Skip to main content
SpringerLink
Log in

Polyamines are absorbed through a y+ amino acid carrier in rat intestinal epithelial cells

  • Published:
Amino Acids Aims and scope Submit manuscript

Summary.

Due to the similarity in transport characteristics of polyamines and the y+ basic amino acid system, we hypothesized that both substrates could be moving through a common carrier site. Competitive and cross inhibition experiments in intestinal epithelial cells revealed the possibility of a common transport site. N-ethylmalemide (NEM) inhibited both lysine and putrescine transport, confirming that both were carried by a y+ transporter. Overexpressing the y+ transporter CAT-1 in a polyamine transport-deficient cell line, CHO-MG, did not reconstitute polyamine-transport. Thus, polyamines are not traveling through CAT-1. To determine if lysine is carried by a polyamine transport site, an antizyme-overexpressing cell line was used. Antizyme overexpression decreased polyamine uptake by 50%; in contrast, lysine transport was unaffected. Therefore, lysine is not traveling through a polyamine transport site. It appears that polyamines and lysine are likely traveling through a common unknown y+ transport site.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • FM Aususel R Brent RE Kingston DD Moore JG Seidman JA Smith K Struhl (1996) Current protoclos in molecular biology, Suppl 36. John Wiley and Sons Inc New York 9.1.1–9.1.11

    Google Scholar 

  • T Byers R Wechter M Nuttall A Pegg (1989) ArticleTitleExpression of human gene for polyamine transport in Chinese-hamster ovary cells. Biochem J 263 745–752 Occurrence Handle2512913 Occurrence Handle1:CAS:528:DyaK3cXhvFGr

    PubMed  CAS  Google Scholar 

  • HN Christensen (1964) ArticleTitleA transport system serving for mono- and diamino acids. Proc Natl Acad Sci USA 51 337–344 Occurrence Handle14124332 Occurrence Handle1:CAS:528:DyaF2cXosFajsg%3D%3D

    PubMed  CAS  Google Scholar 

  • EI Closs (1996) ArticleTitleCATs, a family of three distinct mammalian cationic amino acid transporters. Amino Acids 11 193–208 Occurrence Handle1:CAS:528:DyaK28XntlWksrc%3D

    CAS  Google Scholar 

  • EI Closs (2002) ArticleTitleExpression, regulation, and function of carrier proteins for cationic amino acids. Curr Opin Nephrol Hypertens 11 99–107 Occurrence Handle10.1097/00041552-200201000-00015 Occurrence Handle11753094

    Article  PubMed  Google Scholar 

  • R Deves CA Boyd (1998) ArticleTitleTransporters for cationic amino acids in animal cells: discovery, structure, and function. Physiol Rev 78 487–545 Occurrence Handle9562037 Occurrence Handle1:CAS:528:DyaK1cXislakurs%3D

    PubMed  CAS  Google Scholar 

  • R Deves S Angelo P Chavez (1993) ArticleTitleN-ethylmaleimide discriminates between two lysine transport systems in human erythrocytes. J Physiol 468 753–766 Occurrence Handle8254535 Occurrence Handle1:CAS:528:DyaK3sXmsVKkt7g%3D

    PubMed  CAS  Google Scholar 

  • G Groblewski P Hargittai ER Seidel (1992) ArticleTitleCa++/Calmodulin regulation of putrescine uptake in cultured gastrointestinal epithelial cells. Am J Physiol Cell Physiol 262 C1356–C1363 Occurrence Handle1:CAS:528:DyaK38XltVyks7c%3D

    CAS  Google Scholar 

  • K Igarashi K Kashiwagi (1996) ArticleTitlePolyamine transport in bacteria and yeast. Biochem J 344 633–642

    Google Scholar 

  • DL Jack IT Paulsen MH Saier (2000) ArticleTitleThe amino acid/polyamine/organocation (APC) superfamily of transporters specific for amino acids, polyamines and organocations. Microbiology 146 1797–1814 Occurrence Handle10931886 Occurrence Handle1:CAS:528:DC%2BD3cXmtVemsLw%3D

    PubMed  CAS  Google Scholar 

  • T Kameji S-I Hayashi K Hoshino Y Kakinuma K Igarashi (1993) ArticleTitleMultiple regulation of ornithine decarboxylase in enzyme-overproducing cells. Biochem J 289 581–586 Occurrence Handle8424799 Occurrence Handle1:CAS:528:DyaK3sXnslOgsA%3D%3D

