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Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscle

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Abstract

Objectives

Periapical lesion (PL) promotes insulin resistance; however, the mechanisms underlying this alteration are not fully understood. Therefore, in this study, we aimed to evaluate the Akt serine phosphorylation status and GLUT4 expression levels in the gastrocnemius muscle (GM) of rats with PL.

Materials and methods

Male Wistar rats (n = 42) were distributed equally into control (CN) and PL groups. The pulpal tissue of the PL group rats was exposed to the oral environment for 30 days. Thereafter, glucose and insulin levels were assessed, followed by homeostasis model assessment of insulin resistance (HOMA-IR). The Akt serine phosphorylation and GLUT4 levels of microsomal (M) and plasma membrane (PM) fractions were evaluated by western blotting and analyzed statistically.

Results

Compared to CN group rats, PL group rats had lower insulin sensitivity (as observed by HOMA-IR), lower Akt serine phosphorylation status after insulin stimulus, and lower GLUT4 levels in the PM fraction. However, the M fraction in the PL group did not differ significantly from that of the CN group.

Conclusions

PL decreases insulin sensitivity, Akt phosphorylation, and PM GLUT4 content.

Clinical relevance

The present study indicates that preventing endodontic disease can thwart insulin resistance.

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Acknowledgments

This study was supported by a grant (2013/14924-0) from the São Paulo Research Foundation (FAPESP), São Paulo, SP, Brazil.

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Authors and Affiliations

  1. Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis, Department of Basic Sciences, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Rod. Marechal Rondom, km 527/528, Araçatuba, SP, 16018-805, Brazil

    Renato Felipe Pereira, Max Sander de Oliveira da Mota, Maria Sara de Lima Coutinho Mattera, Thaís Verônica Saori Tsosura, Edilson Ervolino & Doris Hissako Sumida

  2. Department of Child and Social Dentistry, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Araçatuba, SP, Brazil

    Fernando Yamamoto Chiba & Cléa Adas Saliba Garbin

  3. Endodontics, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Araçatuba, SP, Brazil

    Luciano Tavares Angelo Cintra

  4. Department of Physiology and Biophysics, São Paulo Institute of Biomedical Sciences, University of São Paulo (USP), Av. Prof. Lineu Prestes, 2415, CEP: 05508-900, São Paulo, Brazil

    Maristela Mitiko Okamoto & Ubiratan Fabres Machado

Authors
  1. Renato Felipe Pereira
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  2. Max Sander de Oliveira da Mota
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  3. Maria Sara de Lima Coutinho Mattera
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  11. Doris Hissako Sumida
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Correspondence to Doris Hissako Sumida.

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Pereira, R.F., de Oliveira da Mota, M.S., de Lima Coutinho Mattera, M.S. et al. Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscle. Clin Oral Invest 20, 1625–1630 (2016). https://doi.org/10.1007/s00784-015-1664-4

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  • DOI: https://doi.org/10.1007/s00784-015-1664-4

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