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Levels of parotid and submandibular/sublingual salivary immunoglobulin A in response to experimental gingivitis in humans

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Abstract

Salivary secretory IgA (s-IgA) is considered to act as an important first line of defense mechanism in the oral cavity. It has therefore been suggested that an increased antigenic load would induce an increase in salivary IgA production. This study investigated the pure glandular levels of salivary IgA in parotid and submandibular/sublingual (SM/SL) saliva during plaque accumulation leading to experimental gingivitis. Starting from regular oral hygiene, 14 healthy, nonsmoking men refrained from all oral hygiene measures for 12 days. On days −2, 0, 3, 6, and 12 a plaque index, a bleeding index, and unstimulated and stimulated saliva from the parotid and the SM/SL glands were measured. Salivary IgA was quantified using a sandwich ELISA. All subjects developed gingivitis as measured by a bleeding index. Compared to baseline the salivary flow rate was increased on day 12. Regarding the secretion rate of IgA there was a statistically significant increase in stimulated parotid saliva but not SM/SL saliva compared to baseline after 6 and 12 days without oral hygiene. No significant changes were observed for the concentration of IgA during the trial. Thus, in healthy subjects with regular oral hygiene the development of plaque induced gingivitis is associated with increased salivary gland output and increased total IgA output levels in stimulated parotid saliva but not in SM/SL saliva.

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References

  1. Brandtzaeg P (1971) Human secretory immunoglobulins. VII. Concentrations of parotid IgA and other secretory proteins in relation to the rate of flow and duration of secretory stimulus. Arch Oral Biol 16:1295–1310

    Article  CAS  PubMed  Google Scholar 

  2. Brandtzaeg P (1989) Salivary immunoglobulins. In: Tenovuo J (ed) Human saliva: clinical chemistry and microbiology. CRC, Boca Raton, pp 2–54

  3. Dimitriou L, Sharp NC, Doherty M (2002) Circadian effects on the acute responses of salivary cortisol and IgA in well trained swimmers. Br J Sports Med 36:260–264

    Article  CAS  PubMed  Google Scholar 

  4. Emmings FG, Evans RT, Genco RJ (1975) Antibody response in the parotid fluid and serum of irus monkeys (Macaca fascicularis) after local immunization with Streptococcus mutans. Infect Immun 12:281–292

    CAS  PubMed  Google Scholar 

  5. Garrett JR, Proctor GB (1998) Control of salivation. In: Linden RWA (ed) The scientific basis of eating. Frontal oral biology. Karger, Basel, pp 135–155

  6. Gomez E, Ortiz V, Saint-Martin B, Boeck L, Diaz-Sanchez V, Bourges H (1993) Hormonal regulation of the secretory IgA (sIgA) system: estradiol- and progesterone-induced changes in sIgA in parotid saliva along the menstrual cycle. Am J Reprod Immunol 29:219–223

    CAS  PubMed  Google Scholar 

  7. Hagewald S, Bernimoulin JP, Kottgen E, Kage A (2000) Total IgA and Porphyromonas gingivalis-reactive IgA in the saliva of patients with generalised early-onset periodontitis. Eur J Oral Sci 108:147–153

    Article  CAS  PubMed  Google Scholar 

  8. Harding J, Berry WC, Marsh C, Jolliff CR (1980) Salivary antibodies in acute gingivitis. J Periodontol 51:63–69

    CAS  PubMed  Google Scholar 

  9. Henskens YM, van der Weijden FA, van den Keijbus PA, Veerman EC, Timmerman MF, van der Velden U, Amerongen AV (1996) Effect of periodontal treatment on the protein composition of whole and parotid saliva. J Periodontol 67:205–212

    CAS  PubMed  Google Scholar 

  10. Jalil RA, Ashley FP, Wilson RF (1992) The relationship between 48-h dental plaque accumulation in young human adults and the concentrations of hypothiocyanite, ‘free’ and ‘total’ lysozyme, lactoferrin and secretory immunoglobulin A in saliva. Arch Oral Biol 37:23–28

    Article  CAS  PubMed  Google Scholar 

  11. Jalil RA, Ashley FP, Wilson RF, Wagaiyu EG (1993) Concentrations of thiocyanate, hypothiocyanite, ‘free’ and ‘total’ lysozyme, lactoferrin and secretory IgA in resting and stimulated whole saliva of children aged 12–14 years and the relationship with plaque accumulation and gingivitis. J Periodontal Res 28:130–136