    PubMed  CAS  Google Scholar 

  • K Kashiwagi A Kuraishi H Tomitori A Igarashi K Nishimura A Shirahata K Igarashi (2000) ArticleTitleIdentification of the putrescine recognition siteon polyamine transport protein PotE. J Biol Chem 275 36007–36012 Occurrence Handle10.1074/jbc.M006083200 Occurrence Handle10964926 Occurrence Handle1:CAS:528:DC%2BD3cXosVSktbo%3D

    Article  PubMed  CAS  Google Scholar 

  • JW Kim EI Closs LM Albritton JM Cunningham (1991) ArticleTitleTransport of cationic amino acids by the mouse ecotropic retrovirus receptor. Nature 352 725–728 Occurrence Handle10.1038/352725a0 Occurrence Handle1652100 Occurrence Handle1:CAS:528:DyaK3MXlvVSjsb4%3D

    Article  PubMed  CAS  Google Scholar 

  • CL MacLeod (1996) ArticleTitleRegulation of cationic amino acid transporter (CAT) gene expression. Biochem Soc Trans 24 846–852 Occurrence Handle8878860 Occurrence Handle1:CAS:528:DyaK28XlslSnsr8%3D

    PubMed  CAS  Google Scholar 

  • MS Malandro MS Kilberg (1996) ArticleTitleMolecular biology of mammalian amino acid transporters. Annu Rev Biochem 65 305–336 Occurrence Handle10.1146/annurev.bi.65.070196.001513 Occurrence Handle8811182 Occurrence Handle1:CAS:528:DyaK28XktFamtLk%3D

    Article  PubMed  CAS  Google Scholar 

  • JL Mandel WF Flintoff (1978) ArticleTitleIsolation of mutant mammalian cells altered in polyamine transport. J Cell Physiol 97 335–343 Occurrence Handle10.1002/jcp.1040970308 Occurrence Handle730773 Occurrence Handle1:CAS:528:DyaE1MXhvF2juw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • M McNemar J Gorman H Buckley (2001) ArticleTitleIsolation of a gene encoding a putative polyamine transporter from Candida albicans, GPT1. Yeast 18 555–561 Occurrence Handle10.1002/yea.697 Occurrence Handle11284011 Occurrence Handle1:CAS:528:DC%2BD3MXjtVehtLs%3D

    Article  PubMed  CAS  Google Scholar 

  • MA Medina JL Urdiales JM Mates I Nunez de Castro F Sanchez-Jimenez (1991) ArticleTitleDiamines interfere with the transport of L-ornithine in Ehrlich-cell plasma-membrane vesicles. Biochem J 280 825–827 Occurrence Handle1764045 Occurrence Handle1:CAS:528:DyaK38XhslarsQ%3D%3D

    PubMed  CAS  Google Scholar 

  • JL Mitchell CY Choe GG Judd DJ Daghfal RJ Kurzeja A Leyser (1996) ArticleTitleOverproduction of stable ornithine decarboxylase and antizyme in the difluoromethylornithine-resistant cell line DH23b. Biochem J 317 811–816 Occurrence Handle8760367 Occurrence Handle1:CAS:528:DyaK28XltlSgurw%3D

    PubMed  CAS  Google Scholar 

  • D Osborne ER Seidel (1990) ArticleTitleGastrointestinal luminal polyamines: cellular accumulation and enterohepatic circulation. Am J Physiol Gastrointest Liver Physiol 258 G576–G584 Occurrence Handle1:CAS:528:DyaK3cXitlart78%3D

    CAS  Google Scholar 

  • M Palacin R Estevez J Bertran A Zorzano (1998) ArticleTitleMolecular biology of mammaliam plasma membrane amino acid transporters. Physiol Rev 78 969–1054 Occurrence Handle9790568 Occurrence Handle1:CAS:528:DyaK1cXntFOhurg%3D

    PubMed  CAS  Google Scholar 

  • M Pan M Malandro B Stevens (1995) ArticleTitleRegulation of system y+ arginine transport capacity in differentiating human intestinal Caco-2 cells. Am J Physiol Gastrointest Liver Physiol 268 G578–G585 Occurrence Handle1:CAS:528:DyaK2MXlt1yitLo%3D

    CAS  Google Scholar 

  • A Pegg (1988) ArticleTitlePolyamine metabolism and its importance in neoplastic growth and as a target for chemotherapy. Cancer Res 48 759–774 Occurrence Handle3123052 Occurrence Handle1:CAS:528:DyaL1cXht1eqs7w%3D

    PubMed  CAS  Google Scholar 

  • LD Possaine BM Martin PL Fletcher SuffixJr (1991) ArticleTitleDischarge effect on pancreatic exocrine secretion produced by toxins purified from Tityus serrulatus scorpion venom. J Biol Chem 266 3178–3185

