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The effect of tryptophan administration on ileum contractility and oxidant status in mice

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Summary.

L-Tryptophan (TRP) is the precursor amino acid for the synthesis of serotonin (5-HT). 5-HT is effective both on the food intake and gastrointestinal system contractility. The aim of this study was to search the effects of systemic TRP treatment on 5-HT levels of ileum and searching the effect of ileal contractility and oxidant status. Swiss-albino mice were divided into two groups: 1. Control, 2. TRP-treated (100 mg/kg/24 h, i.p., for 7 days). Body weights were recorded at the beginning and at the end of experiments. Acetylcholine-induced contractile responses in the isolated ileum were recorded on polygraph. Ileal tissue malondialdehyde and glutathione levels determined by spectrophotometric and ileal tissue 5-HT levels were measured by immunohistochemical methods. TRP treatment decreased body weight and increased ileal contractile response. In the TRP-treated group, ileum malondialdehyde levels increased and glutathione levels decreased. Immunohistochemical detection showed that ileal 5-HT levels were increased by TRP treatment. There is a relationship between increased oxidative stress and increased contractility in the ileal tissue of the TRP-treated animals. These effects may be related to increased ileal 5-HT synthesis.

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References

  • M Aviram U Cogan S Modaky (1991) ArticleTitleExcessive dietary tryptophan enhances plasma lipid peroxidation in rats Atherosclerosis 88 29–34 Occurrence Handle1715163 Occurrence Handle10.1016/0021-9150(91)90254-Z Occurrence Handle1:CAS:528:DyaK3MXmtlWjsbw%3D

    Article  PubMed  CAS  Google Scholar 

  • AG Aykaç M Uysal AS Yalçin N Koçak-Toker A Sivas H Öz (1985) ArticleTitleThe effect of chronic ethanol ingestion on hepatic lipid peroxide, glutathione, glutathione peroxidase and glutathione transferase in rats Toxicology 36 71–76 Occurrence Handle4040665 Occurrence Handle10.1016/0300-483X(85)90008-3

    Article  PubMed  Google Scholar 

  • MR Bennett (2000) ArticleTitleThe concept of transmitter receptors: 100 years on Neuropharmacology 39 523–546 Occurrence Handle10728874 Occurrence Handle10.1016/S0028-3908(99)00137-9 Occurrence Handle1:CAS:528:DC%2BD3cXhtFWqsr4%3D

    Article  PubMed  CAS  Google Scholar 

  • A Casini M Ferrali A Pompella (1986) ArticleTitleLipid peroxidation and cellular damage in extrahepatic tissue of bromobenzene intoxicated mice Am J Pathol 123 520–531 Occurrence Handle3717304 Occurrence Handle1:CAS:528:DyaL28XksFGqs7o%3D

    PubMed  CAS  Google Scholar 

  • V Denes Z Lazar L Bartho R Gabriel (2003) ArticleTitleSerotonin in the rabbit ileum: localization, uptake and effect on motility Anat Rec 271A 368–376 Occurrence Handle10.1002/ar.a.10042 Occurrence Handle1:CAS:528:DC%2BD3sXjtVChtL4%3D

    Article  CAS  Google Scholar 

  • O Ekwall H Hedstrand L Grimelius J Haavik J Perheentupa J Gustafsson E Husebye O Kampe F Rorsman (1998) ArticleTitleIdentification of tryptophan hydroxylase as an intestinal autoantigen Lancet 352 279–283 Occurrence Handle9690408 Occurrence Handle10.1016/S0140-6736(97)11050-9 Occurrence Handle1:CAS:528:DyaK1cXltlegsbw%3D

    Article  PubMed  CAS  Google Scholar 

  • T Eriksson J Walinder (1998) ArticleTitleAmitriptyline and clomipramine increase the concentration of administered L-tryptophan in the rat brain J Pharm Pharmacol 50 1133–1137 Occurrence Handle9821660 Occurrence Handle1:CAS:528:DyaK1cXntFWisbo%3D

    PubMed  CAS  Google Scholar 

  • JD Fernstrom RJ Wurtman (1972) ArticleTitleBrain serotonin content: physiological regulation by plasma neutral amino acids Science 178 414–416 Occurrence Handle5077329 Occurrence Handle10.1126/science.178.4059.414 Occurrence Handle1:CAS:528:DyaE3sXjtV2h

