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Preoperative sterilization and disinfection of drill guide templates

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Abstract

The aim of the in vitro study was to evaluate the decontamination potential of common antiseptic solutions for heat-sensitive implantological drill guide templates. One hundred implantologists were evaluated on the basis of a questionnaire for their measures of disinfection. On the basis of these results, 80% alcohol, Octenidine 0.1%, and Chlorhexidine 0.12% were tested in an in vitro model for their decontamination efficacy for heat-sensitive plastic material infected with Pseudomonas aeruginosa, Acinetobacter baumannii, Enterococcus faecalis, Enterococcus faecium, Staphylococcus aureus, Enterobacter cloacae, Escherichia coli, and Candida albicans. The microorganisms were selected on the basis of results of environmental testing of dental laboratories. The results of the questionnaire revealed that Chlorhexidine was used by 30%, 80% alcohol by 23%, and Octenidine by 7% of the dentists. Using the in vitro model, with the exception of S. aureus, Chlorhexidine was not able to completely eliminate the microorganisms after 15 min of application. In contrast, the treatment with Octenidine revealed no further growth of the tested microorganisms after that time. The 80% alcohol was more efficient. No growth of microorganisms could be detected in any of the tests after 5 min of incubation. On the basis of our results and due to the fact that suitable installations for sterilization were hardly used by the dental practitioners, the disinfection of templates should be preferentially performed with 80% alcohol or Octenidine using an incubation time of 15 min with ultrasonication.

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References

  1. American Dental Association’s Council on Scientific Affairs and Council on Dental Practice (1996) Infection control recommendations for the dental office and the dental laboratory. J Am Dent Assoc 127:672–680

    Google Scholar 

  2. Amt für amtliche Veröffentlichungen der Europäischen Gemeinschaft, Consleg (1993) Richtlinie 93/42/ EWG des Rates vom 14. Juni 1993 über Medizinprodukte, p 1–55

  3. Arbeitskreis Krankenhaus- und Praxishygiene (2004) Hygiene in Klinik und Praxis, 3. Auflage. mhp- Verlag, Wiesbaden, p 121

    Google Scholar 

  4. Auschill TM, Hellwig E, Sculean A, Hein N, Arweiler NB (2004) Impact of the intraoral location on the rate of biofilm growth. Clin Oral Investig 8(2):97–101

    Article  PubMed  Google Scholar 

  5. Auschill TM, Hein N, Hellwig E, Follo M, Sculean A, Arweiler N, B (2005) Effect of two antimicrobial agents on early in situ biofilm formation. J Clin Periodontol 32:147–152

    Article  PubMed  Google Scholar 

  6. Bundesministerium der Justiz (Bundesministerium für Gesundheit (2001) Verordnung über Medizinprodukte (Medizinprodukte- Verordnung- MPV). BGBl I:3854, zuletzt geändert 31.10. 2006 BGBl I:2407

  7. Bundesministerium der Justiz (Bundesministerium für Gesundheit) (2002) Gesetz über Medizinprodukte (Medizinproduktegesetz- MPG), Medizinproduktegesetz in der Fassung der Bekanntmachung vom 7.8.2002. BGBl I:3146, geändert durch Artikel 145 der Verordnung vom 31.10.2006. BGBl I:2407

  8. Bundesministerium der Justiz (Bundesministerium für Gesundheit) (2002) Verordnung über das Errichten, Betreiben und Anwenden von Medizinprodukten (Medizinproduktebetreiberverordnung- MPBetreibV). BGBl I:3396, geändert durch Artikel 288 der Verordnung vom 25.11.2003. BGBl I:2340

  9. Center for Disease Control (1993) Recommended infection- control practices for dentistry. MMWR Recomm Rep 42(RR 8):1–12

    Google Scholar 

  10. Chandra J, Mukherjee PK, Leidich SD, Faddoul FF, Hoyer LL, Douglas LJ, Ghannoum MA (2001) Antifungal resistance of candidal biofilms formed on denture acrylic in vitro. J Dent Res 80:903–908

    Article  PubMed  Google Scholar 

  11. Council on Dental Therapeutics (1988) Council on dental therapeutics accepts peridex. J Am Dent Assoc 117:516–517

    Google Scholar 

  12. DePaola LG, Overholser CD, Meiller TF, Minah GE, Niehaus C (1989) Chemotherapeutic inhibition of supragingival dental plaque and gingivitis development. J Clin Periodontol 16:311–315