    CAS  PubMed  Google Scholar 

  12. Kalk WW, Vissink A, Spijkervet FK, Bootsma H, Kallenberg CG, Nieuw Amerongen AV (2001) Sialometry and sialochemistry: diagnostic tools for Sjogren’s syndrome. Ann Rheum Dis 60:1110–1116

    Article  CAS  PubMed  Google Scholar 

  13. Kalk WW, Vissink A, Stegenga B, Bootsma H, Nieuw Amerongen AV, Kallenberg CG (2002) Sialometry and sialochemistry: a non-invasive approach for diagnosing Sjogren’s syndrome. Ann Rheum Dis 61:137–144

    Article  CAS  PubMed  Google Scholar 

  14. Kilian M, Mestecky J, Russell MW (1988) Defense mechanisms involving Fc-dependent functions of immunoglobulin A and their subversion by bacterial immunoglobulin A proteases. Microbiol Rev 52:296–303

    CAS  PubMed  Google Scholar 

  15. Kinane DF, Lappin DF (2001) Clinical, pathological and immunological aspects of periodontal disease. Acta Odontol Scand 59:154–160

    Article  CAS  PubMed  Google Scholar 

  16. Korsrud FR, Brandtzaeg P (1980) Quantitative immunohistochemistry of immunoglobulin- and J-chain-producing cells in human parotid and submandibular salivary glands. Immunology 39:129–140

    CAS  PubMed  Google Scholar 

  17. Lashley KS (1916) Reflex secretion of the human parotid gland. J Exp Psychol 16:461–493

    Google Scholar 

  18. Lie MA, Myint MM, Schenck K, Timmerman MF, van der Velden U, van der Weijden GA, Loos BG (2002) Parotid salivary S-IgA antibodies during experimental gingivitis in smokers and non-smokers. J Periodontal Res 37:86–92

    Article  CAS  PubMed  Google Scholar 

  19. Lindstrom FD, Folke LE (1973) Salivary IgA in periodontal disease. Acta Odontol Scand 31:31–34

    CAS  PubMed  Google Scholar 

  20. Löe H, Theilade E, Jensen SB (1965) Experimental gingivitis in man. J Periodontol 36:177–187

    PubMed  Google Scholar 

  21. Macpherson AJ, Gatto D, Sainsbury E, Harriman GR, Hengartner H, Zinkernagel RM (2000) A primitive T cell-independent mechanism of intestinal mucosal IgA responses to commensal bacteria. Science 288:2222–2226

    Article  CAS  PubMed  Google Scholar 

  22. Macpherson AJ, Lamarre A, McCoy K, Harriman GR, Odermatt B, Dougan G, Hengartner H, Zinkernagel RM (2001) IgA production without mu or delta chain expression in developing B cells. Nat Immunol 2:625–631

    Article  CAS  PubMed  Google Scholar 

  23. Marcotte H, Lavoie MC (1998) Oral microbial ecology and the role of salivary immunoglobulin A. Microbiol Mol Biol Rev 62:71–109

    Google Scholar 

  24. McGhee JR, Kiyono H (1994) Effective mucosal immunity. Current concepts for vaccine delivery and immune response analysis. Int J Technol Assess Health Care 10:93–106

    CAS  PubMed  Google Scholar 

  25. McGhee JR, Mestecky J, Dertzbaugh MT, Eldridge JH, Hirasawa M, Kiyono H (1992) The mucosal immune system: from fundamental concepts to vaccine development. Vaccine 10:75–88

    Article  CAS  PubMed  Google Scholar 

  26. McNabb PC, Tomasi TB (1981) Host defense mechanisms at mucosal surfaces. Annu Rev Microbiol 35:477–496

    Article  CAS  PubMed  Google Scholar 

  27. Michalek SM, Katz J, Childers NK (2001) A vaccine against dental caries: an overview. BioDrugs 15:501–508

    CAS  PubMed  Google Scholar 

  28. Myint MM, Steinsvoll S, Odden K, Dobloug J, Schenck K (1997) Salivary IgA responses to bacteria in dental plaque as related to periodontal and HIV infection status. Eur J Oral Sci 105:562–570

    Google Scholar 

  29. Nair PN, Schroeder HE (1983) Local immune response to repeated topical antigen application in the simian labial mucosa. Infect Immun 41:399–409

    CAS  PubMed  Google Scholar 

  30. Nair PN, Schroeder HE (1983) Retrograde access of antigens to the minor salivary glands in the monkey Macaca fascicularis. Arch Oral Biol 28:145–152

