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CircPVT1 promotes the tumorigenesis and metastasis of osteosarcoma via mediation of miR-26b-5p/CCNB1 axis

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Journal of Bone and Mineral Metabolism Aims and scope Submit manuscript

Abstract

Introduction

Osteosarcoma (OS) is the most aggressive malignancy among the bone tumors in the world. Circular RNAs (circRNAs) have been reported to be participated in multiple cancers, including OS. Meanwhile, circPVT1 has been proved to be upregulated in OS. However, the mechanism by which circPVT1 mediates the tumorigenesis of OS remains to be further explored.

Materials and methods

Protein and gene expressions in OS cells were measured by western blot and RT-qPCR, respectively. Cell growth was assessed by flow cytometry and colony formation, respectively. In addition, cell migration was assessed by wound healing, and invasion was evaluated by Transwell assay. Meanwhile, the correlation among circPVT1, miR-26b-5p and CCNB1 was explored by RNA pull-down and dual luciferase assay. Finally, in vivo model was established to explore the role of circPVT1 in OS in vivo.

Results

CircPVT1 and CCNB1 were significantly upregulated in OS cells, while miR-26b-5p was downregulated. Knockdown of circPVT1 notably inhibited proliferation and induced apoptosis of OS cells. CircPVT1 shRNA significantly suppressed the OS cell invasion and migration. Meanwhile, circPVT1 sponged miR-26b-5p and CCNB1 was found to be the direct target of miR-26b-5p. Furthermore, silencing of circPVT1 inhibited the growth and metastasis of OS in vivo.

Conclusion

Silencing of circPVT1 notably suppressed the tumorigenesis and metastasis of OS via miR-26b-5p/CCNB1 axis. Therefore, circPVT1 might be used as a target for OS treatment.

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Abbreviations

CircPVT1:

Circular PVT1

CCNB1:

Cyclin B1

H&E:

Hematoxylin–eosin

OS:

Osteosarcoma

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Author information

Authors and Affiliations

  1. Department of Pediatric Orthopedics, Hunan Children’s Hospital, No. 86, Ziyuan Road, Changsha, 410007, Hunan, People’s Republic of China

    Sheng-Xiang Huang, Hai-Bo Mei, Kun Liu, Jin Tang, Jiang-Yan Wu, Guang-Hui Zhu & Wei-Hua Ye

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  1. Sheng-Xiang Huang
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  2. Hai-Bo Mei
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  3. Kun Liu
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  4. Jin Tang
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  6. Guang-Hui Zhu
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Contributions

S-XH: conceptualization, methodology, and writing—original draft preparation; H-BM: visualization and writing—original draft preparation; KL: data curation; JT: data curation; J-YW: investigation; G-HZ: validation; W-HY: conceptualization, supervision, and writing—reviewing and editing.

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Correspondence to Wei-Hua Ye.

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Conflict of interest

The authors declare that they have no competing interest.

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This study was performed according to the guideline of National Institutes of Health and approved by the Ethics Committees of Hunan Children’s Hospital.

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Supplementary Information

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Supplementary Fig. 1 CircPVT1 level was negatively correlated with miR-26b-5p and positively correlated with CCNB1. (A, B, C)

The levels of circPVT1, miR-26b-5p and CCNB1 in OS and normal tissues were detected by RT-qPCR. (D) K-M analysis was performed to detect the correlation between circPVT1 and miR-26b-5p/CCNB1. All the results were shown as mean ± SD (n = 3), which were three different experiments performed in triplicate. ***P < 0.001.upplementary file1 (TIF 4878 kb)

Supplementary Fig. 2 CircPVT1 mediated miR-26b-5p/CCNB1 axis in OS cells.

(A) OS cells were transfected with inhibitor NC or miR-26b-5p inhibitor. The level of circPVT1 in OS cells was detected by RT-qPCR. (B) OS cells were transfected with shNC or shCCNB1. The level of circPVT1 in OS cells was detected by RT-qPCR. (C) OS cells were transfected with NC, shcircPVT1, miR-26b-5p inhibitor or shcircPVT1 + miR-26b-5p inhibitor. The viability of OS cells was tested by CCK-8 assay. (D) The proliferation of OS cells was evaluated by colony formation assay. (E) The migration of OS cells was investigated by wound healing assay. (F) The invasion of OS cells was tested by Transwell assay. All the results were shown as mean ± SD (n = 3), which were three different experiments performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001.upplementary file2 (TIF 26761 kb)

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Huang, SX., Mei, HB., Liu, K. et al. CircPVT1 promotes the tumorigenesis and metastasis of osteosarcoma via mediation of miR-26b-5p/CCNB1 axis. J Bone Miner Metab 40, 581–593 (2022). https://doi.org/10.1007/s00774-022-01326-6

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  • DOI: https://doi.org/10.1007/s00774-022-01326-6

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