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Evaluation of measurement uncertainty in the determination of jasmonic acid in Lemna minor L. by liquid chromatography with fluorescence detection

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Abstract

The uncertainty was estimated for the determination of jasmonic acid (JA) content in Lemna minor L. plant extracts using a reverse-phase liquid chromatography with fluorescence detection. JA was extracted from plant material, followed by solid-phase extraction procedures, derivatisation and quantification. In the estimation of uncertainty, the sampling, sample processing and chromatographic determination that may significantly influence the uncertainty of analytical data were considered. The results show that the method recovery and sample homogeneity are the two main contributors to uncertainty. The method has a relative expanded uncertainty (coverage factor k = 2) of about 17% at the JA content of approximately 100 ng/g.

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Acknowledgements

The authors gratefully acknowledge the financial support from the Slovenian Research Agency (P1-0164, P1-153).

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Authors and Affiliations

  1. Faculty of Agriculture, University of Maribor, Vrbanska 30, Maribor, 2000, Slovenia

    Janja Kristl & Božidar Krajnčič

  2. Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova 17, Maribor, 2000, Slovenia

    Darinka Brodnjak-Vončina

  3. Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, Ljubljana, 1000, Slovenia

    Marjan Veber

Authors
  1. Janja Kristl
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  2. Božidar Krajnčič
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  3. Darinka Brodnjak-Vončina
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  4. Marjan Veber
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Correspondence to Janja Kristl.

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Kristl, J., Krajnčič, B., Brodnjak-Vončina, D. et al. Evaluation of measurement uncertainty in the determination of jasmonic acid in Lemna minor L. by liquid chromatography with fluorescence detection. Accred Qual Assur 12, 303–310 (2007). https://doi.org/10.1007/s00769-007-0271-8

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  • DOI: https://doi.org/10.1007/s00769-007-0271-8

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