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The apoptotic effect of intercalating agents on HPV-negative cervical cancer C-33A cells

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Summary.

Cervical cancer is one of the leading causes of female cancer death worldwide with about 500,000 deaths per year. Both mitomycin C and cisplatin are alkylating agents, which bind and intercalate DNA, and thus used as anti-cancer drugs. In these studies, we focused on investigating the apoptotic effects of intercalating agents on HPV-negative cervical cancer C-33A cells. Accordingly, C-33A cells were treated with carboplatin, mitomycin C or cisplatin. Cell cycle analysis revealed that treatment with mitomycin C and cisplatin but not with carboplatin resulted in apoptosis. Both mitomycin C and cisplatin induced apoptosis in C-33A cells via caspase-8 and -3 processing in a Fas/FasL-dependent manner and also suppressed IL-18 expression, while they down-regulated IκB expression and up-regulated p65 expression. These results suggest that both mitomycin C and cisplatin induce apoptosis, not only via the caspase-8 and -3 dependent Fas/FasL pathway, but also via the regulation of NF-κB activity and IL-18 expression in HPV-negative cervical cancer C-33A cells.

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Abbreviations

IFN-γ:

interferon-γ

FasL:

Fas ligand

NF-κB:

nuclear facor-κB

HPV:

human papillomavirus

MMC:

mitomycin C

CIS:

cisplatin

CA:

carboplatin

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Konkuk University, Seoul, South Korea

    Yun Hee Kang, Kyung-Ac Lee & Do-Young Yoon

  2. Department of Biology, Chungnam National University, Daejeon, South Korea

    Yun Hee Kang & Sang-Gi Paik

  3. Department of Biological Sciences, Sookmyung Women’s University, Seoul, South Korea

    Young Yang

  4. Institute of Biomedical Science and Technology, Konkuk University, Seoul, South Korea

    Soo-Hyun Kim & Do-Young Yoon

  5. Division of Genetic Toxicology, National Institute of Toxicological Research, Seoul, South Korea

    Joo Hwan Kim & Sue Nie Park

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  1. Yun Hee Kang
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  2. Kyung-Ac Lee
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Kang, Y., Lee, KA., Yang, Y. et al. The apoptotic effect of intercalating agents on HPV-negative cervical cancer C-33A cells. Amino Acids 33, 105–112 (2007). https://doi.org/10.1007/s00726-006-0417-8

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  • DOI: https://doi.org/10.1007/s00726-006-0417-8

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