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Proteome changes during yeast-like and pseudohyphal growth in the biofilm-forming yeast Pichia fermentans

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Abstract

The Pichia fermentans strain DISAABA 726 is a biofilm-forming yeast that has been proposed as biocontrol agent to control brown rot on apple. How ever, when inoculated on peach, strain 726 shows yeast-like to pseudohyphal transition coupled to a pathogenic behaviour. To identify the proteins potentially involved in such transition process, a comparative proteome analysis of P. fermentans 726 developed on peach (filamentous growth) vs apple (yeast-like growth) was carried out using two-dimensional gel electrophoresis coupled with mass spectrometry analysis. The proteome comparison was also performed between the two different cell morphologies induced in a liquid medium amended with urea (yeast-like cells) or methionine (filamentous cells) to exclude fruit tissue impact on the transition. Seventy-three protein spots showed significant variations in abundance (±twofold, p < 0.01, confidence intervals 99 %) between pseudohyphal vs yeast-like morphology produced on fruits. Among them, 30 proteins changed their levels when the two morphologies were developed in liquid medium. The identified proteins belong to several pathways and functions, such as glycolysis, amino acid synthesis, chaperones, and signalling transduction. The possible role of a group of proteins belonging to the carbohydrate pathway in the metabolic re-organisation during P. fermentans dimorphic transition is discussed.

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Acknowledgments

We are grateful to Dr. Stefano Fiori for carrying out the experiments on apple and peach fruits. This work has been granted by Ministry of Instruction and Research (MIUR) of Italy by PRIN07 project: evaluation of new risk factors associated with the utilisation of microbial antagonists.

Conflict of interest

The authors declare that they have no competing financial interest.

Author information

Authors and Affiliations

  1. CNR-IPSP, Consiglio Nazionale delle Ricerche-Dipartimento di Scienze Bio-Agroalimentari, Istituto per la Protezione Sostenibile delle Piante, Area della Ricerca CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Florence, Italy

    Biancaelena Maserti & Alessandra Podda

  2. CNR-IBBA, Consiglio Nazionale delle Ricerche-Dipartimento di Scienze Bio-Agroalimentari, Istituto di Biologia e Biotecnologia Agraria, Area della Ricerca CNR, Via Moruzzi 1, 56124, Pisa, Italy

    Lucia Giorgetti

  3. CNR-IFC, Consiglio Nazionale delle Ricerche-Dipartimento di Scienze Biomediche, Istituto di Fisiologia Clinica, Area della Ricerca CNR, Via Moruzzi 1, 56124, Pisa, Italy

    Renata Del Carratore

  4. INRA, UMR1319 Micalis, 78350, Jouy-en-Josas, France

    Didier Chevret

  5. AgroParisTech, UMR Micalis, 78350, Jouy-en-Josas, France

    Didier Chevret

  6. Dipartimento di Agraria, University of Sassari, 07100, Sassari, Italy

    Quirico Migheli

  7. Unità di Ricerca, Istituto Nazionale di Biostrutture e Biosistemi, University of Sassari, Viale Italia 39, 07100, Sassari, Italy

    Quirico Migheli

Authors
  1. Biancaelena Maserti
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  2. Alessandra Podda
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  3. Lucia Giorgetti
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  4. Renata Del Carratore
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  5. Didier Chevret
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  6. Quirico Migheli
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Corresponding author

Correspondence to Biancaelena Maserti.

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Handling Editor: P. R. Jungblut.

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Maserti, B., Podda, A., Giorgetti, L. et al. Proteome changes during yeast-like and pseudohyphal growth in the biofilm-forming yeast Pichia fermentans . Amino Acids 47, 1091–1106 (2015). https://doi.org/10.1007/s00726-015-1933-1

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  • DOI: https://doi.org/10.1007/s00726-015-1933-1

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