after different irrigation methods using energy-dispersive X-ray spectroscopy. J Endod 42:1834–1839. https://doi.org/10.1016/j.joen.2016.07.024

    Article  PubMed  Google Scholar 

  10. Baldasso FER, Roleto L, Silva VDD et al (2017) Effect of final irrigation protocols on microhardness reduction and erosion of root canal dentin. Braz Oral Res 31:e40. https://doi.org/10.1590/1807-3107BOR-2017.vol31.0040

    Article  PubMed  Google Scholar 

  11. Galler KM, Widbiller M, Buchalla W, et al (2015) EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cells. Int Endod J 1–10https://doi.org/10.1111/iej.12492

  12. Retana-Lobo C, Guerreiro-Tanomaru JM, Tanomaru-Filho M et al (2020) Non-collagenous dentin protein binding sites control mineral formation during the biomineralisation process in radicular dentin. Materials (Basel) 13:1053. https://doi.org/10.3390/ma13051053

    Article  Google Scholar 

  13. Oya K, Ishida K, Nishida T et al (2017) Immunohistochemical analysis of dentin matrix protein 1 (Dmp1) phosphorylation by Fam20C in bone: implications for the induction of biomineralization. Histochem Cell Biol 147:341–351. https://doi.org/10.1007/s00418-016-1490-z

    Article  PubMed  Google Scholar 

  14. Qin C, D’Souza R, Feng JQ (2007) Dentin matrix protein 1 (DMP1): new and important roles for biomineralization and phosphate homeostasis. J Dent Res 86:1134–1141. https://doi.org/10.1177/154405910708601202

    Article  PubMed  Google Scholar 

  15. Orsini G, Ruggeri A, Mazzoni A et al (2008) Immunohistochemical localization of dentin matrix protein 1 in human dentin. Eur J Histochem 52:215–220. https://doi.org/10.4081/1219

    Article  PubMed  Google Scholar 

  16. (2016) AAE clinical considerations for a regenerative procedure revised 6/8/2016. https://doi.org/10.2334/josnusd.57.213

  17. Khademi A, Yazdizadeh M, Feizianfard M (2006) Determination of the minimum instrumentation size for penetration of irrigants to the apical third of root canal systems. J Endod 32:417–420. https://doi.org/10.1016/j.joen.2005.11.008

    Article  PubMed  Google Scholar 

  18. Zhang K, Kim YK, Cadenaro M et al (2010) Effects of different exposure times and concentrations of sodium hypochlorite/ethylenediaminetetraacetic acid on the structural integrity of mineralized dentin. J Endod 36:105–109. https://doi.org/10.1016/j.joen.2009.10.020

    Article  PubMed  Google Scholar 

  19. Chan R, Versiani MA, Friedman S et al (2019) Efficacy of 3 supplementary irrigation protocols in the removal of hard tissue debris from the mesial root canal system of mandibular molars. J Endod 45:923–929. https://doi.org/10.1016/j.joen.2019.03.013

    Article  PubMed  Google Scholar 

  20. Ozasir T, Eren B, Gulsahi K, Ungor M (2021) The effect of different final irrigation regimens on the dentinal tubule penetration of three different root canal sealers: a confocal laser scanning microscopy study in vitro. Scanning 2021:1–9. https://doi.org/10.1155/2021/8726388

    Article  Google Scholar 

  21. Doǧan H, Çalt S (2001) Effects of chelating agents and sodium hypochlorite on mineral content of root dentin. J Endod 27:578–580. https://doi.org/10.1097/00004770-200109000-00006

    Article  PubMed  Google Scholar 

  22. Sayin TC, Serper A, Cehreli ZC, Kalayci S (2007) Calcium loss from root canal dentin following EDTA, EGTA, EDTAC, and tetracycline-HCl treatment with or without subsequent NaOCl irrigation. J Endod 33:581–584. https://doi.org/10.1016/j.joen.2006.12.010

    Article  PubMed  Google Scholar 

  23. Aksel H, Albanyan H, Bosaid F, Azim AA (2020) Dentin conditioning protocol for regenerative endodontic procedures. J Endod 46:1099–1104. https://doi.org/10.1016/j.joen.2020.05.010

    Article  PubMed  Google Scholar 

  24. Hu X, Peng Y, Sum CP, Ling J (2010) Effects of concentrations and exposure times of sodium hypochlorite on dentin deproteination: attenuated total reflection fourier transform infrared spectroscopy study. J Endod 36:2008–2011. https://doi.org/10.1016/j.joen.2010.08.035

    Article  PubMed  Google Scholar 

  25. Dumitriu D, Dobre T (2015) Effects of temperature and hypochlorite concentration on the rate of collagen dissolution. J Endod 41:903–906. https://doi.org/10.1016/j.joen.2014.12.020