    Article  PubMed  Google Scholar 

  13. Deutscher Arbeitskreis für Hygiene in der Zahnmedizin (DAHZ) (2003) Hygieneleitfaden, 6. Auflage

  14. European Commission DG Enterprise (2001) Guidelines for the classification of medical devices. Medical devices Guidance document, p 1–41

  15. Fine DH, Furgang D, Lieb R, Korik I, Vincent JW, Barnett ML (1996) Effects of sublethal exposure to an antiseptic mouthrinse on representative plaque bacteria. J Clin Periodontol 23:444–451

    Article  PubMed  Google Scholar 

  16. Food and Drug Administration (2000) Guidance for industry and FDA reviewers: content and format of premarket notification submissions for liquid chemical sterilants/high level disinfectants. US Department of Health and Human Services, Food and Drug Administration, Rockville, MD

    Google Scholar 

  17. Hahn H, Falke D, Kaufmann SHE, Ullmann U (2004) Medizinische Mikrobiologie und Infektiologie, 5 Auflage. Springer, Berlin

    Google Scholar 

  18. Hahn H, Miksits K (2006) Basiswissen Medizinische Mikrobiologie und Infektiologie 3 Auflage. Springer, Berlin Heidelberg New York

    Google Scholar 

  19. Heeg P, Rehn D, Bayer U (1987) Alkohole. In: Kramer A, Weuffen W, Krasilnikow AP (eds) Handbuch der Antiseptik, Bd.II/3. Antibakterielle, antifungielle und antivirale Antiseptik- ausgewählte WirkstoffeFischer, Stuttgart, NY, pp 215–245

    Google Scholar 

  20. Heydenrijk K, Meijer HJ, van der Reijden WA, Raghoebar GM, Vissink A, Stegenga B (2002) Microbiota around root-form endosseous implants: a review of the literature. Int J Oral Maxillofac Implants 17:829–838

    PubMed  Google Scholar 

  21. Hof H (2002) Medizinische Mikrobiologie und Infektiologie, 2. Auflage. Thieme, Stuttgart NY

    Google Scholar 

  22. Hogan DA, Kolter R (2002) Pseudomonas-Candida interactions: an ecological role for virulence factors. Science 296:2229–2232

    Article  PubMed  Google Scholar 

  23. Kayser FH, Bienz KA, Eckert J, Zinkernagel RM (1998) Medizinische Mikrobiologie und Infektiologie 9 Auflage. Thieme, Stuttgart, NY

    Google Scholar 

  24. Kohn WG, Collins AS, Cleveland JL, Harte JA, Eklund KJ, Malvitz DM (2003) Guidelines for infection control in dental health-care settings—2003. MMWR Recomm Rep 52(RR 17):1–61

    PubMed  Google Scholar 

  25. Kommission für Krankenhaushygiene und Infektionsprävention beim Robert- Koch- Institut (2006) Infektionsprävention in der Zahnheilkunde- Anforderungen an die Hygiene. Bundesgesundheitsbl Gesundheitsforsch Gesundheitsschutz 4:375–394

  26. Kramer A, Hoppe H, Krull B, Pitten FA, Rosenau S (1998) Antiseptic efficacy and acceptance of Octenidin computed with common antiseptic mouthwashes. Zentralbl Hyg Umweltmed 200:443–456

    PubMed  Google Scholar 

  27. Lewis RE, Kontoyiannis DP, Darouiche RO, Raad II, Prince RA (2002) Antifungal activity of amphotericin B, fluconazole, and voriconazole in an in vitro model of Candida catheter-related bloodstream infection. Antimicrob Agents Chemother 46:3499–3505

    Article  PubMed  Google Scholar 

  28. Loe H, Schiott CR (1970) The effect of mouthrinses and topical application of chlorhexidinee on the development of dental plaque and gingivitis in man. J Periodontal Res 5:79–83

    Article  PubMed  Google Scholar 

  29. Netuschil L, Weiger R, Preisler R, Brecx M (1995) Plaque bacteria counts and vitality during chlorhexidinee, meridol and listerine mouthrinses. Eur J Oral Sci 103:355–361

    Article  PubMed  Google Scholar 

  30. Pitten FA, Kramer A (1999) Antimicrobial efficacy of antiseptic mouthrinse solutions. Eur J Clin Pharmacol 55:95–100

    Article  PubMed  Google Scholar 

  31. Pratt RJ, Pellowe CM, Wilson JA, Loveday HP, Harper PJ, Jones SR, McDougall C, Wilcox MH (2007) epic2: national evidence-based guidelines for preventing healthcare-associated infections in NHS hospitals in England. Journal Hosp Infect 65(1):1–64

