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Final irrigation protocols may affect intraradicular dentin ultrastructure

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Abstract

Objectives

The aim of this study was to evaluate the effect of different irrigation protocols on the root dentin structure using scanning electron microscopy (SEM) and transmission electron microscopy (TEM).

Materials and methods

Thirty-nine lower bovine incisors were hemisected longitudinally and randomly divided into 13 groups (n = 3). After the root halves were reassembled, it was applied a specific irrigation protocol for each group, as following: G1, distilled water (control); G2, 0.9% saline; G3, saline + 17% EDTA; G4, saline + PUI; G5, saline + PUI + EDTA; G6 to G9 received the same protocol as above replacing 0.9% saline by 2.5% NaOCl; and G10 to G13 by 2% CHX. One-half of each sample was prepared and evaluated using SEM and the other one by TEM observations.

Results

TEM descriptive analysis showed modifications in dentin organic ultrastructure, characterized by the thinning of dentin collagen fibrils, caused by NaOCl, enhanced by EDTA and/or PUI. SEM analysis showed that NaOCl with PUI caused significantly larger erosion of the peritubular dentin than in all the other groups (P < 0.05), followed by NaOCl + EDTA and NaOCl + EDTA + PUI.

Conclusions

NaOCl caused ultrastructural alterations in the dentin collagen, and enhanced by EDTA and/or PUI, promoted peritubular and intertubular erosion.

Clinical relevance

The effect of irrigating solutions on dentin ultrastructure was still unclear. The acknowledgment about the kind of solution, concentrations, application time, and sequence of use was important to achieve the right sanitization without jeopardizing the dentin ultrastructure quality.

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Authors and Affiliations

  1. Department of Conservative Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

    Márcia Helena Wagner, Ricardo Abreu da Rosa & Marcus Vinicius Reis Só

  2. Department of Endodontics, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil

    José Antonio Poli de Figueiredo

  3. Department of Endodontics, University of São Paulo, Bauru, SP, Brazil

    Marco Antonio Húngaro Duarte

  4. Department of Prosthodontics, University of Southern Santa Catarina, Tubarão, SC, Brazil

    Jefferson Ricardo Pereira

Authors
  1. Márcia Helena Wagner
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  2. Ricardo Abreu da Rosa
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  3. José Antonio Poli de Figueiredo
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  4. Marco Antonio Húngaro Duarte
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  5. Jefferson Ricardo Pereira
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  6. Marcus Vinicius Reis Só
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Corresponding author

Correspondence to Jefferson Ricardo Pereira.

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Conflict of interest

The authors declare that they have no conflict of interest.

Funding

The Research Vice-Deanery of the Federal University of Rio Grande do Sul (PROPESQ/UFRGS) has supported the TEM laboratory facilities and costs.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. Project n. 23051 was approved by the Committee of Research and Ethics of the Federal University of Rio Grande do Sul.

Informed consent

For this type of study, formal consent is not required.

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Wagner, M.H., da Rosa, R.A., de Figueiredo, J.A.P. et al. Final irrigation protocols may affect intraradicular dentin ultrastructure. Clin Oral Invest 21, 2173–2182 (2017). https://doi.org/10.1007/s00784-016-2006-x

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  • DOI: https://doi.org/10.1007/s00784-016-2006-x

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