    Article  PubMed  CAS  Google Scholar 

  • PC Grisotto AC dos Santos J Coutinho-Netto J Cherri CE Piccinato (2000) ArticleTitleIndicators of oxidative injury and alterations of the cell membrane in the skelatal muscle of rats submitted to ischemia and reperfusion J Surg Res 92 1–6 Occurrence Handle10864473 Occurrence Handle10.1006/jsre.2000.5823 Occurrence Handle1:CAS:528:DC%2BD3cXktFehtbw%3D

    Article  PubMed  CAS  Google Scholar 

  • G Huether B Poeggeler A Reimer A George (1992) ArticleTitleEffect of tryptophan administration on circulating melatonin levels in chicks and rats: evidence for stimulation of melatonin synthesis and release in the gastrointestinal tract Life Sci 51 945–953 Occurrence Handle1518369 Occurrence Handle10.1016/0024-3205(92)90402-B Occurrence Handle1:CAS:528:DyaK38XlsVeqtbg%3D

    Article  PubMed  CAS  Google Scholar 

  • RK Kurup PA Kurup (2002) ArticleTitleHypothalamic digoxin, cerebral dominance and mitochondrial function/free radical metabolism Int J Neurosci 112 1409–1421 Occurrence Handle12652894 Occurrence Handle10.1080/00207450290158296

    Article  PubMed  Google Scholar 

  • SH Lee WW Wang BL Fanburg (1998) ArticleTitleSuperoxide as an intemediate signal for serotonin-induced mitogenesis Free Rad Biol Med 24 855–858 Occurrence Handle9586816 Occurrence Handle10.1016/S0891-5849(97)00359-6

    Article  PubMed  Google Scholar 

  • SF Leibowitz G Shor-Posner (1986) ArticleTitleBrain serotonin and eating behavior Appetite Suppl 7 1–14

    Google Scholar 

  • M Nakajima Y Shiihara Y Shiba I Sano T Sakai A Mizumoto Z Itoh (1997) ArticleTitleEffect of 5-hydroxytryptamine on gastrointestinal motility in conscious guinea-pigs Neurogastroenterol Motil 9 205–214 Occurrence Handle9430788 Occurrence Handle10.1046/j.1365-2982.1997.d01-56.x Occurrence Handle1:STN:280:DyaK1c%2Fpt1GqtA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • T Noguchi M Nishino R Kido (1973) ArticleTitleTryptophan 5-hydroxylase in rat intestine Biochem J 131 375–380 Occurrence Handle4541815 Occurrence Handle1:CAS:528:DyaE3sXpsVWqtA%3D%3D

    PubMed  CAS  Google Scholar 

  • Ç Özer B Gönül G Take (2002) The effect of tryptophan on the memory and brain serotonin levels. 1 SeriesTitleNeurologic Sciences Congress Abstract Book Eskişehir, Turkey 76

    Google Scholar 

  • Ç Özer B Gönül G Take D Erdoğan E Tong ZS Ercan (2004) ArticleTitleTryptophan administration increase contractility and change the ultrastructure of mice duodenum Amino Acids 27 215–220 Occurrence Handle15503228 Occurrence Handle10.1007/s00726-004-0098-0 Occurrence Handle1:CAS:528:DC%2BD2cXoslGis7c%3D

    Article  PubMed  CAS  Google Scholar 

  • JC Peters (1991) Tryptophan nutrition and metabolism: an overview R Schwarcz (Eds) Kynurenine and serotonin pathways Plenum Press New York 345–358

    Google Scholar 

  • BA Rotter DB Prelusky BK Thompson (1996) ArticleTitleThe role of tryptophan in DON-induced feed rejection J Environm Sci Health 31 1279–1288 Occurrence Handle1:STN:280:ByiD2M7mvF0%3D Occurrence Handle10.1080/03601239609373067

    Article  CAS  Google Scholar 

  • G Sabeur (1996) ArticleTitleEffect of temparature on the contractile response of isolated rat small intestine to acetlycholine and KCl: calcium dependence Arch Physiol Biochem 104 220–228 Occurrence Handle8818208 Occurrence Handle10.1076/apab.104.2.220.12891 Occurrence Handle1:CAS:528:DyaK28Xkt1Cksrg%3D

