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Regulation of matrix metalloproteinase-1 by Filifactor alocis in human gingival and monocytic cells

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Abstract

Objectives

Periodontitis is a highly prevalent chronic inflammatory disease caused by periodontopathogens, such as Filifactor alocis. This study sought to examine the matrix metalloproteinase (MMP)-1 synthesis by monocytic and fibroblastic cells in response to F. alocis and to unravel the underlying cellular mechanisms.

Material and methods

Gingival biopsies from periodontally healthy and periodontitis individuals were analyzed for the presence of F. alocis and MMP-1 by RT-PCR. Human gingival fibroblastic (HGF-1) and monocytic (THP-1) cells were stimulated with F. alocis in the presence and absence of a blocking toll-like receptor (TLR)2 antibody or specific inhibitors against MAPKs. MMP-1 expression and protein levels were studied by RT-PCR and ELISA, respectively.

Results

F. alocis was highly prevalent in biopsies from periodontitis patients but barely present in the healthy gingiva. Significantly higher MMP-1 expression levels were found in the inflamed gingiva as compared with healthy biopsies. F. alocis caused a significant and dose-dependent MMP-1 upregulation in both cells. The stimulatory effect of F. alocis on MMP-1 was TLR2- and MAPK-dependent and more pronounced on THP-1 cells as compared with HGF-1 cells.

Conclusions

Our results demonstrate that F. alocis and MMP-1 are more prevalent at periodontitis sites. Additionally, our study provides original evidence that F. alocis can stimulate MMP-1 production by fibroblastic and monocytic cells, suggesting that F. alocis may contribute to periodontal breakdown through MMP-1.

Clinical relevance

F. alocis and MMP-1 are linked to each other and key players in periodontitis, which may have significant implications for future diagnostic and treatment strategies.

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Acknowledgments

The authors would like to thank Prof. Werner Götz, Ms. Ramona Menden, and Ms. Silke van Dyck for their valuable support.

Funding

This study was supported by the Medical Faculty of the University of Bonn.

Author information

Authors and Affiliations

  1. Section of Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, 53111, Germany

    Marjan Nokhbehsaim

  2. Department of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University, Augustusplatz 2, 55131, Mainz, Germany

    Andressa V. B. Nogueira, Anna Damanaki & James Deschner

  3. Department of Biological Chemistry, Medical School, National and Kapodistrian University of Athens, 157 72, Athens, Greece

    Georgia Dalagiorgou, Christos Adamopoulos, Christina Piperi, Efthimia K. Basdra & Athanasios G. Papavassiliou

  4. Department of Periodontology, Laboratory of Oral Microbiology, University of Bern, Bern, 3010, Switzerland

    Sigrun Eick

Authors
  1. Marjan Nokhbehsaim
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Contributions

Defining the study aims: James Deschner; coordination of collaboration: James Deschner; planning the experiments: Marjan Nokhbehsaim, Anna Damanaki, Christina Piperi, Efthimia K. Basdra, Athanasios G. Papavassiliou, and James Deschner; growing the bacteria and preparing the bacterial lysate: Sigrun Eick; performing preexperiments: Andressa V. B. Nogueira and Anna Damanaki; performing the experiments: Marjan Nokhbehsaim, Anna Damanaki, Georgia Dalagiorgou, and Christos Adamopoulos; monitoring/supervising the experiments: Christina Piperi, Efthimia K. Basdra, Athanasios G. Papavassiliou, and James Deschner; analyzing and discussing the data: Marjan Nokhbehsaim, Andressa V. B. Nogueira, Anna Damanaki, Georgia Dalagiorgou, Christos Adamopoulos, and James Deschner; discussing the data: Sigrun Eick, Christina Piperi, Efthimia K. Basdra, and Athanasios G. Papavassiliou; creating the figures: Marjan Nokhbehsaim, Andressa V. B. Nogueira, and James Deschner; all authors contributed to the writing of the manuscript; all authors read and approved the final manuscript.

Corresponding author

Correspondence to Andressa V. B. Nogueira.

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Research involving human participants

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Ethics Committee of the University of Bonn (043/11) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Nokhbehsaim, M., Nogueira, A.V.B., Damanaki, A. et al. Regulation of matrix metalloproteinase-1 by Filifactor alocis in human gingival and monocytic cells. Clin Oral Invest 24, 1987–1995 (2020). https://doi.org/10.1007/s00784-019-03062-0

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  • DOI: https://doi.org/10.1007/s00784-019-03062-0

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