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Soybean isoflavones preserve bone mass in hindlimb-unloaded mice

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Abstract

It is well known that skeletal unloading induces bone loss. It has been shown that soybean isoflavones are effective in preventing bone loss in osteoporotic animals. We investigated the ability of isoflavones to protect bone loss induced by hindlimb unloading by using a tail-suspension mouse model. Eight-week-old female mice were divided into five groups: (1) normal housed group (Normal), (2) sham unloaded group fed a control diet (Sham-UL), (3) hindlimb unloaded group fed a control diet (UL-C), (4) hindlimb unloaded group fed a 0.25% isoflavone conjugates diet (UL-ISO 0.25), and (5) hindlimb unloaded group fed a 0.5% isoflavone conjugates diet (UL-ISO 0.5). After 3 weeks, bone mineral density (BMD) of the femur was significantly decreased in UL-C, and this bone loss was prevented by isoflavone treatment. Histomorphometric analysis revealed a decrease in the cancellous bone of the distal femur in the UL-C group, and isoflavone prevented this change. Serum corticosterone increased in the UL-C group, and isoflavones inhibited the elevation. These results suggest that isoflavones might be promising food components that provide protection from bone loss and normalize stress-induced serum corticosterone during skeletal unloading.

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Authors and Affiliations

  1. Division of Applied Food Research, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8636, Japan

    Fumie Sugiyama, Jian Wu, Maiko Fujioka, Junko Ezaki, Tatsuya Ishida, Kazuhiko Yamada & Yoshiko Ishimi

  2. Department of Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan

    Fumie Sugiyama, Maiko Fujioka & Ken Takeda

  3. Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan

    Chisato Miyaura

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  1. Fumie Sugiyama
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  2. Jian Wu
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  3. Maiko Fujioka
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  4. Junko Ezaki
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  5. Ken Takeda
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  6. Chisato Miyaura
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  7. Tatsuya Ishida
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  8. Kazuhiko Yamada
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  9. Yoshiko Ishimi
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Correspondence to Yoshiko Ishimi.

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Sugiyama, F., Wu, J., Fujioka, M. et al. Soybean isoflavones preserve bone mass in hindlimb-unloaded mice. J Bone Miner Metab 24, 439–446 (2006). https://doi.org/10.1007/s00774-006-0711-2

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  • DOI: https://doi.org/10.1007/s00774-006-0711-2

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