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Gene expression of miRNA-138 and cyclin D1 in oral lichen planus

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Abstract

Objectives

This study aimed to evaluate microRNA-138 (miR-138) gene expression and its target cyclin D1 (CCND1) gene and protein expression in oral lichen planus (OLP) mucosa in an attempt to investigate their possible roles in OLP immunopathogenesis.

Methods

Sixty oral biopsy specimens were harvested from 30 healthy subjects and 30 OLP patients, subdivided into reticular, atrophic, and erosive groups (n = 10 each). Samples were subjected to quantitative real-time polymerase chain reaction analysis for quantification of miR-138 and CCND1 relative gene expression and immunohistochemical analysis to determine CCND1 protein expression.

Results

Samples from OLP patients had a significant underexpression of miR-138 gene and overexpression of CCND1 at both gene and protein levels compared to normal mucosa samples. The lowest levels of miR-138 expression were observed in atrophic and erosive OLP compared to reticular OLP, and the highest levels of CCND1 gene and protein expression were in atrophic OLP. An inverse correlation was demonstrated between the miR-138 expression and both CCND1 gene and protein expression in OLP patients. A significant positive correlation between CCND1 gene and protein expression was also observed.

Conclusion

Downregulation of miR-138 increases the gene and protein expression of its potential target CCND1 in OLP mucosa which might have a pivotal role in the disease pathogenesis.

Clinical relevance

This research implied that miR-138 may have a role in identification of symptomatic OLP lesions. MiR-138 might be considered as a potential tool in future OLP molecular therapy.

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Authors and Affiliations

  1. Department of Oral Medicine, Periodontology and Diagnosis, Faculty of Oral and Dental Medicine, Cairo University, 43 Zahraa Street, Dokki, Giza, Egypt

    Noha A. Ghallab

  2. Department of Oral Pathology, Faculty of Oral and Dental Medicine, Cairo University, Giza, Egypt

    Rehab Fawzy Kasem & Safa Fathy Abd El-Ghani

  3. Department of Medical Biochemistry& Molecular Biology, Faculty of Medicine, Cairo University, Giza, Egypt

    Olfat G. Shaker

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  1. Noha A. Ghallab
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  2. Rehab Fawzy Kasem
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  3. Safa Fathy Abd El-Ghani
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  4. Olfat G. Shaker
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Correspondence to Noha A. Ghallab.

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Conflict of interest

Dr. Noha Ghallab declares that she has no conflict of interest. Dr. Rehab Kasem declares that she has no conflict of interest. Dr. Safa Abd El-Ghani declares that she has no conflict of interest. Dr. Olfat Shaker declares that she has no conflict of interest.

Funding

The study was funded by personal resources to be refunded later by the Ministry of Higher Education, Cairo, Egypt on international publishing.

Ethical approval

This article was approved by the Faculty of Oral and Dental Medicine Research Ethics Committee, Cairo University in October 2014.

Informed consent

Following an explanation of the study as well as information about the sampling procedures, each subject signed a written informed consent form approved by the Faculty Research Ethics Committee.

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Ghallab, N.A., Kasem, R.F., El-Ghani, S.F.A. et al. Gene expression of miRNA-138 and cyclin D1 in oral lichen planus. Clin Oral Invest 21, 2481–2491 (2017). https://doi.org/10.1007/s00784-017-2091-5

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