Abstract
Objectives
the objective of the present exploratory study was to determine bacterial diversity and endotoxin levels in deep carious lesions of teeth presenting symptoms of reversible pulpitis.
Materials and methods
Twenty patients with deep carious lesions, reporting clinical symptomatology compatible with reversible pulpitis (n = 10) or not reporting clinical symptomatology (n = 10), were selected. Carious dentin samples were obtained with the aid of sterile and pyrogen-free spoon excavators and harvested in two steps: before and after infected dentin removal. Samples were collected for checkerboard and for kinetic chromogenic LAL assay for determination of microbial profile and quantitation of endotoxin, respectively. Data were analyzed by Mann Whitney for bacteria and two-way ANOVA for endotoxins (5%).
Results
No difference on the studied bacteria was detected between the superficial and deep dentin layers. Symptomatic teeth showed greater presence of Lactobacillus species, Capnocytophaga sputigena, and Leptotrichia buccalis. For the endotoxins, symptomatic teeth resulted in greater quantity of endotoxins (p = 0.047), being 4.13 log10 EU/mL/μg dentin and 3.45 log10 EU/mL/μg dentin, for symptomatic and asymptomatic teeth, respectively. Dentin collected in different areas presented similar number of endotoxins (p = 0.139).
Conclusion
The amount of the studied bacteria does not seem to be related to reported symptomatology of deep carious lesions, while endotoxins quantity is greater in symptomatic scenarios, regardless of the harvesting area.
Clinical relevance
The understanding of bacterial amount in reversible pulpitis is important to establish a clinical protocol of treatment.
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Acknowledgments
We are grateful for the patients’ valuable contribution during the collection of samples and for Capes, which conceived the scholarships of the MS and PhD students involved in the study.
Funding
The work was funded by the authors who received scholarships from Capes.
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The study was designed by Eduardo Bresciani and Taciana Marco Ferraz Caneppele; Eduardo Bresciani and Letícia Grilo de Souza performed the study and collected the data; Letícia Grilo de Souza, Felipe Eduardo de Oliveira, Luciane Dias de Oliveira, Claúdio Antônio Talge Carvalho, and Eduardo Bresciani processed and analyzed the data; Letícia Grilo de Souza, Manuela da Silva Spinola, Luciane Dias de Oliveira, Taciana Marco Ferraz Caneppele, and Eduardo Bresciani wrote the paper; Manuela da Silva Spinola and Eduardo Bresciani revised the paper.
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Caneppele, T.M.F., de Souza, L.G., Spinola, M.d.S. et al. Bacterial levels and amount of endotoxins in carious dentin within reversible pulpitis scenarios. Clin Oral Invest 25, 3033–3042 (2021). https://doi.org/10.1007/s00784-020-03624-7
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DOI: https://doi.org/10.1007/s00784-020-03624-7