    Article  CAS  PubMed  Google Scholar 

  31. Oon CH, Lee J (1972) A controlled quantitative study of parotid salivary secretory IgA-globulin in normal adults. J Immunol Methods 2:45–48

    Article  CAS  PubMed  Google Scholar 

  32. Orstavik D, Brandtzaeg P (1975) Secretion of parotid IgA in relation to gingival inflammation and dental caries experience in man. Arch Oral Biol 20:701–704

    Article  CAS  PubMed  Google Scholar 

  33. Paster BJ, Boches SK, Galvin JL, Ericson RE, Lau CN, Levanos VA, Sahasrabudhe A, Dewhirst FE (2001) Bacterial diversity in human subgingival plaque. J Bacteriol 183:3770–3783

    Article  CAS  PubMed  Google Scholar 

  34. Quigley GA, Hein JW (1962) Comparative cleansing efficiency of manual and power brushing. J Am Dent Assoc 65:26

    CAS  PubMed  Google Scholar 

  35. Ranney RR, Ruddy S, Tew JG, Welshimer HJ, Palcanis KG, Segreti A (1981) Immunological studies of young adults with severe periodontitis. I. Medical evaluation and humoral factors. J Periodontal Res 16:390–402

    CAS  PubMed  Google Scholar 

  36. Roitt IM, Lehner T (1983) Immune responses to dental bacterial plaque. In: Roitt IM, Lehner T (eds) Immunology of oral diseases. Blackwell Scientific, London, pp 305–323

  37. Rudney JD, Krig MA, Neuvar EK, Soberay AH, Iverson L (1991) Antimicrobial proteins in human unstimulated whole saliva in relation to each other, and to measures of health status, dental plaque accumulation and composition. Arch Oral Biol 36:497–506

    Article  CAS  PubMed  Google Scholar 

  38. Sandholm L, Gronblad E (1984) Salivary immunoglobulins in patients with juvenile periodontitis and their healthy siblings. J Periodontol 55:9–12

    CAS  PubMed  Google Scholar 

  39. Saxer UP, Muhlemann HR (1975) Motivation and education. SSO Schweiz Monatsschr Zahnheilkd 85:905–919

    CAS  PubMed  Google Scholar 

  40. Schenck K, Poppelsdorf D, Denis C, Tollefsen T (1993) Levels of salivary IgA antibodies reactive with bacteria from dental plaque are associated with susceptibility to experimental gingivitis. J Clin Periodontol 20:411–417

    CAS  PubMed  Google Scholar 

  41. Stuchell RN, Mandel ID (1978) Studies of secretory IgA in caries-resistant and caries-susceptible adults. Adv Exp Med Biol 107:341–348

    CAS  PubMed  Google Scholar 

  42. Talham GL, Jiang HQ, Bos NA, Cebra JJ (1999) Segmented filamentous bacteria are potent stimuli of a physiologically normal state of the murine gut mucosal immune system. Infect Immun 67:1992–2000

    CAS  PubMed  Google Scholar 

  43. Van der Velden U, Abbas F, Winkel EG (1986) Probing considerations in relation to susceptibility to periodontal breakdown. J Clin Periodontol 13:894–899

    PubMed  Google Scholar 

  44. Walker J (1981) Antibody responses of monkeys to oral and local immunization with Streptococcus mutans. Infect Immun 31:61–70

    CAS  PubMed  Google Scholar 

  45. Wolf RO (1964) Regulated vacuum system for collecting submaxillary and sublingual saliva. J Dent Res 43:303–306

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Operative and Preventive Dentistry, Virchow Campus, Charité University Medical School of Berlin, Augustenburger Platz 1, 13353, Berlin, Germany

    R. Seemann, V. Sztankay, J. Drews & M. Bizhang

  2. Department of Periodontology and Synoptic Dentistry, Virchow Campus, Charité University Medical School of Berlin, Augustenburger Platz 1, 13353, Berlin, Germany

    S. J. Hägewald

  3. Institute of Laboratory Medicine and Pathobiochemistry, Virchow Campus, Charité University Medical School of Berlin, Augustenburger Platz 1, 13353, Berlin, Germany

    A. Kage

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  1. R. Seemann
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  2. S. J. Hägewald
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Correspondence to R. Seemann.

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Seemann, R., Hägewald, S.J., Sztankay, V. et al. Levels of parotid and submandibular/sublingual salivary immunoglobulin A in response to experimental gingivitis in humans. Clin Oral Invest 8, 233–237 (2004). https://doi.org/10.1007/s00784-004-0280-5

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  • DOI: https://doi.org/10.1007/s00784-004-0280-5

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