    Google Scholar 

  • AM Rojas R Deves (1999) ArticleTitleMammalian amino acid transport system y+ revisited: specifity and cation dependence of the interaction with neutral amino acids. J Membr Biol 186 199–208

    Google Scholar 

  • MH Saier (2000) ArticleTitleFamilies of transmembrane transporters selective for amino acids and their derivatives. Microbiology 146 1782–1784

    Google Scholar 

  • K Sakata T Fukuchi-Shimogori K Kashiwagi K Igarashi (1997) ArticleTitleIdentification of regulatory region of antizyme necessary for the negative regulation of polyamine transport. Biochem Biophys Res Commun 238 415–419 Occurrence Handle10.1006/bbrc.1997.7266 Occurrence Handle9299523 Occurrence Handle1:CAS:528:DyaK2sXmsVGksbg%3D

    Article  PubMed  CAS  Google Scholar 

  • K Sakata K Kashiwagi K Igarashi (2000) ArticleTitleProperties of a polyamine transporter regulated by antizyme. Biochem J 347 297–303 Occurrence Handle10.1042/0264-6021:3470297 Occurrence Handle10727431 Occurrence Handle1:CAS:528:DC%2BD3cXislSms7g%3D

    Article  PubMed  CAS  Google Scholar 

  • JL Scemama V Grabie ER Seidel (1993) ArticleTitleCharacterization of univectorial polyamine transport in duodenal crypt cell line. Am J Physiol Gastrointest Liver Physiol 265 G851–G856 Occurrence Handle1:CAS:528:DyaK2cXltVyksQ%3D%3D

    CAS  Google Scholar 

  • ER Seidel JL Scemama (1997) ArticleTitleGastrointestinal polyamines and regulation of mucosal growth and function. Nutritional Biochem 8 104–111 Occurrence Handle1:CAS:528:DyaK2sXjtVyksbs%3D

    CAS  Google Scholar 

  • N Seiler F Dezeure (1990) ArticleTitlePolyamine transport in mammalian cells. Int J Biochem 22 211–218 Occurrence Handle10.1016/0020-711X(90)90332-W Occurrence Handle2110083 Occurrence Handle1:CAS:528:DyaK3cXhslGqtb4%3D

    Article  PubMed  CAS  Google Scholar 

  • N Seiler JG Delcros JP Moulinoux (1996) ArticleTitlePolyamine transport in mammalian cells. An update. Int J Biochem Cell Biol 28 843–861 Occurrence Handle10.1016/1357-2725(96)00021-0 Occurrence Handle8811834 Occurrence Handle1:CAS:528:DyaK28XlvVGnsbY%3D

    Article  PubMed  CAS  Google Scholar 

  • N Seiler C Atanassov F Raul (1998) ArticleTitlePolyamine metabolism as target for cancer chemoprevention. Int J Oncol 13 993–1006 Occurrence Handle9772292 Occurrence Handle1:CAS:528:DyaK1cXnsV2hsrc%3D

    PubMed  CAS  Google Scholar 

  • T Suzuki Y He K Kashiwagi Y Murakami S Hayashi K Igarashi (1994) ArticleTitleAntizyme protects against abnormal accumulation and toxicity of polyamines in ornithine decarboxylase-overproducing cells. Proc Natl Acad Sci USA 91 8930–8934 Occurrence Handle8090747 Occurrence Handle1:CAS:528:DyaK2cXlvFGrurY%3D

    PubMed  CAS  Google Scholar 

  • M Torras-Llort R Ferrer JF Soriano-Garcia M Moreto (1996) ArticleTitleL-Lysine transport in chicken jejunal brush border membrane vesicles. J Membr Biol 152 183–193 Occurrence Handle10.1007/s002329900096 Occurrence Handle8672084 Occurrence Handle1:CAS:528:DyaK28XltVaktrs%3D

    Article  PubMed  CAS  Google Scholar 

  • MF White HN Christensen (1982) ArticleTitleCationic amino acid transport into cultured animal cells. J Biol Chem 257 4450–4457 Occurrence Handle7068644 Occurrence Handle1:CAS:528:DyaL38XitFGgtrs%3D

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology, Brody School of Medicine, East Carolina University, Greenville, North Carolina, U.S.A.

    J. G. Sharpe & E. R. Seidel

Authors
  1. J. G. Sharpe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. R. Seidel
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sharpe, J., Seidel, E. Polyamines are absorbed through a y+ amino acid carrier in rat intestinal epithelial cells. Amino Acids 29, 245–253 (2005). https://doi.org/10.1007/s00726-005-0234-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00726-005-0234-5

Search

Navigation