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Taurine resumed neuronal differentiation in arsenite-treated N2a cells through reducing oxidative stress, endoplasmic reticulum stress, and mitochondrial dysfunction

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Abstract

The goal of the study is to investigate the preventive effect of taurine against arsenite-induced arrest of neuronal differentiation in N2a cells. Our results revealed that taurine reinstated the neurite outgrowth in arsenite-treated N2a cells. Meanwhile, arsenite-induced oxidative stress and mitochondrial dysfunction as well as degradation of mitochondria DNA (mtDNA) were also inhibited by co-treatment of taurine. Since oxidative stress and mitochondrial dysfunction is closely associated with endoplasmic reticulum (ER) stress, we further examined indicators of ER stress, 78 kDa glucose-regulated protein (GRP78), and C/EBP-homologous protein (CHOP) protein expression. The results demonstrated that taurine significantly reduced arsenite-induced ER stress in N2a cells. In the parallel experiment, arsenite-induced disruption of intracellular calcium homeostasis was also ameliorated by taurine. The proven bio-function of taurine preserved a preventive effect against deleteriously cross-talking between oxidative stress, mitochondria, and ER. Overall, the results of the study suggested that taurine reinstated neuronal differentiation by inhibiting oxidative stress, ER stress, and mitochondrial dysfunction in arsenite-treated N2a cells.

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Acknowledgments

This study was supported by the National Science Council, Taiwan, and the Center of Excellence for Marine Bioenvironment and Biotechnology.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Food Science, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung, 202, Taiwan, ROC

    Chien-Te Chou, Hong-Ting Lin & Deng-Fwu Hwang

  2. Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, ROC

    Shang-Ta Wang

  3. Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan, ROC

    Cheng-Hong Hsieh & Deng-Fwu Hwang

  4. Seafood Technology Division, Fisheries Research Institute, Council of Agriculture, Keelung, Taiwan, ROC

    Pai-An Hwang

Authors
  1. Chien-Te Chou
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  2. Hong-Ting Lin
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  4. Shang-Ta Wang
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  5. Cheng-Hong Hsieh
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Correspondence to Deng-Fwu Hwang.

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Chou, CT., Lin, HT., Hwang, PA. et al. Taurine resumed neuronal differentiation in arsenite-treated N2a cells through reducing oxidative stress, endoplasmic reticulum stress, and mitochondrial dysfunction. Amino Acids 47, 735–744 (2015). https://doi.org/10.1007/s00726-014-1901-1

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  • DOI: https://doi.org/10.1007/s00726-014-1901-1

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