    Article  Google Scholar 

  32. Ramage G, VandeWalle K, Bachmann SP, Wickes BL, Lopez-Ribot JL (2002) In vitro pharmacodynamic properties of three antifungal agents against preformed Candida albicans biofilms determined by time-kill studies. Antimicrob Agents Chemother 46:3634–3636

    Article  PubMed  Google Scholar 

  33. Schwarz F, Sculean A, Romanos G, Herten M, Horn N, Scherbaum W, Becker J (2005) Influence of different treatment approaches on the removal of early plaque biofilms and the viability of SAOS2 osteoblasts grown on titanium implants. Clin Oral Investig 9:111–117

    Article  PubMed  Google Scholar 

  34. Sennhenn-Kirchner S, Schliesing J, Jacobs HG, Mergeryan H (2004) Antimicrobial efficacy of different chemical agents on rough titanium surfaces- an in vitro study. Journal of Dental Implantology 20:152–157

    Google Scholar 

  35. Shapiro S, Giertsen E, Guggenheim B (2002) An in vitro oral biofilm model for comparing the efficacy of antimicrobial mouthrinses. Caries Res 36:93–100

    Article  PubMed  Google Scholar 

  36. Stephenson JR, Heard SR, Richards MA, Tabaqchali S (1985) Gastrointestinal colonization and septicaemia with Pseudomonas aeruginosa due to contaminated thymol mouthwash in immunocompromised patients. J Hosp Infect 6:369–378

    Article  PubMed  Google Scholar 

  37. Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV (2000) Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature 406:959–964

    Article  PubMed  Google Scholar 

  38. Suci PA, Tyler BJ (2003) A method for discrimination of subpopulations of Candida albicans biofilm cells that exhibit relative levels of phenotypic resistance to chlorhexidinee. J Microbiol Methods 53:313–325

    Article  PubMed  Google Scholar 

  39. Süßmuth R, Eberspächer J, Haag R, Springer W (1999) Biochemical-microbiological practice, 2nd edn. Thieme, Stuttgart, NY

    Google Scholar 

  40. Verran J, Kossar S, McCord JF (1996) Microbiological study of selected risk areas in dental technology laboratories. J Dent 24:77–80

    Article  PubMed  Google Scholar 

  41. Wallhäußer KH (1995) Antimikrobielle Wirkstoffe. In: Praxis der Sterilisation, Desinfektion, Konservierung: Keimidentifikation- Betriebshygiene. Georg Thieme, Stuttgart, New York, pp 578–579

  42. Wennemar M (2004) Bakterielle Kontamination bei Implantation von Knie- und Hüftendoprothesen:, med. Diss. Universität Marburg

  43. Zablotsky M, Meffert R, Mills O, Burgess A, Lancaster D (1992) The macroscopic, microscopic and spectrometric effects of various chemotherapeutic agents on the plasma-sprayed hydroxyapatite-coated implant surface. Clin Oral Implants Res 3:189–198

    Article  PubMed  Google Scholar 

  44. Zablotsky MH, Diedrich DL, Meffert RM (1992) Detoxification of endotoxin-contaminated titanium and hydroxyapatite-coated surfaces utilizing various chemotherapeutic and mechanical modalities. Implant Dent 1:154–158

    Article  PubMed  Google Scholar 

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Acknowledgements

The authors would like to express their appreciation to Cyrilla Maelicke for critical review of the manuscript and linguistic assistance and to Claudia Huppach for her kind support in the laboratory work. This work comprises parts of the doctoral thesis of S. Weustermann.

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Authors and Affiliations

  1. Department of Oral Surgery, Georg-August University, Robert-Koch-Str. 40, 37075, Göttingen, Germany

    Sabine Sennhenn-Kirchner, Hans Georg Jacobs & Bernhard Kirchner

  2. Department of Prosthodontics, Georg-August University, Göttingen, Germany

    Sascha Weustermann

  3. Department of Hospital Hygiene and Infection Control, Georg-August University, Göttingen, Germany

    Hamparsum Mergeryan

  4. Institute of Medical Microbiology, Georg-August University, Göttingen, Germany

    Margarete Borg- von Zepelin

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  1. Sabine Sennhenn-Kirchner
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  2. Sascha Weustermann
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Correspondence to Sabine Sennhenn-Kirchner.

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Sennhenn-Kirchner, S., Weustermann, S., Mergeryan, H. et al. Preoperative sterilization and disinfection of drill guide templates. Clin Oral Invest 12, 179–187 (2008). https://doi.org/10.1007/s00784-007-0153-9

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