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Modulation of muscle protein synthesis by amino acids: what consequences for the secretome? A preliminary in vitro study

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Abstract

The modulation by amino acids of muscle secretome is largely unknown. In this study, we investigate the effect of hyperaminoacidemia or specific amino acids (citrulline or leucine) on protein synthesis and secretome in myotubes. All conditions stimulate muscle protein synthesis, and secretome is differently modulated depending of the amino acids considered. In conclusion, the activation of protein synthesis by amino acids induces different modulations of the muscle secretome, proposing a new role of amino acids in the regulation of muscle function.

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Abbreviations

AA:

Amino acid

CIT:

Citrulline

LEU:

Leucine

MPS:

Muscle protein synthesis

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Funding

A. Goron was supported by a grant from Agir pour les maladies chroniques. C. Breuillard and this work were supported by the UGA Foundation (“Chaire Nutrition des Séniors”) thanks to the sponsorship of Findus.

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Authors and Affiliations

  1. Université de Grenoble Alpes, LBFA et BEeSy, INSERM U1055, PROMETHEE Proteomic Platform, 2280 rue de la Piscine, BP 53, 38041, Grenoble, France

    Arthur Goron, Charlotte Breuillard, Valérie Cunin, Sandrine Bourgoin-Voillard, Michel Seve & Christophe Moinard

  2. CHU Grenoble Alpes, PROMETHEE Proteomic Platform, Institut de Biologie et de Pathologie, Grenoble, France

    Valérie Cunin, Sandrine Bourgoin-Voillard & Michel Seve

Authors
  1. Arthur Goron
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  2. Charlotte Breuillard
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  3. Valérie Cunin
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  4. Sandrine Bourgoin-Voillard
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  5. Michel Seve
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  6. Christophe Moinard
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Contributions

AG, CB contributed to all parts of this work. VC contributed to the experimental proteomics part of this work. SB-V and MS contributed to the analytic proteomics part of this work. CM contributed to the lead of this work and the redaction of the article.

Corresponding author

Correspondence to Christophe Moinard.

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Conflict of interest

C. Moinard and C. Breuillard are shareholders in the Citrage company. Other authors have no conflicts of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.

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Handling Editor: F. Blachier.

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Goron, A., Breuillard, C., Cunin, V. et al. Modulation of muscle protein synthesis by amino acids: what consequences for the secretome? A preliminary in vitro study. Amino Acids 51, 1681–1688 (2019). https://doi.org/10.1007/s00726-019-02796-1

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  • DOI: https://doi.org/10.1007/s00726-019-02796-1

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