Abstract
Background
Oral squamous cell carcinoma (OSCC) occurrence appears to be the number one among all cancers in India. Folate is a methyl donor during DNA methylation, as it provides substrate for methylenetetrahydrofolate reductase (MTHFR) to convert 5,10-MTHF to 5-MTHF and subsequently metabolizes it to methionine. The purpose of this study was to identify MTHFR C677T gene polymorphism in patients with OSCC.
Materials and methods
A total of 350 OSCC cases and 350 healthy controls participated in this study. MTHFR C677T single-nucleotide polymorphism was evaluated by PCR-RFLP.
Results
In the present study, MTHFR gene 677CC, CT, and TT genotype frequencies of the total OSCC cases were 74.8; 19.4 and 5.71; and 88.5, 9.42, and 2.0 % in controls. The average frequency of the MTHFR 677T allele was 15.4 % in OSCC cases compared to 6.71 % in the controls. The CT genotype occurrence prevailed more in patients than controls in contrast to TT genotype, although both the genotypes were statistically significant for OSCC. Moreover, we found that T allele was significant in cases of smoking and tobacco chewing.
Conclusions
In this study, we found that the homozygous mutant T allele appeared to have significantly higher risk of OSCC especially in late stages and therefore supporting in OSCC susceptibility and its progression.
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Acknowledgment
This study was supported by the Department of Biochemistry, King George’s Medical University, Lucknow, Uttar Pradesh, India.
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This study was not funded.
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The study was approved by the institutional ethical committee. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
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The study was approved by the institutional ethical committee. All participants under study signed a detailed informed consent form.
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Naqvi, H., Ahmad, M.K., Hussain, S.R. et al. Retrospective case-control study of correlation between MTHFR gene and OSCC risk in North India. Clin Oral Invest 21, 1929–1934 (2017). https://doi.org/10.1007/s00784-016-1976-z
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DOI: https://doi.org/10.1007/s00784-016-1976-z