    Article  PubMed  Google Scholar 

  26. Retana-Lobo C, Guerreiro-Tanomaru JM, Tanomaru-Filho M, Mendes de Souza BD, Reyes-Carmona J (2021) Sodium hypochlorite and chlorhexidine downregulates MMPs expression on radicular dentin. Med Princ Pr 1–13. https://doi.org/10.1159/000517887

  27. Dunavant TR, Regan JD, Glickman GN et al (2006) Comparative evaluation of endodontic irrigants against Enterococcus faecalis biofilms. J Endod 32:527–531. https://doi.org/10.1016/j.joen.2005.09.001

    Article  PubMed  Google Scholar 

  28. Estrela C, Estrela CRA, Barbin EL et al (2002) Mechanism of action of sodium hypochlorite. Braz Dent J 13:113–117. https://doi.org/10.1590/S0103-64402002000200007

    Article  PubMed  Google Scholar 

  29. Teixeira CS, Felippe MCS, Felippe WT (2005) The effect of application time of EDTA and NaOCl on intracanal smear layer removal: an SEM analysis. Int Endod J 38:285–290. https://doi.org/10.1111/j.1365-2591.2005.00930.x

    Article  PubMed  Google Scholar 

  30. Galler KM, Widbiller M, Buchalla W et al (2016) EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cells. Int Endod J 49:581–590. https://doi.org/10.1111/iej.12492

    Article  PubMed  Google Scholar 

  31. Qian W, Shen Y, Haapasalo M (2011) Quantitative analysis of the effect of irrigant solution sequences on dentin erosion. J Endod 37:1437–1441. https://doi.org/10.1016/j.joen.2011.06.005

    Article  PubMed  Google Scholar 

  32. Tanomaru-Filho M, Miano LM, Chávez-Andrade GM et al (2015) Cleaning of root canal system by different irrigation methods. J Contemp Dent Pract 16:859–863. https://doi.org/10.5005/JP-JOURNALS-10024-1771

    Article  PubMed  Google Scholar 

  33. Siqueira JF Jr, Rôças IN, Favieri A, Lima KC (2000) Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite. J Endod 26(6):331–4. https://doi.org/10.1097/00004770-200006000-00006

    Article  PubMed  Google Scholar 

  34. Shrestha A, Friedman S, Kishen A (2011) Photodynamically crosslinked and chitosan-incorporated dentin collagen. J Dent Res 90:1346–1351

    Article  Google Scholar 

  35. Shrestha A, Hamblin MR, Kishen A (2014) Photoactivated rose bengal functionalized chitosan nanoparticles produce antibacterial/biofilm activity and stabilize dentin-collagen. Nanomedicine 10(3):491–501

    Article  Google Scholar 

  36. Rath PP, Yiu CKY, Matinlinna JP, Kishen A, Neelakantan P, (2020) The effect of root canal irrigants on dentin: a focused review. Restor Dent Endod 30;45(3):e39.  https://doi.org/10.5395/rde.2020.45.e39.

  37. Elnawam H, Abdelmougod M, Mobarak A, Hussein M, Aboualmakarem H, Girgis M, El Backly R (2022) Regenerative endodontics and minimally invasive dentistry: intertwining paths crossing over into clinical translation. Front Bioeng Biotechnol 8(10):837639. https://doi.org/10.3389/fbioe.2022.837639

    Article  Google Scholar 

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Funding

This work was supported by LICIFO and Vicerrectoria de Investigación, Universidad de Costa Rica.

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Authors and Affiliations

  1. Department of Endodontics, Faculty of Dentistry, LICIFO, University of Costa Rica, SJO, Sabanilla, San José, 11502, Costa Rica

    Cristina Retana-Lobo, Tatiana Ramírez-Mora & Jessie Reyes-Carmona

  2. Dental Materials Research Laboratory, School of Dentistry, University of Costa Rica, SJO, Sabanilla, San José, 11502, Costa Rica

    Fabian Murillo-Gómez

  3. Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, SP, Brazil

    Juliane Maria Guerreiro-Tanomaru & Mario Tanomaru-Filho

Authors
  1. Cristina Retana-Lobo
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  2. Tatiana Ramírez-Mora
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  3. Fabian Murillo-Gómez
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  5. Mario Tanomaru-Filho
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  6. Jessie Reyes-Carmona
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Correspondence to Jessie Reyes-Carmona.

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The research protocol was approved by the Ethics Committee of the Universidad de Costa Rica (VR‐467‐2018).

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Retana-Lobo, C., Ramírez-Mora, T., Murillo-Gómez, F. et al. Final irrigation protocols affect radicular dentin DMP1-CT expression, microhardness, and biochemical composition. Clin Oral Invest 26, 5491–5501 (2022). https://doi.org/10.1007/s00784-022-04516-8

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  • DOI: https://doi.org/10.1007/s00784-022-04516-8

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