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Osseous ingrowth in allogeneic bone blocks applied for vertical bone augmentation: a preclinical randomised controlled study

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A Correction to this article was published on 24 July 2020

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Abstract

Objectives

The aim of the present study was the qualitative and quantitative evaluation of osseous graft consolidation using allogeneic bone blocks for vertical bone augmentation in an animal model.

Material and methods

Standardised allogeneic and autologous bone blocks were fixed on the frontal skull of 20 adult female pigs and covered with a resorbable collagen membrane. Animals were sacrificed after 2 and 6 months. Specimens were histologically and histomorphometrically analysed focusing on the amount of vital bone, residual bone substitute material and connective tissue. Furthermore, the amount of expression of bone matrix proteins (collagen type I and osteocalcin) and de novo vessel formation (von Willebrand factor) were quantified by immunohistochemistry.

Results

Significantly more allogeneic bone blocks failed for both evaluation time points (p < 0.05). Allogeneic blocks showed significantly less vital bone with more connective tissue formation compared to autologous bone blocks. Increased vessel formation could be detected for both evaluation time points in the contact area of autologous bone with local bone. The expression of collagen type I and osteocalcin was significantly lower in the allogeneic bone graft.

Conclusions

Allogeneic cancellous bone blocks showed a significantly higher failure rate compared to autologous bone blocks. Allogeneic bone blocks seemed to negatively affect bone formation or negatively influence the host in the long term, and increased connective tissue formation and block loss should be anticipated.

Clinical relevance

In order to maintain patient safety and treatment success clinicians should be persuaded to make a conscious choice of the applied biomaterials with regard to their components and structure.

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Change history

  • 24 July 2020

    In the article by M��st et al., entitled ���Osseous ingrowth in allogeneic bone blocks applied for vertical bone augmentation: a preclinical randomized controlled study.

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Acknowledgements

The study was performed in cooperation with the Semmelweis-University, Budapest, Hungary. Animal care keeping and surgical procedures were performed in the European Animal Research Centre (“EARC”; 2053 Herceghalom, Hungary, Gesztenyes ut 1; Certified for “Biological evaluation of medical devices” (EN ISO 10993-2:2006). Specimen processing was undertaken in the laboratories of the Department of Oral and Maxillofacial Surgery, University of Erlangen-Nürnberg, Erlangen, Germany. Botiss biomaterials GmbH, Zossen, Germany, supported this study by providing the applied allogeneic bone blocks and membranes. The authors have no conflicts of interest. The work of Dr. Endre Felszeghy, Elke Diebel and Andrea Schönherr is highly appreciated.

Funding

This project was supported by a grant from the ITI Foundation, Switzerland.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, University of Erlangen-Nürnberg, Glueckstraße 11, 91054, Erlangen, Germany

    Tobias Moest, Johanna Frabschka, Marco Rainer Kesting, Christian Martin Schmitt, Gesche Frohwitter, Rainer Lutz & Karl Andreas Schlegel

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  1. Tobias Moest
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  2. Johanna Frabschka
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  3. Marco Rainer Kesting
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Correspondence to Tobias Moest.

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Conflicts of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national and/or institutional guidelines for the care and use of animals were followed. The study was approved by the Pest county government department for food safety and animal health, Hungary (approval number: PEI/001/961-2/2013).

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For this type of study, formal consent is not required.

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Moest, T., Frabschka, J., Kesting, M.R. et al. Osseous ingrowth in allogeneic bone blocks applied for vertical bone augmentation: a preclinical randomised controlled study. Clin Oral Invest 24, 2867–2879 (2020). https://doi.org/10.1007/s00784-019-03151-0

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