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Effects of mild hyperbaric oxygen on osteoporosis induced by hindlimb unloading in rats

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Abstract

Introduction

Disuse-induced bone loss is caused by a suppression of osteoblastic bone formation and an increase in osteoclastic bone resorption. There are few data available for the effects of environmental conditions, i.e., atmospheric pressure and/or oxygen concentration, on osteoporosis. This study examined the effects of mild hyperbaric oxygen at 1317 hPa with 40% oxygen on unloading-induced osteoporosis.

Materials and methods

Eighteen 8-week old male Wistar rats were randomly divided into three groups: the control for 21 days without unloading and mild hyperbaric oxygen (NOR, n = 6), the unloading for 21 days and recovery for 10 days without mild hyperbaric oxygen (HU + NOR, n = 6), and the unloading for 21 days and recovery for 10 days with mild hyperbaric oxygen (HU + MHO, n = 6).

Results

The cortical thickness and trabecular bone surface area were decreased in the HU + NOR group compared to the NOR group. There were no differences between the NOR and HU + MHO groups. Osteoclast surface area and Sclerostin (Sost) mRNA expression levels were decreased in the HU + MHO group compared to the HU + NOR group. These results suggested that the loss of the cortical and trabecular bone is inhibited by mild hyperbaric oxygen, because of an inhibition of osteoclasts and enhancement of bone formation with decreased Sost expression.

Conclusions

We conclude that exposure to mild hyperbaric oxygen partially protects from the osteoporosis induced by hindlimb unloading.

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Acknowledgements

This work was supported by the Japan society for the promotion of science (Project number 17J02040). The authors are grateful to Dr. Takahiro Yamashita for technical assistance with the experiments.

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Author notes

  1. Ai Takemura

    Present address: Department of Sports Research, Japan Institute of Sport Sciences, Tokyo, 115-0056, Japan

Authors and Affiliations

  1. Laboratory of Cell Biology and Life Science, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto, 606-8501, Japan

    Ai Takemura & Akihiko Ishihara

  2. Department of Translational Dental Medicine, Boston University Goldman School of Dental Medicine, Boston, MA, 02118, USA

    Paola Divieti Pajevic

  3. Laboratory of Sports and Exercise Medicine, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto, 606-8501, Japan

    Tatsuro Egawa & Tatsuya Hayashi

  4. Laboratory of Developmental Epigenome, Graduate School of Medicine, Kyoto University, Kyoto, 606-8507, Japan

    Rika Teshigawara

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Correspondence to Ai Takemura.

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This study was approved by the Ethics Committee for Human and Animal Research of the Graduate School of Human and Environmental Studies of Kyoto University (Approval number: 30-A-4). All experimental and animal care procedures were conducted in accordance with the Guiding Principles for the Care and Use of Animals in the Field of Physiological Science (The Physiological Society of Japan, 2015).

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Takemura, A., Pajevic, P.D., Egawa, T. et al. Effects of mild hyperbaric oxygen on osteoporosis induced by hindlimb unloading in rats. J Bone Miner Metab 38, 631–638 (2020). https://doi.org/10.1007/s00774-020-01100-6

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  • DOI: https://doi.org/10.1007/s00774-020-01100-6

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