    Article  PubMed  CAS  Google Scholar 

  • MT Salvador MD Murillo MC Rodriguez-Yoldi AI Alcalde JE Mesonero M Rodriguez-Yoldi (2000) ArticleTitleEffect of serotonin on the physiology of the rabbit small intestine Can J Physiol Pharmacol 78 359–366 Occurrence Handle10841430 Occurrence Handle10.1139/cjpp-78-5-359 Occurrence Handle1:CAS:528:DC%2BD3cXjsVKnsb4%3D

    Article  PubMed  CAS  Google Scholar 

  • G Sener O Tosun AO Sehirli A Kaçmaz S Arbak Y Ersoy G Ayanoğlu-Dülger (2003) ArticleTitleMelatonin and N-acetylcysteine have beneficial effects during hepatic ischemia and reperfusion Life Sci 72 2707–2718 Occurrence Handle12679188 Occurrence Handle10.1016/S0024-3205(03)00187-5 Occurrence Handle1:CAS:528:DC%2BD3sXisV2ksL4%3D

    Article  PubMed  CAS  Google Scholar 

  • K Taniyama N Makimato A Furuichi Y Sakurai-Yamashita Y Nagase M Kaibara T Kanematsu (2000) ArticleTitleFunctions of peripheral 5-hydroxy-tryptamine 4 receptor in gastrointestinal motility J Gastroenterol 35 575–582 Occurrence Handle10955595 Occurrence Handle10.1007/s005350070056 Occurrence Handle1:CAS:528:DC%2BD3cXmtFOmsLk%3D

    Article  PubMed  CAS  Google Scholar 

  • I Yamamato A Kuwahara M Fujimura M Kadowaki M Fujimiya (1999) ArticleTitleInvolvement of 5-HT3 and 5-HT4 receptors in the motor activity of isolated vascularly perfused rat duodenum Neurogastroenterol Motil 11 457–465 Occurrence Handle10.1046/j.1365-2982.1999.00173.x

    Article  Google Scholar 

  • T Yasuhiro RP Korolkiewicz S Kato K Takeuchi (1997) ArticleTitleRole of nitric oxide in pathogenesis of serotonine-induced gastric lessions in rats Pharmacol Res 36 333–338 Occurrence Handle9425624 Occurrence Handle10.1006/phrs.1997.0238 Occurrence Handle1:CAS:528:DyaK1cXjsl2htw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • PL Yu M Fujimura K Okumiya M Kinoshita H Hasegawa M Fujimiya (1999) ArticleTitleImmunohistochemical localization of tryptophan hydroxylase in the human and rat gastrointestinal tracts J Comp Neurol 411 654–665 Occurrence Handle10421874 Occurrence Handle10.1002/(SICI)1096-9861(19990906)411:4<654::AID-CNE9>3.0.CO;2-H Occurrence Handle1:CAS:528:DyaK1MXlsVCrt7g%3D

    Article  PubMed  CAS  Google Scholar 

  • X Zhao JS Alexander S Zhang Y Zhu NJ Sieber JT Aw LD Carden (2001) ArticleTitleRedox regulation of endothelial barrier integrity Am J Physiol Lung Cell Mol Physiol 281 879–886

    Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, University of Gazi, Ankara, Turkey

    Ç. Özer & B. Gönül

  2. Department of Pharmacology, Faculty of Medicine, University of Gazi, Ankara, Turkey

    Z. S. Ercan

  3. Department of Histology and Embryology, Faculty of Medicine, University of Gazi, Ankara, Turkey

    G. Take & D. Erdoğan

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  1. Ç. Özer
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  2. B. Gönül
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  3. Z. S. Ercan
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  4. G. Take
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  5. D. Erdoğan
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Özer, Ç., Gönül, B., Ercan, Z. et al. The effect of tryptophan administration on ileum contractility and oxidant status in mice. Amino Acids 32, 453–458 (2007). https://doi.org/10.1007/s00726-006-0339-5

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  • DOI: https://doi.org/10.1007/s00726-006-0339-5

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