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Tetracycline impregnation affects degradation of porcine collagen matrix in healthy and diabetic rats

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Abstract

Objectives

The present study evaluated the degradation of collagen matrix (CM) immersed in tetracycline (TTC) or phosphate-buffered saline (PBS) in diabetic and normoglycemic rats.

Materials and methods

Diabetes was induced in 15 rats by systemic streptozotocin (STZ) (experimental); 15 healthy rats served as controls. One day before implantation 60 CM disks, 5 mm in diameter, were labeled with biotin: 30 were immersed in tetracycline (TTC) and 30 in PBS. One disk of each type was implanted subdermally in each rat. Animals were euthanized after 3 weeks, and tissue specimens containing the disks were prepared for histologic analysis. Horseradish peroxidase (HRP)-conjugated streptavidin was used to detect the remaining biotinylated collagen. Residual collagen area within the CM disks was analyzed and compared to baseline.

Results

Diabetes significantly increased the CM degradation. Immersion of the CM disks in a 50-mg/mL TTC solution before implantation decreased its degradation both in diabetic and normoglycemic rats.

Conclusions

Diabetes significantly increases collagen matrix degradation; immersion of collagen matrix in TTC before implantation decreases its degradation in both diabetic and normoglycemic conditions.

Clinical relevance

Immersion of medical collagen devices in TTC may be an effective means to decrease their resorption rate and increase their effectiveness, especially in situations with increased degradation such as diabetes.

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Acknowledgments

This study was supported by the Gerald A Niznick Chair of Implantology at Tel Aviv University, Israel. Mucograft® was donated by Geistlich Pharma AG Wolhusen, Switzerland.

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Authors and Affiliations

  1. Department of Periodontology and Dental Implantology, Tel Aviv University School of Dental Medicine, Ramat Aviv, Tel Aviv, 69978, Israel

    Haim Tal, Asaf Shely, Carlos E. Nemcovsky & Ofer Moses

  2. Department of Oral Biology, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel

    Miron Weinreb

Authors
  1. Haim Tal
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  2. Miron Weinreb
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  3. Asaf Shely
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  4. Carlos E. Nemcovsky
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  5. Ofer Moses
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Corresponding author

Correspondence to Haim Tal.

Ethics declarations

This study was approved by Institutional Animal Care and Use Committee of the Tel Aviv University.

Conflict of interest

The authors declare that they have no competing interests.

Additional information

In partial fulfillment for the degree of D.M.D. of Asaf Shely from the Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel.

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Tal, H., Weinreb, M., Shely, A. et al. Tetracycline impregnation affects degradation of porcine collagen matrix in healthy and diabetic rats. Clin Oral Invest 20, 1237–1242 (2016). https://doi.org/10.1007/s00784-015-1615-0

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  • DOI: https://doi.org/10.1007/s00784-015-1615-0

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