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Recent advances in the molecular biology of metazoan polyamine transport

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Abstract

Very limited molecular knowledge exists about the identity and protein components of the ubiquitous polyamine transporters found in animal cells. However, a number of reports have been published over the last 5 years on potential candidates for metazoan polyamine permeases. We review the available evidence on these putative polyamine permeases, as well as establish a useful «identikit picture» of the general polyamine transport system, based on its properties as found in a wide spectrum of mammalian cells. Any molecular candidate encoding a putative «general» polyamine permease should fit that provided portrait. The current models proposed for the mechanism of polyamine internalization in mammalian cells are also briefly reviewed.

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Notes

  1. The abbreviations used are: PMPP, plasma membrane polyamine permeases; PTS, polyamine transport system; ODC, ornithine decarboxylase; DFMO, 2-difluoromethylornithine; CHO cells, Chinese hamster ovary cells; OAZ, ornithine decarboxylase antizyme; AZIN, antizyme inhibitor; SSAT, spermidine/spermine N 1-acetyltransferase; PSVs, polyamine-sequestering vesicles; RME, receptor-mediated endocytosis; HMA, 5-(N,N-hexamethylene)amiloride; Spd-MANT, N-spermidine-[N 4-(3-aminopropyl)] anthranylamide.

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Acknowledgments

Part of the work mentioned in this review that was performed by the authors was made possible through various grants from the Natural Sciences and Engineering Research Council of Canada, the Canadian Institutes of Health Research, the Cancer Research Society-Quebec and the National Institutes of Health (USA).

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Authors and Affiliations

  1. Department of Molecular Biology, Medical Biochemistry and Pathology, Faculty of Medicine, Laval University, Quebec, Canada

    R. Poulin

  2. Department of Psychiatry, Faculty of Medicine, Laval University, Quebec, Canada

    D. Soulet

  3. The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, The Johns Hopkins University School of Medicine, Baltimore, MD, 21231, USA

    R. A. Casero

  4. Endocrinology and Genomics Division, CHUQ Medical Research Center (CHUL Pavilion), T-0-37, 2705 Laurier Blvd, Quebec, G1V-4G2, Canada

    R. Poulin

  5. Neurological Science Division, CHUQ Medical Research Center (CHUL Pavilion), Quebec, Canada

    D. Soulet

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  1. R. Poulin
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  3. D. Soulet
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Correspondence to R. Poulin.

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Poulin, R., Casero, R.A. & Soulet, D. Recent advances in the molecular biology of metazoan polyamine transport. Amino Acids 42, 711–723 (2012). https://doi.org/10.1007/s00726-011-0987-y

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