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Comparative proteomics analysis of differentially expressed proteins in soybean cell wall during flooding stress

Amino Acids volume 39, pages 1435–1449 (2010)Cite this article

Abstract

Flooding is a major problem for soybean crop as it reduces the growth and grain yield. To investigate the function of the soybean cell wall in the response to flooding stress, cell wall proteins were analyzed. Cell wall proteins from roots and hypocotyls of soybeans, which were germinated for 2 days and subjected to 2 days of flooding, were purified, separated by two-dimensional polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue. Sixteen out of 204 cell wall proteins showed responses to flooding stress. Of these, two lipoxygenases, four germin-like protein precursors, three stem 28/31 kDa glycoprotein precursors, and one superoxide dismutase [Cu–Zn] were downregulated. A copper amine oxidase was found to have shifted from the basic to acidic zone following flooding stress. Based on these results, it was confirmed by the lignin staining that the lignification was suppressed in the root of soybean under the flooding stress. These results suggest that the roots and hypocotyls of soybean caused the suppression of lignification through decrease of these proteins by downregulation of reactive oxygen species and jasmonate biosynthesis under flooding stress.

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Abbreviations

2-DE:

Two-dimensional polyacrylamide gel electrophoresis

MS:

Mass spectrometry

CBB:

Coomassie brilliant blue

pI:

Isoelectric point

PMSF:

Phenylmethyl sulfonyl fluoride

PVP:

Polyvinylpolypyrrolidone

G6PDH:

Glucose-6-phosphate dehydrogenase

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Acknowledgments

We thank Mr. M. Z. Nouri and Dr. F.-J. Kong for their experimental help. We also thank Dr. S. Shimamura and T. Nakamura for their valuable discussion. This work was supported by a Grant-in-Aid for Scientific Research (B) (19380015) of the Japan Society for the Promotion of Science.

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Affiliations

  1. National Institute of Crop Science, Kannondai 2-1-18, Tsukuba, 305-8518, Japan

    Setsuko Komatsu, Yukito Kobayashi, Keito Nishizawa & Yohei Nanjo

  2. Nagaoka University of Technology, Nagaoka, 940-2188, Japan

    Yukito Kobayashi & Kiyoshi Furukawa

Authors
  1. Setsuko Komatsu
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  2. Yukito Kobayashi
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  3. Keito Nishizawa
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  4. Yohei Nanjo
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  5. Kiyoshi Furukawa
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Corresponding author

Correspondence to Setsuko Komatsu.

Additional information

S. Komatsu and Y. Kobayashi contributed equally to the present study.

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Komatsu, S., Kobayashi, Y., Nishizawa, K. et al. Comparative proteomics analysis of differentially expressed proteins in soybean cell wall during flooding stress. Amino Acids 39, 1435–1449 (2010). https://doi.org/10.1007/s00726-010-0608-1

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  • DOI: https://doi.org/10.1007/s00726-010-0608-1

Keywords

  • Cell wall
  • Flooding
  • Lignification
  • Proteome
  • Soybean