Abstract
The production of a simulated urine sample is necessary for external quality assessment schemes in clinical testing. This study aimed to optimize the formulation of a simulated urine sample containing patient urine bacterial Enterococcus faecalis via response surface methodology based on central composite design. The results of a Plackett–Burman design experiment showed that three factors including sodium thioglycolate and peptone concentration and pH value significantly effected to concentration of E. faecalis. Notably, an initial concentration of 1.51 mg/mL of sodium thioglycolate and 3.63 mg/mL of peptone at a pH value of 8.06 of culture medium has been found to maximize the E. faecalis concentration (7.05 log (CFU/mL)). This optimal condition could be applied for production of a simulated urine sample containing E. faecalis.
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References
Whitehead TP, Woodford FP (1981) External quality assessment of clinical laboratories in the United Kingdom. J Clin Pathol 34:947–957
Sciacovelli L, Secchiero S, Zardo L, Zaninotto M, Plebani M (2006) External quality assessment: an effective tool for Clinical Governance in laboratory medicine. Clin Chem Lab Med 44:740–749
Sciacovelli L, Secchiero S, Zardo L, Plebani M (2010) The role of the external quality assessment. Biochemia Medica 20:160–164
Gulhan T, Boynukara B, Ciftci A, Sogut MU, Findik A (2015) Characterization of Enterococcus faecalis isolates originating from different sources for their virulence factors and genes, antibiotic resistance patterns, genotypes and biofilm production. Iran J Vet Res 16:261–266
Plackett RL, Burman JP (1946) The design of optimum multifactorial experiments. Biometrika 33:305–325
Lesmana M, Salim OC, Herwana E, Bukitwetan P, Surjawidjaja JE (2008) Comparative analysis of transport media for isolating Shigella. Universa medicina 27:51–56
Momtaz W, Buhari O, Atef E, Albert C (1995) Comparison of preservation media for storage of stool samples. J Clin Microbiol 33:2176–2178
Enrique DC (2007) Process optimization: a statistical approach. Springer, Germany
Stieglmeier M, Wirth R, Kminek G, Moissl-Eichinger C (2009) Cultivation of anaerobic and facultatively anaerobic bacteria from spacecraft-associated clean rooms. Appl Environ Microbiol 75:3484–3491
Gray VL, Müller CT, Watkins ID, Lloyd D (2008) Peptones from diverse sources: pivotal determinants of bacterial growth dynamics. J Appl Microbiol 104:554–565
Jin Q, Kirk MF (2018) pH as a primary control in environmental microbiology: 1. Thermodynamic perspective. Front Environ Sci 6:1–15
Acknowledgements
This study was planned collaboratively by: (1). Center for Standardization and Quality control in medical Lab of HCMC; (2). Department of Science and Technology of HCMC.
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Tran, H.T., Vo, N.N. & Truong, Q.T. Optimization of medium composition for production of a simulated urine sample containing Enterococcus faecalis. Accred Qual Assur 27, 119–124 (2022). https://doi.org/10.1007/s00769-022-01493-z
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DOI: https://doi.org/10.1007/s00769-022-01493-z