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The inhibitory effect of various indolyl amino acid derivatives on arginase activity in macrophages

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Summary.

Numerous indolyl amino acids and their derivatives inhibited arginase activity. The inhibition was found to be non-competitive, – at least partly – allosteric, and independent on manganese ions in the active site, and it cannot be explained by the dissociation of arginase homotrimers. Indole alone is weakly inhibitory; however, the presence of three-carbon side chains and their net charges is favorable for the inhibition. The binding of the inhibitory compounds caused only minor changes in the steric structure of arginase: a slight increase in α-helix content was detected by circular dichroism together with a decrease in parallel pleated sheet and β-turn sections. A slight alteration in the tertiary structure was also found using tryptophane fluorescence studies, but buried apolar side chains were not transposed to the protein surface. Computer studies that were performed did not provide additional structural information.

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Abbreviations

ANOVA:

Analysis of Variances between groups

ANS:

8-anilino-1-naphthalenesulfonic acid

DMEM:

Dulbecco Minimal Essential Medium

FBS:

fetal bovine serum

NOS:

nitric oxide synthase

SDS:

sodium dodecyl sulfate

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Authors and Affiliations

  1. Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University Medical School, Budapest, Hungary

    A. Hrabák, T. Bajor & G. Mészáros

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  1. A. Hrabák
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  2. T. Bajor
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  3. G. Mészáros
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Authors’ address: András Hrabák, Department of Medical Chemistry, Molecular Biology and Patho-biochemistry, Semmelweis University Medical School, Budapest, VIII. Puskin u. 9., H-1444 POB 260, Hungary

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Hrabák, A., Bajor, T. & Mészáros, G. The inhibitory effect of various indolyl amino acid derivatives on arginase activity in macrophages. Amino Acids 34, 293–300 (2008). https://doi.org/10.1007/s00726-006-0470-3

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  • DOI: https://doi.org/10.1007/s00726-006-0470-3

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