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Comparative evaluation of sealing ability and microstructure of MTA and Biodentine after exposure to different environments

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Abstract

Objectives

The aim of this study was to evaluate the sealing ability and morphological microstructure of Biodentine in comparison to ProRoot mineral trioxide aggregate (MTA) after storage in an acidic environment.

Materials and methods

Biodentine and ProRoot MTA were prepared and packed into the canal lumen of dentin disks. Twenty specimens of each material were further randomly divided into two groups according to the storage media: group A: materials with saline as storage medium; group B: materials with citric acid buffered at pH 5.4 as storage medium. The sealing ability was evaluated at 1, 3, 6, and 24 h and 1 or 3 months, using a fluid transport model for quantitative analysis of endodontic microleakage. The morphological microstructures of the materials were also evaluated using scanning electron microscopy.

Results

During the first 24 h, MTA showed greater fluid transport values than Biodentine in both environments. At the 3-month measurement, when the materials were stored in saline, MTA showed greater ability to prevent fluid movement than Biodentine (p < 0.0001). However, when the materials were stored in an acidic environment, no statistical significant difference was found after 3 months. After storage in saline, both materials showed an uneven crystalline surface with similar hexagonal crystals. The microstructure of Biodentine changed after exposure to citric acid, showing a relatively smooth surface with more spheroidal crystals.

Conclusions

The exposure to an acidic environment, within the limits of this study, seems to result in morphological changes of Biodentine in a different manner than MTA. MTA shows good ability to prevent fluid movement over time, in both environments. The ability of Biodentine to prevent fluid movement over time was enhanced in the acidic environment.

Clinical relevance

The findings of the present study could imply that both materials are indicated for use in an acidic environment.

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Acknowledgments

The authors would like to greatly acknowledge Dr. Hagay Shemesh (Chair, Division of Endodontology, Academic Centre for Dentistry Amsterdam (ACTA), the Netherlands) for his critical comments that greatly improved the manuscript.

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Authors and Affiliations

  1. Department of Endodontics, School of Dentistry, National and Kapodistrian University of Athens, 2 Thivon Str., 11527, Goudi, Athens, Greece

    Anastasia Agrafioti & Evangelos G. Kontakiotis

  2. Division of Endodontology, Department of Cariology, Endodontology and Pedodontology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University, Amsterdam, The Netherlands

    Nestor Tzimpoulas

  3. Laboratory of Mineralogy, Petrology and Economic Geology, Department of Geological Sciences, School of Mining and Metallurgical Engineering, National Technical University of Athens, Athens, Greece

    Elias Chatzitheodoridis

Authors
  1. Anastasia Agrafioti
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  2. Nestor Tzimpoulas
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  3. Elias Chatzitheodoridis
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  4. Evangelos G. Kontakiotis
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Correspondence to Evangelos G. Kontakiotis.

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Agrafioti, A., Tzimpoulas, N., Chatzitheodoridis, E. et al. Comparative evaluation of sealing ability and microstructure of MTA and Biodentine after exposure to different environments. Clin Oral Invest 20, 1535–1540 (2016). https://doi.org/10.1007/s00784-015-1638-6

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  • DOI: https://doi.org/10.1007/s00784-015-1638-6

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