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Endodontic drug delivery for root surface disinfection: a laboratory feasibility evaluation

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Abstract

Objectives

This study aims to assess the potential of a mixture of three antibiotics (TreVitaMix, TVM) as an intracanal dressing to disinfect the outer root surface by applying a new in vitro model.

Materials and methods

Fifty freshly extracted bovine roots were endodontically treated. Forty samples were then thoroughly scaled, mounted to petri dishes, gas sterilized, and randomly allocated to four groups (n = 10/group) according to their intracanal medication: sterile saline (NaCl; control, A); the TVM carrier material alone, i.e., propylene glycol (PG; B); TVM (C); and calcium hydroxide (D). In an additional group (E), the cementum was not removed and TVM was placed. Petri dishes were filled with Fastidious Anaerobe Agar, inoculated with Fusobacterium nucleatum suspension and then anaerobically incubated during 48-h intervals at 37 °C up to 192 h. Inhibition zones around the roots were then measured after each incubation period (mm2).

Results

Only teeth inoculated with the TVM dressing showed inhibition at all time points, whereas the other treatments showed no peri-radicular growing inhibition. Presence of cementum had no negative effect on disinfection (p = 0.9320).

Conclusion

TVM was able to penetrate through the dentine and inhibit the bacterial growth of F. nucleatum up to 192 h.

Clinical relevance

TVM might have the potential to sustainably disinfect the outer root surface in perio-endo lesions and serve as an adjunctive antimicrobial agent.

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Acknowledgments

We would like to thank Beatrice Sener and Andreas Meier for their help in the microbiology laboratory.

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland

    Markus Zaruba, Dan-K Rechenberg, Thomas Attin & Patrick R. Schmidlin

  2. Institute of Oral Biology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland

    Thomas Thurnheer

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  1. Markus Zaruba
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  2. Dan-K Rechenberg
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  3. Thomas Thurnheer
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  4. Thomas Attin
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Correspondence to Markus Zaruba or Patrick R. Schmidlin.

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Zaruba, M., Rechenberg, DK., Thurnheer, T. et al. Endodontic drug delivery for root surface disinfection: a laboratory feasibility evaluation. Clin Oral Invest 20, 607–613 (2016). https://doi.org/10.1007/s00784-015-1538-9

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  • DOI: https://doi.org/10.1007/s00784-015-1538-9

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